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Method for high-density culture of alkanet endophyte

A technology of high-density cultivation and comfrey root, which is applied in the biological field to achieve the effects of short time-consuming, easy operation and improved cultivation effect

Active Publication Date: 2022-05-10
南京合谷生命生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0004]However, comfrey root endophytes only exist in the plant comfrey root, and the link from natural planting comfrey to industrial production application of comfrey root endophyte is still little known , only large-scale high-density cultivation of the endophytic bacteria of the root of the plant can save time and effort to obtain the active ingredients of the root of the plant, which can be better used in industrial production

Method used

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  • Method for high-density culture of alkanet endophyte
  • Method for high-density culture of alkanet endophyte
  • Method for high-density culture of alkanet endophyte

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Effect test

Embodiment 1

[0064] The culture method of embodiment 1 high-density comfrey root endophyte

[0065] The specific cultivation method is as follows:

[0066] (2) Activation culture: first add the comfrey root powder into the inoculation medium in an amount of 2% by weight, and then inoculate the 100mL comfrey root powder containing comfrey root endophyte strain with the inoculation amount The culture medium was placed in a shaker for shaking culture at a temperature of 37°C and a rotation speed of 225rpm, and cultured for 24 hours to obtain a co-culture solution of endophytic bacteria of comfrey root;

[0067] (3) Fermentation culture: first add the comfrey root powder into the fermentation medium in an amount of 5% by weight, then inoculate the above-mentioned comfrey root endophyte co-culture solution in 5L of fermentation medium containing the comfrey root powder , and then ferment in stages, first at a rotation speed of 300rpm and a sterile air ventilation rate of 2m 3 / h, pH value 5.5...

Embodiment 2

[0068] The culture method of embodiment 2 high-density comfrey root endophytes

[0069] The cultivation process of embodiment 2 is exactly the same as that of embodiment 1, the difference is: in embodiment 2, comfrey root powder is added in the inoculation medium in the addition amount that is 6% by weight; The OD600 value result of the end point of fermentation of record is shown in the table 5.

Embodiment 3

[0070] The culture method of embodiment 3 high-density comfrey root endophytes

[0071] The cultivation process of embodiment 3 is exactly the same as that of embodiment 1, and the difference is that in embodiment 3, comfrey root powder is added to the inoculation medium in an amount of 7% by weight; the recorded fermentation end point OD600 value results are shown in the table 5.

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Abstract

The invention discloses a method for high-density culture of alkanet endophyte, which comprises the following steps: pretreating alkanet to obtain alkanet powder, inoculating alkanet endophyte strains into an inoculation culture medium containing the alkanet powder, and culturing to obtain alkanet endophyte co-culture solution, inoculating the alkanet endophyte co-culture solution into a fermentation culture medium containing alkanet powder, carrying out aerobic fermentation culture in two stages, adding a supplementary culture medium in a fed-batch manner in the second-stage fermentation, and obtaining the high-density alkanet endophyte after the fermentation is finished. The culture method is simple, convenient, time-saving, labor-saving, green and environment-friendly, the alkanet root powder is added while the alkanet root endophyte is cultured, so that the alkanet root endophyte is massively propagated in a short time, and the culture method is suitable for large-scale production and application.

Description

technical field [0001] The invention relates to a method for high-density cultivating endophytic fungus of comfrey roots, which belongs to the field of biotechnology. Background technique [0002] Comfrey root, is the dried root of Xinjiang Burgeonia, Pseudocomfrey or Yunnan Burgeon, a perennial herb of the Boraginaceae family. Its species are mainly divided into soft Burgeon root and hard Burgeon root, and the soft Burgeon root is irregular and long cylindrical , multi-twisted, mainly including Inner Mongolian Comfrey; hard comfrey roots are conical or cylindrical, twisted, mainly including Laotiao Comfrey, Guan Comfrey, Changde Comfrey, etc. Comfrey root has the effects of clearing away heat and cooling blood, clearing rash, laxative, anti-tumor, anti-inflammation, anti-pathogenic microorganisms, analgesic and sedative. At present, the source of comfrey roots is the plant comfrey, but the planting period of comfrey is long, occupies a large area of ​​land, is greatly affe...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/00
CPCC12N1/00Y02W30/40
Inventor 华夏丁叶李亚
Owner 南京合谷生命生物科技有限公司
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