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SNP molecular markers related to milk yield traits of dairy goats, liquid phase chip prepared from SNP molecular markers and application of liquid phase chip

A technology of molecular markers and dairy goats, applied in the field of molecular biology SNP technology and chips, can solve the problems of low seed selection efficiency, backwardness, and low renewal and elimination rates in dairy goat farms

Active Publication Date: 2021-07-23
INNER MONGOLIA AUTONOMOUS REGION ACAD OF AGRI & ANIMAL HUSBANDRY SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, the selection technology of dairy goats in my country is backward, and the sheep can only be bred to more than 12 months of age. After the lambs are bred and have milk, the quality of the lambs can be judged by the milk production, which makes the selection efficiency of dairy goat farms low, and the renewal and elimination rate is low. , and greatly increased the cost of feeding large-scale farms

Method used

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  • SNP molecular markers related to milk yield traits of dairy goats, liquid phase chip prepared from SNP molecular markers and application of liquid phase chip
  • SNP molecular markers related to milk yield traits of dairy goats, liquid phase chip prepared from SNP molecular markers and application of liquid phase chip
  • SNP molecular markers related to milk yield traits of dairy goats, liquid phase chip prepared from SNP molecular markers and application of liquid phase chip

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0049] SNP bit point determined

[0050] Deconstruction data for milk goats (sub-average yield 0.7kg) milk goat with high yield milk (secondary production of 2.7 kg) of milk goats (data acquisition information). HTTPS: / / www.ncbi. NLM.NIH.GOV / Genome / ?term=goat), combined with the characteristics and law of the 50K length of the gene, performing the allotential association analysis, sets the threshold of P <0.001, resulting in 83 SNP markers related to milk traits (Sequence information of the specific DGHMS01 to DGHMS83 is shown in Table 1), the determination of these sites is also the basis for the production of the following probe sequences and liquid phase chips.

Embodiment 2

[0052] Probe synthesis

[0053] The 83 SNP molecular marking sites obtained in Example 1 bonded to a 22 bp sequence design molecular probe adjacent to the athletic, each SNP molecule marking design 2 molecular probes. The specific molecular probe is shown in Table 1.

Embodiment 3

[0055] Preparation method of liquid phase probe

[0056] The molecular probe prepared in Example 2 plus a C12 molecular arm and an amino modification of the 5 'end of the sequence, and the modified molecular probe is coupled to different digitally encoded fluorescent microspheres (different data and molecular probe number) One correspondence to the number of each microsphere probe to more than 10,000, resulting in a specific fluorescent microsphere, a fluorescent microspheres coupled to each molecule probe are mixed to obtain a SNP molecule label detection liquid phase chip.

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Abstract

The invention provides SNP molecular markers related to milk yield traits of dairy goats, a liquid phase chip prepared from the SNP molecular markers and application of the liquid phase chip, and belongs to the technical field of molecular biological SNP technologies and chips. The invention provides the SNP molecular markers related to milk yield traits of dairy goats. The SNP molecular markers comprise the following cases: 1) one or more of the following 83 SNP molecular markers: SNP molecular markers DGHMS01 to DGHMS83; and 2) complementary sequences or homologous sequences of the SNP molecular markers in the case 1). A molecular probe set is designed according to the SNP molecular markers, and the liquid phase chip is prepared by using the molecular probe set. The milk yield of the dairy goats is detected by adopting the liquid phase chip, and result clustering analysis, principal component analysis and character statistical results show that the 83 molecular markers are closely related to the milk yield traits of the dairy goats. Therefore, the molecular probes are used for detecting the milk yield of the dairy goats and can be used for screening or identifying high-yield milk goat varieties.

Description

Technical field [0001] The present invention belongs to the field of molecular biological SNP techniques and chip technology, which relates to a SNP molecule marker and a liquid phase chip and application prepared therebetween with milky lamb produced. Background technique [0002] The milk goat industry is an important part of the development of my country's dairy industry. With the rapid temperature rise of the goat milk consumption market, the development of my country's milk goat industry is booming. Goat milk has become the second largest milk in my country. At present, the stock of Chinese milk goat is about 1.3 million, and the lact milk lamb is approximately 7.9 million, the total output of Goat milk is about 1.75 million tons, accounting for about 4.5% of fresh milk production. At the same time, the milk goat industry is moving rapidly from the traditional milk mountain farming model to modernize, intensive, standardized, and diverse quality efficiency mode. Goat milk is...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/6888C12N15/11
CPCC12Q1/6888C12Q2600/156C12Q2600/124Y02P60/87
Inventor 赵启南付少印孟子琪李长青
Owner INNER MONGOLIA AUTONOMOUS REGION ACAD OF AGRI & ANIMAL HUSBANDRY SCI
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