Micro-ecological microbial agent composite dietary fiber sea cucumber feed

Abstract

The invention belongs to the technical field of feeds, further relates to a sea cucumber feed, and particularly relates to a micro-ecological microbial agent composite dietary fiber sea cucumber feed as well as preparation and application thereof. The sea cucumber feed is prepared from the following components in parts by weight: 32-51 parts of main materials, 8-14 parts of auxiliary materials, 35-45 parts of sea mud, 0.5-2 parts of dietary fibers and a micro-ecological microbial agent, 600 ml-900 ml of a micro-ecological bacterium agent and 200 ml-300 ml of distilled water are added into every 100 parts of the main material, wherein the micro-ecological microbial inoculant is a complex microbial inoculant composed of saccharomycetes, lactic acid bacteria and bacillus subtilis according to the volume ratio of 1:1:1; and the dietary fibers are one or more of jerusalem artichoke whole flour, wheat aleurone layers and fructo-oligosaccharide. The sea cucumber feed disclosed by the invention has relatively good improvement effects on recovering the diversity of intestinal flora of sea cucumbers, improving the immunity and disease resistance of the sea cucumbers and further improving the survival rate and growth rate of the cultured sea cucumbers.

Description

technical field

[0001] The invention belongs to the technical field of feed, and further relates to a sea cucumber feed, in particular to a microecological bacterial agent compound dietary fiber sea cucumber feed and its preparation and application. Background technique

[0002] Sea cucumber has important nutritional and medicinal value, and plays an important ecological function in the energy cycle of marine matter. With the continuous expansion of the breeding scale, the sea cucumber farming industry is facing severe challenges such as the deterioration of the breeding environment, frequent occurrence of diseases, and drug residues; the intensive aquaculture model will cause serious pollution of the breeding water, and the bait that is put in has more impurities. Unscientific feeding amount, large amount of bait remaining, low utilization rate, gradual deterioration of bottom mud, and deterioration of water quality lead to the proliferation of harmful bacteria and viruses ...

Examples

Embodiment 1

[0045] The preparation method of probiotics compound dietary fiber sea cucumber feed, its operation steps are as follows:

[0046] (1) Bacterial strain expansion cultivation: the required lactic acid bacteria, saccharomycetes, and Bacillus subtilis of the present invention were all purchased from Shandong Dongying Haiyue Aquatic Technology Co., Ltd., and each strain was cultivated in the laboratory to 3 × 10 9 CFU / mL, mix evenly according to the ratio of 1:1:1 by volume, and then get the compound microbial agent.

[0047] Preparation of medium: ①Yeast culture medium: 2 g of glucose, 5 g of yeast extract, 5 g of peptone, 8 g of agar powder, 200 g of potatoes, boiled and filtered juice, dissolved in distilled water to 1000 ml. ②Lactic acid bacteria medium 1000 ml: glucose 20 g, casein peptone 10 g, sodium acetate 7 g, magnesium sulfate 0.2 g, manganese sulfate 0.05 g, potassium dihydrogen phosphate 2 g, disodium citrate 2 g, yeast extract 5 g, Tween 80 ml, agar powder 8 g, dis...

Embodiment 2

[0057] The preparation method of probiotics compound dietary fiber sea cucumber feed, its operation steps are as follows:

[0058] (1) Bacteria expansion culture: cultivate the required lactic acid bacteria, yeast and Bacillus subtilis in the laboratory to 6×10 9 CFU / mL, mix evenly according to the ratio of 1:1:1 by volume, and then get the compound microbial agent.

[0059] (2) Pretreatment of algae: wash sargassum, large leafy vegetables, and hijiki with water, dry or dry until the moisture content is ≦12%, preliminarily crush them with a grinder, and pass through a 50-mesh sieve;

[0060] (3) Preparation of main ingredients: 200 g of sargassum prepared in step (1), 90 g of large leafy vegetables, 40 g parts of hijiki, 60 g of soybean meal, and 30 g parts of sweet potato powder are mixed to obtain coarse powder of main ingredients;

[0061] (4) Adding probiotics: Add the activated and expanded probiotics to the coarse powder of the main ingredient and add distilled water fo...

Embodiment 3

[0067] A kind of preparation method of probiotics compound dietary fiber sea cucumber feed of the present invention, its operating steps are as follows:

[0068] (1) Bacteria expansion culture: cultivate the required lactic acid bacteria, yeast and Bacillus subtilis in the laboratory to 8×10 9 CFU / mL, mix evenly according to the ratio of 1:1:1 by volume, and then get the compound microbial agent.

[0069] (2) Pretreatment of algae: wash sargassum, large leafy vegetables, and hijiki with water, dry or dry until the moisture content is ≦12%, preliminarily crush them with a grinder, and pass through a 50-mesh sieve;

[0070] (3) Preparation of main ingredient meal: mix 250 g of sargassum prepared in step (1), 100 g of large leafy vegetable, 50 g of hijiki, 70 g of soybean meal, and 40 g of sweet potato powder to obtain the main ingredient coarse powder. pink;

[0071] (4) Adding probiotics: Add the activated and expanded probiotics to the coarse powder of the main ingredient an...

technical field

[0001] The invention belongs to the technical field of feed, and further relates to a sea cucumber feed, in particular to a microecological bacterial agent compound dietary fiber sea cucumber feed and its preparation and application. Background technique

[0002] Sea cucumber has important nutritional and medicinal value, and plays an important ecological function in the energy cycle of marine matter. With the continuous expansion of the breeding scale, the sea cucumber farming industry is facing severe challenges such as the deterioration of the breeding environment, frequent occurrence of diseases, and drug residues; the intensive aquaculture model will cause serious pollution of the breeding water, and the bait that is put in has more impurities. Unscientific feeding amount, large amount of bait remaining, low utilization rate, gradual deterioration of bottom mud, and deterioration of water quality lead to the proliferation of harmful bacteria and viruses ...

Examples

Embodiment 1

[0045] The preparation method of probiotics compound dietary fiber sea cucumber feed, its operation steps are as follows:

[0046] (1) Bacterial strain expansion cultivation: the required lactic acid bacteria, saccharomycetes, and Bacillus subtilis of the present invention were all purchased from Shandong Dongying Haiyue Aquatic Technology Co., Ltd., and each strain was cultivated in the laboratory to 3 × 10 9 CFU / mL, mix evenly according to the ratio of 1:1:1 by volume, and then get the compound microbial agent.

[0047] Preparation of medium: ①Yeast culture medium: 2 g of glucose, 5 g of yeast extract, 5 g of peptone, 8 g of agar powder, 200 g of potatoes, boiled and filtered juice, dissolved in distilled water to 1000 ml. ②Lactic acid bacteria medium 1000 ml: glucose 20 g, casein peptone 10 g, sodium acetate 7 g, magnesium sulfate 0.2 g, manganese sulfate 0.05 g, potassium dihydrogen phosphate 2 g, disodium citrate 2 g, yeast extract 5 g, Tween 80 ml, agar powder 8 g, dis...

Embodiment 2

[0057] The preparation method of probiotics compound dietary fiber sea cucumber feed, its operation steps are as follows:

[0058] (1) Bacteria expansion culture: cultivate the required lactic acid bacteria, yeast and Bacillus subtilis in the laboratory to 6×10 9 CFU / mL, mix evenly according to the ratio of 1:1:1 by volume, and then get the compound microbial agent.

[0059] (2) Pretreatment of algae: wash sargassum, large leafy vegetables, and hijiki with water, dry or dry until the moisture content is ≦12%, preliminarily crush them with a grinder, and pass through a 50-mesh sieve;

[0060] (3) Preparation of main ingredients: 200 g of sargassum prepared in step (1), 90 g of large leafy vegetables, 40 g parts of hijiki, 60 g of soybean meal, and 30 g parts of sweet potato powder are mixed to obtain coarse powder of main ingredients;

[0061] (4) Adding probiotics: Add the activated and expanded probiotics to the coarse powder of the main ingredient and add distilled water fo...

Embodiment 3

[0067] A kind of preparation method of probiotics compound dietary fiber sea cucumber feed of the present invention, its operating steps are as follows:

[0068] (1) Bacteria expansion culture: cultivate the required lactic acid bacteria, yeast and Bacillus subtilis in the laboratory to 8×10 9 CFU / mL, mix evenly according to the ratio of 1:1:1 by volume, and then get the compound microbial agent.

[0069] (2) Pretreatment of algae: wash sargassum, large leafy vegetables, and hijiki with water, dry or dry until the moisture content is ≦12%, preliminarily crush them with a grinder, and pass through a 50-mesh sieve;

[0070] (3) Preparation of main ingredient meal: mix 250 g of sargassum prepared in step (1), 100 g of large leafy vegetable, 50 g of hijiki, 70 g of soybean meal, and 40 g of sweet potato powder to obtain the main ingredient coarse powder. pink;

[0071] (4) Adding probiotics: Add the activated and expanded probiotics to the coarse powder of the main ingredient an...

Images