T-grade composite microbial inoculant as well as preparation method and application thereof

A composite bacterial agent and microorganism technology, applied in the field of high-efficiency lignocellulose composite bacterial agent preparation, can solve the problems of low degradation efficiency and difficult degradation of lignocellulose waste, achieve efficient degradation of lignocellulose, avoid competitive repulsion, The effect of promoting the production of microbial enzymes

Active Publication Date: 2022-04-12
NORTHEAST AGRICULTURAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, there is no relevant research on how to use microorganisms to solve the problems of refractory degradation and low degradation efficiency of lignocellulosic waste in the composting process.

Method used

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  • T-grade composite microbial inoculant as well as preparation method and application thereof
  • T-grade composite microbial inoculant as well as preparation method and application thereof
  • T-grade composite microbial inoculant as well as preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0029] Four kinds of mesophilic characteristic bacteria numbered Z1, B4, H41, and D3, three kinds of high-temperature-resistant characteristic bacteria numbered D2, D8, and A1, and normal-temperature characteristic bacteria numbered B4, H61, and H41 (wherein B4 and H41 two kinds of bacteria have both mesophilic and normal temperature characteristics) were cultured in LB medium for 48 hours under the conditions of 18°C, 32°C, 45°C and 58°C respectively, so that the effective number of viable bacteria of each strain was 1×10 7 ~1×10 9 CFU / mL, then according to Z1*D3*D2*A1*B4, Z1*D2*H61, Z1*H41*A1*D3, Z1*B4*H41*D3, D3*H41*A1, A1*B4, D2* D3*D8*A1*B4, D2*H61*A1, and H61*A1*B4 were inoculated into LB medium in the grouping (each group was mixed in equal volume), and cultured at 20°C for 1 day, 35 Cultivate for 1 day at 45°C, 1 day at 45°C, 2 days at 50°C, 2 days at 55°C, and 1 day at 60°C, so that the total effective number of viable bacteria is 1×10 7 ~1×10 9 CFU / mL, subculture ...

Embodiment 2

[0032] The difference from Example 1 is that psychrophilic bacillus is added as a cold-resistant functional strain on the basis of each group of composite strains in Example 1, and other steps are the same as in Example 1.

[0033] The results show that the method of the present invention can quickly degrade lignocellulose in a short period of time, and the lignocellulose degradation rate of the prepared composite bacterial agent is obviously improved by 20% compared with that of single bacteria, the cellulase activity is improved by 25%, and the hemicellulase activity is improved by 20%. %, lignin enzyme activity increased by 20%.

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Abstract

The invention provides a T-grade microbial complex microbial inoculant as well as a preparation method and application thereof, and belongs to the technical field of preparation of efficient lignocellulose complex microbial inoculants. The preparation method of the T-grade composite microbial inoculant provided by the invention comprises the following steps: mixing microbial culture solutions with the effective viable count of 1 * 10 < 7 >-1 * 10 < 9 > CFU/mL and different temperature characteristics in an isovolumetric manner, culturing according to a gradient heating manner to enable the total effective viable count to be 1 * 10 < 7 >-1 * 10 < 9 > CFU/mL, subculturing, and carrying out fermentation culture on the composite microbial inoculant obtained by subculturing according to a composting temperature to obtain the T-grade composite microbial inoculant. And screening to obtain the complex microbial inoculant. The complex microbial inoculant prepared by the preparation method disclosed by the invention has the effect of efficiently degrading lignocellulose, ensures that dominant strains exert maximum activity and generate degradation capacity at corresponding composting temperatures, and ensures that the effects generated by the dominant strains at different temperatures are like T-level when a rocket is ignited, so that not only is the competitive repulsive interaction among microorganisms avoided, but also the effect of degrading the lignocellulose is achieved. And organic components in each period of composting can be rapidly degraded.

Description

technical field [0001] The invention belongs to the technical field of preparation of high-efficiency lignocellulose composite bacterial agent, and in particular relates to a T-grade microbial composite bacterial agent and its preparation method and application. Background technique [0002] With the growth of population and the development of social economy, the living standards of our people have been continuously improved, and the output of agricultural waste has also increased significantly. Improper disposal of agricultural waste will not only cause loss of resources, but also have a great impact on the environment. Aerobic fermentation is the main way to deal with the resource utilization of agricultural waste, but due to the problems of weak microbial activity and low microbial enzyme production, it seriously affects the degradation and high-quality resource utilization of agricultural waste. [0003] Agricultural waste generally contains high lignocellulosic biomass...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C12N1/14C05F17/20C12R1/07C12R1/125C12R1/68C12R1/82C12R1/01
CPCY02W30/40
Inventor 魏自民吴迪贾立明吴俊秋赵昕宇党秋玲赵越曲凤婷郑广仁
Owner NORTHEAST AGRICULTURAL UNIVERSITY
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