SNP molecular markers related to milk yield traits of dairy goats, liquid phase chip prepared from SNP molecular markers and application of liquid phase chip
A technology of molecular markers and dairy goats, applied in the field of molecular biology SNP technology and chips, can solve the problems of low seed selection efficiency, backwardness, and low renewal and elimination rates in dairy goat farms
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Embodiment 1
[0049] SNP bit point determined
[0050] Deconstruction data for milk goats (sub-average yield 0.7kg) milk goat with high yield milk (secondary production of 2.7 kg) of milk goats (data acquisition information). HTTPS: / / www.ncbi. NLM.NIH.GOV / Genome / ?term=goat), combined with the characteristics and law of the 50K length of the gene, performing the allotential association analysis, sets the threshold of P <0.001, resulting in 83 SNP markers related to milk traits (Sequence information of the specific DGHMS01 to DGHMS83 is shown in Table 1), the determination of these sites is also the basis for the production of the following probe sequences and liquid phase chips.
Embodiment 2
[0052] Probe synthesis
[0053] The 83 SNP molecular marking sites obtained in Example 1 bonded to a 22 bp sequence design molecular probe adjacent to the athletic, each SNP molecule marking design 2 molecular probes. The specific molecular probe is shown in Table 1.
Embodiment 3
[0055] Preparation method of liquid phase probe
[0056] The molecular probe prepared in Example 2 plus a C12 molecular arm and an amino modification of the 5 'end of the sequence, and the modified molecular probe is coupled to different digitally encoded fluorescent microspheres (different data and molecular probe number) One correspondence to the number of each microsphere probe to more than 10,000, resulting in a specific fluorescent microsphere, a fluorescent microspheres coupled to each molecule probe are mixed to obtain a SNP molecule label detection liquid phase chip.
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