Chip and kit for detecting novel coronavirus nucleic acid as well as preparation method and application of chip and kit

A coronavirus and chip technology, applied in the field of chips for detecting new coronavirus nucleic acid, can solve problems such as prolonging the detection time, and achieve the effects of cheap preparation materials, simple equipment and less sample demand

Pending Publication Date: 2022-04-05
SHANDONG UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, the extraction and amplification of nucleic acid are essential in the detection process, thus prolonging the overall detection time

Method used

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  • Chip and kit for detecting novel coronavirus nucleic acid as well as preparation method and application of chip and kit
  • Chip and kit for detecting novel coronavirus nucleic acid as well as preparation method and application of chip and kit
  • Chip and kit for detecting novel coronavirus nucleic acid as well as preparation method and application of chip and kit

Examples

Experimental program
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preparation example Construction

[0051] The preparation method of the above-mentioned chip comprises the following steps:

[0052] Step 1, preparation of functionalized substrate 1 , preparation of channel layer 2 and preparation of valve control layer 3 .

[0053] 1. The preparation of the functionalized substrate 1 includes the following processes:

[0054] (1) Cleaning the glass slide: streak the glass slide to divide the growth area of ​​GO and PLL; then put the glass slide into the piranha solution and boil it, then use deionized water to ultrasonicate the glass slide cleaning;

[0055] (2) Plasma treatment of the surface of the glass slide: the cleaned glass slide is treated with a plasma cleaning machine for surface hydroxylation, which facilitates the combination of APTES and the functionalized substrate;

[0056] (3) Soak slides in APTES: Soak part of the Plasma-treated slides (3 / 5 width) in APTES solution, take them out after 20 minutes, clean the slides ultrasonically with deionized water, and dr...

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Abstract

The invention discloses a chip and a kit for detecting novel coronavirus nucleic acid as well as a preparation method and application, the chip comprises a functional substrate, a runner layer and a valve control layer which are sequentially arranged from bottom to top, and the functional substrate is divided into a GO nucleic acid capture area and a PLL detection area; a capture probe E of an SARS-CoV-2 virus E section is fixed in a region, positioned in the sample injection flow channel, of the functionalized substrate; the kit comprises the chip, confining liquid, cleaning liquid, a cracking reagent, a negative reference substance and a positive reference substance. When the kit is adopted for detection, nucleic acid extraction and amplification are not needed, the detection sensitivity is high, the sample demand quantity is small, the detection process is simple, and the detection time is short. And preparation materials are cheap, and devices are simple.

Description

technical field [0001] The invention relates to the field of molecular biology detection, in particular to a chip, kit, preparation method and application for detecting novel coronavirus nucleic acid. Background technique [0002] The pneumonia epidemic caused by the novel coronavirus (SARS-CoV-2) poses a huge threat to public health in the world. Compared with most previous epidemic infectious diseases, SARS-CoV-2 is more contagious. In order to detect viruses faster and suppress the spread of viruses, the development of a fast, accurate and sensitive molecular screening technology is a key task that needs to be solved urgently. Based on the fact that SARS-CoV-2 is a beta-coronavirus with a single-stranded RNA genome and contains S protein, E protein, M protein, and N protein, nucleic acid and antibody detection methods are used to screen for SARS-CoV -2. Antibody detection based on the above proteins is mainly used to detect SARS-specific antibodies (such as IgG, IgM and...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12M1/34C12M1/00C12Q1/70C12Q1/6851C12Q1/6806
Inventor 韩琳褚玉金张宇
Owner SHANDONG UNIV
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