In vitro maturing culture method for human immature ovum

An in vitro mature culture and immature technology, applied in the field of human assisted reproduction, can solve the problems that IVM culture is not as successful as cocIMOs, no advantages in IVM promotion, no special technology or research report, etc., and achieve IVM auxiliary effect. Medical safety is more guaranteed, and the effect of synchronous demand improvement

Inactive Publication Date: 2006-07-12
ZHEJIANG UNIV
View PDF0 Cites 8 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0009] 1), the successful (maturity) rate of pure IVM culture and the subsequent developmental ability of corresponding mature eggs are not as good as the corresponding indicators of cocIMOs [see reference 6, 7]
[0010] 2) MFF and feeder cells used for coIVM are mostly of allogeneic or heterogeneous origin, and there is a corresponding risk of disease transmission and / or cross-infection between allogeneic or heterogeneous [see reference 1]
[0011] 3) There are only two special research reports on autologous ovarian granulosa cells assisting human ccdIMOs CoIVM, neither of which showed advantages in IVM promotion [see reference 2, 20]
[0012] 4), no one autoMFF participated in ccdIMOs IVM special technology or research report
[0013] 5) There is no special technology or research report on the synergy between autoCCs and autoMFF for ccdIMOs coIVM

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • In vitro maturing culture method for human immature ovum
  • In vitro maturing culture method for human immature ovum
  • In vitro maturing culture method for human immature ovum

Examples

Experimental program
Comparison scheme
Effect test

Embodiment Construction

[0077] 1. Prepare standard IVM culture medium:

[0078] The standard IVM medium consists of basal medium, 10 (v / v)% human serum substitute, 0.075IU / ml human follicle-stimulating hormone (FSH) and 0.1IU / mL human chorionic gonadotropin (HCG). In the incubator (37°C, 5% CO 2 , saturated humidity) pre-warmed and equilibrated for at least 4 hours before use.

[0079] The basal culture medium can be selected from the basal culture medium of the MediCult IVM system (Cat#: 82214010, MediCult Company, Denmark) or the HTF culture medium of Irvine Scientific Company (Cat#: 90125, Irvine Scientific Company, USA).

[0080] The 10 (v / v)% human serum substitute can be selected from Irvine Scientific's Serum substitute supplement (Cat#: 99113, Irvine Scientific, USA).

[0081] FSH can be selected from Serono's Gonal-F (Cat#: S20030041 / L1938801B, Serono, Switzerland).

[0082] HCG can be selected from Serono's Profasi (Cat#: H20030198 / L4778801C, Serono, Switzerland).

[0083] 2. Preparation ...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

No PUM Login to view more

Abstract

The invention discloses a human immaturity ovum external mature culture method, which comprises: preparing for human external mature culture liquid, adding self-body mature follicle liquid to prepare for the culture liquid A, positioning the immaturity ovum of the first inter middle phase in the micro drop of the culture liquid A, adding separated and purified self-body ovum particle cell deposition in the culture liquid A to float into the culture liquid B with self-body ovum particle cell density 1-5*105/ml, adding the immaturity ovum of the first inter front phase in the micro drop of the culture liquid B.

Description

technical field [0001] The invention relates to the technical field of human assisted reproduction, in particular to a method for in vitro maturation and culture of human immature eggs with cumulus crown granulosa cells stripped off. Background technique [0002] In daily in vitro fertilization-embryo transfer (in vitro fertilization-embryo transfer, IVF-ET) clinical work, about 15% of eggs obtained in conventional ovarian stimulation cycles are immature eggs (immature oocytes, IMOs) [see reference 1] , these IMOs cannot be directly used in conventional intracytoplasmic sperm injection (ICSI) assisted reproductive therapy, and are mostly left aside and allowed to degenerate naturally [see references 1-3]. To develop and utilize these IMOs, which are mostly discarded in routine IVF / ICSI treatment work, namely corona-cumulus-denuded IMOs (corona-cumulus-denuded IMOs, ccdIMOs), regardless of assisted reproduction Both clinical work and basic scientific research have many pract...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
Patent Type & Authority Applications(China)
IPC IPC(8): C12N5/08C12N5/075
Inventor 朱小明黄荷凤朱依敏徐晨明钱羽力金帆
Owner ZHEJIANG UNIV
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap