Method for promoting dermal papilla cells to induce hair regeneration through HHORSCs exosome

Abstract

The invention provides a method for promoting dermal papilla cells to induce hair regeneration through HHORSCs exosomes. The method for promoting dermal papilla cells to induce hair regeneration through HHORSCs exosomes comprises the following steps that S1, human dermal papilla cells and human hair follicle outer root sheath cells are cultured and separated from a human scalp sample; s2, evaluating a specific marker by using flow cytometry and an immunocytochemistry technology; s3, separating blood platelets from the human hair follicle outer root sheath cells and the human umbilical cord blood by using an ultracentrifugation technology, and further separating exosomes; s4, detecting a specific marker of the exosome by using a western blot method; s5, analyzing the particle size and distribution of the exosome through a nano-particle tracking analysis technology; and S6, evaluating the influence of exosomes with different concentrations on the regeneration capacity by using different methods such as a proliferation test and a migration test. The method for promoting dermal papilla cells to induce hair regeneration by using the HHORSCs exosome has the advantages of improving alopecia and realizing hair regeneration.

Description

Technical field [0001] The present invention relates to the field of hair regeneration technology, in particular to a method of inducing hair regeneration by exosomes of HHORSCs to promote dermal papillary cells. Background [0002] The problem of hair loss has attracted much attention, but the current treatment of hair loss is expensive and ineffective, from invasive surgery to chemotherapy can not produce the expected results, it is difficult to fundamentally solve the problem of hair loss. At present, there are two main drugs for the treatment of hair loss, namely minoxidil and finasteride, but the two have great limitations in the treatment process, and patients must continue to take it in order to maintain hair growth, strong dependence on drugs, and certain side effects. [0003] Common treatments for hair loss include herbal extracts, platelet-rich plasma, fat-derived stem cells, and hair transplants, but none of these methods produce satisfactory results. [0004] Therefo...

AI Technical Summary

Problems solved by technology

[0002] The problem of hair loss has attracted much attention, but the current treatment methods for hair loss are expensive and ineffective, ranging from invasive surgery to chemotherapy, which cannot produce the expected results, and it is difficult to fundamentally solve the problem of hair loss

At present, there are mainly two kinds of drugs for the treatment of hair loss, namely minoxidil and finasteride, but the two have great limitations in the course of treatment. Patients must continue to take them in order to maintain hair growth. The dependence on drugs Very strong, with certain side effects

[0003] Common treatments for hair loss include herbal extracts, platelet-rich plasma, adipose-derived stem cells, and hair transplantation, etc., however, none of these methods yield satisfactory results

Images