Endothelial cell osteogenesis induced differentiation culture medium and preparation method thereof

A technique for inducing differentiation of endothelial cells, applied in the field of culture medium, can solve the problem of lack of endothelial cell differentiation medium, and achieve the effect of high degree of induction and differentiation, fast speed and simple operation

Inactive Publication Date: 2021-06-25
WUXI NO 9 PEOPLES HOSPITAL
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0002] At present, the existing osteogenic induction medium is mainly aimed at a variety of tissue-derived mesenchymal stem cells including bone marrow mesenchymal stem cells, umbilical cord mesenchymal stem cells, and adipose-derived mesenchymal stem cells. Media for Osteoblast Differentiation

Method used

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  • Endothelial cell osteogenesis induced differentiation culture medium and preparation method thereof
  • Endothelial cell osteogenesis induced differentiation culture medium and preparation method thereof
  • Endothelial cell osteogenesis induced differentiation culture medium and preparation method thereof

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Embodiment Construction

[0028] In order to make the technical means, creative features, goals and effects achieved by the present invention easy to understand, the present invention will be further described below in conjunction with specific embodiments.

[0029] All components involved in the present invention are conventional products on the market. The information in the specific implementation case is as follows:

[0030] The endothelial cell osteogenic differentiation medium of the present invention is made of the following components: α-MEM medium, fetal bovine serum 5-50% by volume, Insulin-Transferrin-Selenium (ITS-G) 0.1%-5% Volume percentage, bFGF 1-10ng / ml, EGF 1-10ng / ml, VEGF 5-50ng / ml, TGF-β 5-50ng / ml concentration, MSCGS 0.1%-5% volume percentage, sodium pyruvate 0.1-10mM, Mercaptoethanol 0.01-1mM, Dexamethasone 10-200nM, Vc10-150ug / ml, β-sodium glycerophosphate 1-100mM, L-glutamine 10-50mM, Penicillin 0.1%-5% by volume .

[0031] Preferably, the vascular endothelial cell osteogenic...

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Abstract

The invention discloses an endothelial cell osteogenesis induced differentiation culture medium and a preparation method, and belongs to the technical field of culture mediums. The culture medium is prepared from the following components in percentage by volume: an alpha-MEM culture medium, 5 to 50 percent of fetal calf serum, 0.1 to 5 percent of Insuin-Transferrin-Selenium (ITS-G), 1 to 10ng/ml of bFGF, 1 to 10ng/ml of EGF, 5 to 50ng/ml of VEGF, 5 to 50ng/ml of TGF-beta, 0.1 to 5 percent of MSCGS, 0.1 to 10mM of sodium pyruvate, 0.01 to 1mM of mercaptoethanol, 10 to 200nM of Dexametasone, 10 to 150ug/ml of Vc and 1 to 100mM of beta-sodium glycerophosphate. The culture medium is simple in preparation method, high in induction speed and stable in process.

Description

technical field [0001] The invention relates to the technical field of medium, in particular to an endothelial cell osteogenic differentiation medium and a preparation method thereof. Background technique [0002] At present, the existing osteogenic induction medium is mainly aimed at a variety of tissue-derived mesenchymal stem cells including bone marrow mesenchymal stem cells, umbilical cord mesenchymal stem cells, and adipose-derived mesenchymal stem cells. Medium for osteoblast differentiation. At present, the existing technology is to first induce the differentiation of endothelial cells into stem cells through different reagents, so that endothelial cells have the stemness of differentiation into osteoblasts, and then differentiate into osteoblasts through osteogenesis induction medium. This technology has a cost High, long cycle, and low success rate, now through the present invention, the characteristics of differentiation of endothelial cells to osteoblasts can be...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N5/077C12N5/071
CPCC12N5/0654C12N2506/28C12N2501/115C12N2501/11C12N2501/165C12N2501/15C12N2500/30C12N2500/44C12N2500/38C12N2500/42C12N2500/32
Inventor 毛栋芮永军糜菁熠潘筱云
Owner WUXI NO 9 PEOPLES HOSPITAL
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