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Signal path PCR chip data analysis system, local computer equipment and cloud platform

A data analysis system and signal path technology, applied in the field of local computer equipment and cloud platform, signal path PCR chip data analysis system, can solve problems such as weak programming foundation, complicated data analysis method and process, and difficult data analysis by production personnel.

Pending Publication Date: 2022-03-25
SHANGHAI BIOCHIP
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  • Claims
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Problems solved by technology

[0005] In view of the shortcomings of the prior art described above, the purpose of the present invention is to provide a signal path PCR chip data analysis system, local computer equipment and cloud platform, for solving the existing data analysis method for PCR chip in the prior art The process is complex, especially the programming foundation has certain requirements, so it is difficult for scientific research, testing, and production personnel with weak or even zero programming foundation to conduct data analysis or obtain analysis results easily.

Method used

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  • Signal path PCR chip data analysis system, local computer equipment and cloud platform
  • Signal path PCR chip data analysis system, local computer equipment and cloud platform
  • Signal path PCR chip data analysis system, local computer equipment and cloud platform

Examples

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Embodiment 1

[0071] Example 1: Signal pathway PCR chip data analysis system.

[0072] Before using the system for analysis, demo data should be imported into the signal pathway PCR chip data analysis system and corresponding parameters should be set;

[0073] Among them, demo data includes: original Ct file, gene layout file, grouping file and group comparison file; among them,

[0074] The original Ct file, its partial contents are as follows figure 2 As shown; where, the column names (such as control-1, control-2, control-3) are the sample names, the row names (such as A1, A2, A3) are the positions of the 96-well plate, and the values ​​are the original Ct values. H1-5 are housekeeping genes; H6-12 are yin ginseng, yang ginseng and quality control.

[0075] A gene layout file, part of which is image 3 Shown; PCA Array Gene Layout & Gene Annotation, where Position, Symbol, and GeneID are the 96-well plate position, gene name, and Entrez ID respectively.

[0076] Group files such as ...

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Abstract

The invention provides a signal path PCR chip data analysis system, local computer equipment and a cloud platform. Through an original Ct value file, a chip layout file, a grouping file and a group comparison file of a signal path PCR chip and set personalized parameters, the data of the PCR chip is automatically subjected to standardization, statistical analysis, visualization and functional analysis, and a report result is finally generated. Statistical analysis and visualization results can be directly used for article publishing, biomedical detection reports and the like.

Description

technical field [0001] The invention relates to the field of biotechnology, in particular to a signal path PCR chip data analysis system, local computer equipment and a cloud platform. Background technique [0002] The signaling pathway PCR chip uses the detection method of fluorescent quantitative PCR, which can detect the expression level of important related genes of a specified signaling pathway on several 96-well plates. Each 96-well plate of each pathway product mainly contains more than 80 major related genes, several internal references, positive controls, negative controls, etc. [0003] Researchers can study a large number of genes at the same time, not only for the study of some signal pathways of special interest, but also as a method for verifying the results of chips and high-throughput sequencing. At the same time, the data analysis charts obtained from the experiment can be directly used for publication article. PCR chips are widely used in the fields of li...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G16B20/20G16B50/30
CPCG16B20/20G16B50/30
Inventor 产天龙韩峻松徐祎春袁箐陈超毛履祥
Owner SHANGHAI BIOCHIP
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