Endothelial cell osteogenesis induced differentiation culture medium and preparation method thereof
A technique for inducing differentiation of endothelial cells, applied in the field of culture medium, can solve the problem of lack of endothelial cell differentiation medium, and achieve the effect of high degree of induction and differentiation, fast speed and simple operation
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[0028] In order to make the technical means, creative features, goals and effects achieved by the present invention easy to understand, the present invention will be further described below in conjunction with specific embodiments.
[0029] All components involved in the present invention are conventional products on the market. The information in the specific implementation case is as follows:
[0030] The endothelial cell osteogenic differentiation medium of the present invention is made of the following components: α-MEM medium, fetal bovine serum 5-50% by volume, Insulin-Transferrin-Selenium (ITS-G) 0.1%-5% Volume percentage, bFGF 1-10ng / ml, EGF 1-10ng / ml, VEGF 5-50ng / ml, TGF-β 5-50ng / ml concentration, MSCGS 0.1%-5% volume percentage, sodium pyruvate 0.1-10mM, Mercaptoethanol 0.01-1mM, Dexamethasone 10-200nM, Vc10-150ug / ml, β-sodium glycerophosphate 1-100mM, L-glutamine 10-50mM, Penicillin 0.1%-5% by volume .
[0031] Preferably, the vascular endothelial cell osteogenic...
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