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Primer group, kit and identification method for identifying sex of macrobrachium rosenbergii and application

A technology of Macrobrachium rosenbergii and identification method, which is applied in the field of molecular identification of sex chromosomes of Macrobrachium rosenbergii, and can solve problems such as difficult typing and small sex chromosomes

Pending Publication Date: 2022-01-04
SHANGHAI OCEAN UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, Macrobrachium rosenbergii, as a decapod animal, has a large number of chromosomes and small sex chromosomes, making it difficult to type

Method used

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  • Primer group, kit and identification method for identifying sex of macrobrachium rosenbergii and application
  • Primer group, kit and identification method for identifying sex of macrobrachium rosenbergii and application
  • Primer group, kit and identification method for identifying sex of macrobrachium rosenbergii and application

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Embodiment Construction

[0027] In order to make the purpose, technical solution and advantages of the present application clearer, the present application will be further described in detail below in conjunction with the embodiments. It should be understood that the specific embodiments described here are only used to explain the present application, and are not intended to limit the present application. The reagents or instruments involved in the following examples can be obtained from commercial glue unless otherwise specified; the experimental methods are conventional methods unless otherwise specified.

[0028] 1. Macrobrachium rosenbergii and tissue materials

[0029] Eight female Macrobrachium rosenbergii were purchased from the orchard farmer's market, and the muscle tissue was immediately frozen with liquid nitrogen and stored in a -80°C refrigerator for later use.

[0030] Male and female phenotype identification of Macrobrachium rosenbergii: Take the gonad tissue of Macrobrachium rosenberg...

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Abstract

The invention discloses a primer group for PCR (Polymerase Chain Reaction) amplification of a macrobrachium rosenbergii sex chromosome fragment. The primer group comprises at least one of a first primer pair as shown in SEQ ID NO. 1-2, a second primer pair as shown in SEQ ID NO. 3-4 and a third primer pair as shown in SEQ ID NO. 5-6. The primer group can be used for effectively amplifying specific fragments, the sex of the macrobrachium rosenbergii can be effectively identified according to the fragments, even, male, female and superfemale genotypes can be distinguished, and help is provided for cross breeding and genetic typing of the macrobrachium rosenbergii.

Description

technical field [0001] The present application relates to the technical field of molecular identification of sex chromosomes of Macrobrachium rosenbergii, and in particular to a primer set, a kit, a identification method and an application for identifying the sex of Macrobrachium rosenbergii. Background technique [0002] Macrobrachium rosenbergii (Macrobrachium rosenbergii) is a large freshwater prawn belonging to the genus Macrobrachium rosenbergii in the family Arthropoda. As an important economical shrimp, it is widely distributed in South and Southeast Asia and the Western Pacific. There are significant differences in growth and development rates between male and female individuals. After sexual maturity, male individuals are significantly larger than female individuals, and the economic effect is higher. Breeding an all-male population of Macrobrachium rosenbergii is an effective strategy to increase yield. Moreover, the yield of monosexual breeding is higher than th...

Claims

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Application Information

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IPC IPC(8): C12Q1/6888C12Q1/6879C12Q1/686C12Q1/6851C12N15/11
CPCC12Q1/6888C12Q1/6879C12Q1/686C12Q1/6851C12Q2600/124C12Q2565/125C12Q2531/113
Inventor 刘雪邱高峰马克异
Owner SHANGHAI OCEAN UNIV
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