Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Chinese sweetgum tissue culture and quick propagation method

A technology of tissue culture and sweetgum, applied in horticultural methods, botanical equipment and methods, horticulture, etc., can solve problems that have not yet been published with a complete set of technologies

Inactive Publication Date: 2003-12-31
NANJING FORESTRY UNIV
View PDF0 Cites 2 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0025] Among the various literatures on plant tissue culture technology at present, there is no report on tissue culture on Chinese sweetgum (Liquidambar formosana) except that the United States has related patent technology reports on liquidambar styraciflua (Liquidambar formosana). Complete technology release

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Chinese sweetgum tissue culture and quick propagation method
  • Chinese sweetgum tissue culture and quick propagation method
  • Chinese sweetgum tissue culture and quick propagation method

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0095] Embodiment 1: adopt Chinese liquidambar 1-year-old seedling tip to carry out tissue culture

[0096] In the last ten days of March, select actively growing branches from the vigorously growing 1-year-old seedlings of Liquidambar chinensis in the field, take the 5cm tops, and disinfect them with 75% ethanol for 30 seconds, 0.1% mercury liter for 2 minutes, and rinse them with sterile water for 4 to 5 times. Finally, 1.0 to 1.5 cm shoot tips were cut as explants.

[0097] The basic medium used for shoot tip growth and bud differentiation and proliferation is WPM and MS medium, and the additional plant hormone combinations are BA 0, 0.1, 0.5, 1.0, 2.0mg / l, NAA 0, 0.05, 0.2mg / l, Remove the phytohormone combination whose BA concentration is lower than NAA, and add TDZ 0.1, 0.01mg / l for WPM culture, the medium contains 3% sucrose, 0.65% agar, the pH before sterilization is adjusted to pH5.8, 121°C high temperature and high pressure Sterilize for 16 minutes, cultivate at a te...

Embodiment 2

[0109] Embodiment 2: adopt the leaf and the petiole that the shoot tip tissue culture of Chinese sweetgum 1-year-old seedling is cultured to carry out tissue culture

[0110]On the differentiated buds of Chinese sweetgum cultivated from the shoot tip, select the young leaves that are unfolding or just unfolding, and cut them off together with the petioles. Under sterile conditions, use a scalpel to separate the leaf from the petiole, leaving 1-2 mm of the end of the petiole on the leaf, then cut the leaf transversely into thin strips of 3-5 mm wide with a scalpel, and place the adaxial side up on the organogenesis On the culture medium, the petioles were cut into small pieces of about 5 mm and placed flat on the organogenesis medium.

[0111] The minimal medium used was WPM and the additional phytohormone combinations were NAA 0, 0.1 mg / l, BA 0.5, 1.0, 2.5, 5.0 mg / l. In addition, TDZ 0.01 mg / l was added alone. The medium contains 3% sucrose and 0.65% agar, and the medium is ...

Embodiment 3

[0115] Embodiment 3: Cultivate Chinese sweetgum test-tube plantlet with seed hypocotyl

[0116] Liquidambar chinensis seeds were washed with running water for 3 hours, sterilized with 75% ethanol for 30 seconds and 0.1% mercuric chloride for 10 minutes, rinsed with sterile water for 4 to 5 times, and inoculated on hormone-free MS medium. After 1 week, the seeds began to germinate, and when the germinated seedlings grew to 1-2 cm, the hypocotyls were removed and cut into 3-5 mm segments as explants. The minimal media for callus induction were MS and MS / 2. The hormones used in the medium are 0.5-3.0 mg / l 2,4-D, 0-2.0 mg / l BA. The hormones used for adventitious bud induction are 0.5-1.0 mg / l ZT, 0.5-1.0 mg / l BA and 0.1 mg / l NAA.

[0117] When the callus was transferred from the callus proliferation medium to the adventitious bud differentiation medium, three pretreatment methods were used:

[0118] 1. Direct transfer without pretreatment, this method is used as a control;

[...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The present invention is characterized by that on the basis of selecting dominant tree its respectively adopts the annual seedling stem apex, leaf blade and petiole, hypocotyl, male rhachis and immature embryo of Chinese sweetgum to make tissue cultivation. Said invention provides a seedling cultivating method for large-scal planting Chinese sweetgum, and its period is short, propagation coefficient is high and cost is low.

Description

1. Technical field [0001] The invention belongs to forest tree tissue culture seedling raising technology in forestry science 2. Background technology [0002] Chinese sweetgum (Liquidambar formosana) is a species of liquidambar formosana in the family Hamamelis family. There are four main species of Liquidambar in modern times, all of which are tall deciduous trees that grow in warm subtropical regions. Among them, the American sweetgum (Liquidambar styraciflua) is widely distributed in the south and southeast of North America, and the high-altitude mountainous areas from Mexico, Central America to the south of Honduras; Styrax (L.orientalis) is a species of Asia Minor, now distributed in the southwest of Turkey There are two species of Chinese sweetgum (Liquidambarformosana) and Liquidambar acalycina distributed in my country. They are widely distributed in my country, from Taiwan in the east, to the border of Sichuan and Tibet in the west, and to Guangdong and Taiwan in t...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
IPC IPC(8): A01H4/00
Inventor 施季森王洪云诸葛强
Owner NANJING FORESTRY UNIV
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com