Enzyme-labeled antibody protection liquid
An enzyme-labeled antibody and protective solution technology, used in instruments, measuring devices, scientific instruments, etc., can solve the problems that the protective solution cannot be stored for a long time and cannot be used, and achieves low microbial utilization, improved shelf life, and is not easy to deteriorate. Effect
Pending Publication Date: 2021-10-29
深圳市计量质量检测研究院(国家高新技术计量站国家数字电子产品质量监督检验中心)
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Problems solved by technology
[0003] The technical problem to be solved by the present invention is: the existing protection solution cannot be stored for a long time, and cannot be applied to ELISA containing immunoglobulin or ELISA with immunoglobulin as the monitoring object
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Embodiment
[0014] Embodiment: This embodiment provides an enzyme-labeled antibody protection solution, which includes the following components: 1 g of sialoglycoprotein (derived from bird’s nest), 2 g of gelatin (derived from bovine), 5 g of Procline 200, 5 g of Triton X-100, Polyvinylpyrrolidone 5 g, 0.1 M pH=7.4 phosphate buffer 1 L. Add sialoglycoprotein and gelatin to phosphate buffer, heat to boiling, stir to dissolve, then cool to room temperature, then add Procline 200, Triton X-100, polyvinylpyrrolidone, and then use a 0.22μm filter membrane to filter and sterilize for sub-packaging .
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The invention relates to the technical field of enzyme-linked immunosorbent assay reagents, and discloses an enzyme-labeled antibody protection liquid, which comprises the following components: sialoglycoprotein (derived from cubilose), gelatin (derived from cattle), Procline 200, Triton X-100 and polyvinylpyrrolidone, wherein the pH value is adjusted to 7.4 by using a 0.1 M phosphate buffer. According to the enzyme-labeled antibody protection liquid, sialoglycoprotein from cubilose is adopted as protection protein, is protein with good water-retaining property, has a good protection effect on antibodies and enzymes, is from saliva of canaries and does not contain immune globulin, so that the problem that an existing enzyme-labeled antibody protection liquid cannot be suitable for enzyme-linked immunosorbent assay containing immune globulin or enzyme-linked immunosorbent assay with the immune globulin as a detection object due to the fact that the existing enzyme-labeled antibody protection liquid contains the immune globulin is solved.
Description
technical field [0001] The invention relates to the technical field of enzyme-linked immunoassay reagents, in particular to an enzyme-labeled antibody protection solution. Background technique [0002] In the production of enzyme-linked immunoassay kits, it is necessary to use protection solutions to protect antibodies and enzyme-labeled antibodies, so that commercial kits have a longer shelf life. The protection period of the current protection solution for the enzyme-labeled antibody is generally 1 year, which cannot meet the requirements of long-term storage. And because it contains immunoglobulins brought in by protein components, such as traditional enzyme-labeled antibody protection solutions containing bovine serum albumin, ovalbumin, milk protein and other proteins, it is inevitable to substitute IgG, IgY and other immunoglobulins, giving some Immunoglobulin Lin Min’s immunoassay experiment brings background value, which makes the ELISA kit not suitable for some spe...
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IPC IPC(8): G01N33/535
CPCG01N33/535
Inventor 张恒张世伟冯荣虎林霖劳翠瑜朱成杰王坤王珍妮
Owner 深圳市计量质量检测研究院(国家高新技术计量站国家数字电子产品质量监督检验中心)
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