Method for screening and identifying shade-tolerant soybean germplasm
An identification method, soybean technology, applied in the cultivation of leguminous plants, resources, instruments, etc., can solve the problems that hinder the development of soybean production, lack of genetic mechanism of soybean shade tolerance, etc.
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Embodiment 1
[0028] The shade-tolerant soybean germplasm screening and identification method specifically comprises the following steps:
[0029] (1) Adopt the potted method to sow 3 soybeans / pot, and 2 health-care and strong seedlings after emergence;
[0030] (2) After 25 days of emergence, it is divided into natural light treatment and shading treatment until maturity;
[0031] Among them, the shading treatment is specifically: using a knitted nylon net with an extinction rate of 40% for shading;
[0032] (3) After the soybeans are mature, measure the number of main stem nodes, branch numbers, internode length, lodging resistance, pod number per plant, 100-grain weight and grain weight per plant of natural light treatment and shading treatment respectively, and calculate The shade tolerance coefficient of each trait index, the membership function value of each comprehensive index, the weight of each comprehensive index and the comprehensive shade tolerance evaluation value of each vari...
Embodiment 2
[0038]The shade-tolerant soybean germplasm screening and identification method specifically comprises the following steps:
[0039] (1) Adopt the potted method to sow 4 soybeans / pot, and 2 health-care and strong seedlings after emergence;
[0040] (2) After 28 days of emergence, it is divided into natural light treatment and shading treatment until maturity;
[0041] Among them, the shading treatment is specifically: using knitted nylon mesh with an extinction rate of 45% for shading;
[0042] (3) After the soybeans are mature, measure the number of main stem nodes, branch numbers, internode length, lodging resistance, pod number per plant, 100-grain weight and grain weight per plant of natural light treatment and shading treatment respectively, and calculate The shade tolerance coefficient of each trait index, the membership function value of each comprehensive index, the weight of each comprehensive index and the comprehensive shade tolerance evaluation value of each variet...
Embodiment 3
[0048] The shade-tolerant soybean germplasm screening and identification method specifically comprises the following steps:
[0049] (1) adopt the pot planting method to sow 5 soybeans / pot, and 2 health-care and strong seedlings after emergence;
[0050] (2) After 30 days of emergence, it is divided into natural light treatment and shading treatment until maturity;
[0051] Among them, the shading treatment is specifically: using a knitted nylon net with an extinction rate of 50% for shading;
[0052] (3) After the soybeans are mature, measure the number of main stem nodes, branch numbers, internode length, lodging resistance, pod number per plant, 100-grain weight and grain weight per plant of natural light treatment and shading treatment respectively, and calculate The shade tolerance coefficient of each trait index, the membership function value of each comprehensive index, the weight of each comprehensive index and the comprehensive shade tolerance evaluation value of eac...
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