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Detection kit for breast cancer assisted immunotherapy target gene MARCHF1 and application

A technology for immunotherapy and breast cancer, applied in DNA/RNA fragments, measuring devices, recombinant DNA technology, etc., can solve problems such as side effects and low treatment response rate

Inactive Publication Date: 2022-05-10
JIANGSU VOCATIONAL COLLEGE OF MEDICINE
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Immunotherapy is a promising new way to treat breast cancer, but there are problems such as low response rate and side effects. Therefore, the development of new targets for adjuvant immunotherapy is of great significance for clinical tumor immunotherapy

Method used

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  • Detection kit for breast cancer assisted immunotherapy target gene MARCHF1 and application
  • Detection kit for breast cancer assisted immunotherapy target gene MARCHF1 and application
  • Detection kit for breast cancer assisted immunotherapy target gene MARCHF1 and application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0028] This example is used to illustrate that the expression of the target gene MARCHF1 in breast cancer is significantly up-regulated.

[0029] Analysis of MARCHF1 expression in breast cancer:

[0030] 1. Data download and arrangement:

[0031] The UCSC XENA website (https: / / xenabrowser.net / datapages / ) was used to download the TCGA breast cancer expression profile data and clinical data, and the MARCHF1 expression profile data were transformed by log2 for comparison between groups.

[0032] 2. Analysis of the expression difference of MARCHF1 in breast cancer

[0033] The R package was used to analyze the difference of MARCHF1 mRNA level between breast cancer tissue and normal tissue, and the ggplot2 package was used for visualization, such as figure 1 shown.

[0034] figure 1 Expression changes of the target gene MARCHF1 in breast cancer are shown. (A) The results of unpaired samples showed that the expression of MARCHF1 was significantly up-regulated in breast cancer t...

Embodiment 2

[0036] This example is used to illustrate that the expression of MARCHF1 in breast cancer is significantly positively correlated with the indicators of tumor immune microenvironment.

[0037] Analysis of correlation between MARCHF1 expression and tumor immune microenvironment in breast cancer:

[0038] The R package ESTIMATE was used to calculate the StromalScore, ImmuneScore and ESTIMATE scores of each breast cancer patient, and the corr.test function of the R package psych was used to calculate the Pearson correlation coefficient between MARCHF1 expression and immune infiltration score. As shown in Table 1.

[0039] Table 1 shows the correlation between MARCHF1 expression and tumor immune microenvironment in breast cancer. MARCHF1 expression was significantly positively correlated with ESTIMATEScore, StromalScore and ImmuneScore.

[0040] Table 1

[0041]

Embodiment 3

[0043] This example is used to verify that the expression of MARCHF1 in breast cancer is positively correlated with the infiltration of immune cells.

[0044] Correlation analysis between MARCHF1 expression and immune cell infiltration in breast cancer:

[0045] The R software package GSVA package immune infiltration algorithm ssGSEA, psych's corr.test function was used to calculate the Pearson correlation coefficient between MARCHF1 and immune cell infiltration score, and the ggplot2 package was used for visualization. like figure 2 shown.

[0046] figure 2 The correlation of MARCHF1 expression with immune cell infiltration in breast cancer is shown. MARCHF1 expression was significantly positively correlated with immune cell infiltration.

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Abstract

The invention belongs to the technical field of biology, and relates to a breast cancer assisted immunotherapy target gene MARCHF1 detection kit and application. The research proves that the MARCHF1 expression in breast cancer is obviously increased. The MARCHF1 expression level is in positive correlation with immune microenvironment indexes StromalScore, ImmuneScore and ESTIMATEScore, is in positive correlation with infiltration of various immune cells, and is in positive correlation with gene expression of an immune checkpoint, and the MARCHF1 detection kit is designed, and the MARCHF1 expression level is in positive correlation with immune microenvironment indexes StromalScore, ImmuneScore and ESTIMATEScore.

Description

technical field [0001] The invention belongs to the field of biotechnology, and in particular relates to the application of a reagent for detecting the expression level of MARCHF1 in the preparation of preparations for breast cancer detection and / or adjuvant immunotherapy, and a method for detecting the target gene MARCHF1 of breast cancer adjuvant immunotherapy Kits and applications. Background technique [0002] Breast cancer (Breast cancer, BRCA) is one of the most common malignant tumors in women worldwide, and its incidence rate and mortality rate rank first among all types of cancer. Although the incidence of breast cancer in my country is relatively low, the population base is large, the overall number of patients is large, the incidence rate is increasing year by year, and the age of onset is younger. Immunotherapy is a promising new way to treat breast cancer, but there are problems such as low response rate and side effects. Therefore, the development of new targe...

Claims

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Application Information

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IPC IPC(8): C12Q1/6886G01N33/574G01N33/573C12N15/11A61P35/00A61K45/00
CPCC12Q1/6886G01N33/57415G01N33/57484G01N33/573A61K45/00A61P35/00G01N2333/9108
Inventor 杜欣娜周颖婷李彤彤胡明
Owner JIANGSU VOCATIONAL COLLEGE OF MEDICINE
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