Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Culture container for culturing epithelial cells and use thereof

An epithelial cell and culture container technology, applied in cell culture active agent, culture process, tissue culture and other directions, can solve the problems of artificial culture of intestinal bacteria and inability to clone.

Pending Publication Date: 2021-12-24
KEIO UNIV
View PDF5 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, many gut bacteria remain uncultured and cannot be cloned
Such technical constraints represent a bottleneck in the understanding of gut bacteria in medical biology and in the production of commercial gut bacteria

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Culture container for culturing epithelial cells and use thereof
  • Culture container for culturing epithelial cells and use thereof
  • Culture container for culturing epithelial cells and use thereof

Examples

Experimental program
Comparison scheme
Effect test

experiment example 1

[0166] (culture of epithelial cells)

[0167] use figure 2 (a)~ figure 2 The culture container for epithelial cell culture shown in the photograph in (c) is used for culturing epithelial cells. As the upper container, a commercially available insert cell culture dish (trade name "ThinCert", Greiner Bio-One) or a cell culture tank (trade name "Transwell", Corning Corp.) was used. In addition, the cover member is self-made. As the material of the cover member, butyl rubber was used. The oxygen permeability coefficient of butyl rubber is 0.99×10 -8 cm 3 cm / (cm 2 · seconds · air pressure). In addition, as the lower container, a commercially available 12-well plate or 48-well plate was used.

[0168]

[0169] As a cell culture medium, a medium was prepared as follows: Human recombinant R-sponpin was added to a commercially available Advanced DMEM / F-12 medium (manufactured by Thermo Fisher SCIENTIFIC) at a final concentration of 1 μg / mL 1 (manufactured by R&D systems), ...

experiment example 2

[0182] (measurement of oxygen concentration)

[0183] The same operation as in Experimental Example 1, with figure 2 (a)~ figure 2 Intestinal epithelial cells are cultured in the culture vessel for culturing epithelial cells shown in the photograph in (c). The interior of the upper vessel is maintained at anaerobic conditions and the interior of the lower vessel is maintained at aerobic conditions. In addition, for comparison, the following samples were also prepared, that is, the sample that was subjected to the same operation as Experimental Example 1 except that the epithelial cells were not seeded, and the sample that did not fit the lid member to the opening of the upper container. samples of cultured epithelial cells.

[0184] Next, 3 days after the epithelial cells were inoculated into the upper container, the dissolved oxygen concentration in the cell culture medium in the upper container and the dissolved oxygen concentration in the cell culture medium in the low...

experiment example 3

[0189] (2D organoid research1)

[0190] use figure 2 (a)~ figure 2 In the culture vessel for culturing epithelial cells shown in the photograph in (c), intestinal epithelial cells were cultured in a state where the upper vessel was kept under anaerobic conditions, and whether epithelial stem cells could be maintained or differentiated cells could be maintained. Research.

[0191] First, in the same manner as in Experimental Example 1, a layer of fused 2D organoids was formed on the surface of the upper container. Next, the culture medium in the upper vessel was replaced in the anaerobic chamber. Two sets of the same culture containers for epithelial cell culture were prepared, and in one culture container for epithelial cell culture, the lid member was fitted to the opening of the upper container, while in the other culture container for epithelial cell culture, the lid member was not used. Fits into the opening of the upper container. These cells were incubated at 37°C...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

PropertyMeasurementUnit
concentrationaaaaaaaaaa
Login to View More

Abstract

A culture container for culturing epithelial cells that comprises an upper container, a lid member air tightly fitting into the opening of the upper container, and a lower container housing the upper container and a cell culture medium therein, wherein; at least a part of an area of the upper container, said area being in contact with the cell culture medium, is formed of a membrane that is permeable to at least some components of the cell culture medium and impermeable to cells; and the material of the lid member has an oxygen permeation coefficient of not more than 1.0x10<-6> cm<3> cm / (cm<2> s.Pa).

Description

technical field [0001] The invention relates to a culture container for epithelial cell culture and its use. More specifically, the present invention relates to a culture vessel for culturing epithelial cells, a method for culturing epithelial cells, and a co-culture of epithelial cells and anaerobic bacteria. This application claims priority based on Japanese Patent Application No. 2019-096905 filed in Japan on May 23, 2019, the contents of which are incorporated herein by reference. Background technique [0002] With advances in sequence technology in recent years, systematic classification of molecular genetics of intestinal bacteria is advancing. In addition, functional analysis of gut bacteria can be performed by inoculating germ-free mice with gut bacteria. However, many gut bacteria remain uncultured and cannot be cloned. Such technical constraints represent a bottleneck in the understanding of gut bacteria in medical biology and in the production of commercial gut...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Applications(China)
IPC IPC(8): C12M1/00C12N5/071C12N1/20
CPCC12N5/0679C12N2501/115C12N2501/11C12N2501/15C12N2501/155C12M21/08C12M25/04C12M23/38C12M23/24C12N2501/105C12N2501/415C12N2502/70C12M23/36C12M25/02C12M29/04C12N2500/02
Inventor 佐藤俊朗佐佐木伸雄
Owner KEIO UNIV
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com