In vitro maturing culture method for human immature ovum

Abstract

The invention discloses a human immaturity ovum external mature culture method, which comprises: preparing for human external mature culture liquid, adding self-body mature follicle liquid to prepare for the culture liquid A, positioning the immaturity ovum of the first inter middle phase in the micro drop of the culture liquid A, adding separated and purified self-body ovum particle cell deposition in the culture liquid A to float into the culture liquid B with self-body ovum particle cell density 1-5*105 / ml, adding the immaturity ovum of the first inter front phase in the micro drop of the culture liquid B.

Description

technical field [0001] The invention relates to the technical field of human assisted reproduction, in particular to a method for in vitro maturation and culture of human immature eggs with cumulus crown granulosa cells stripped off. Background technique [0002] In daily in vitro fertilization-embryo transfer (in vitro fertilization-embryo transfer, IVF-ET) clinical work, about 15% of eggs obtained in conventional ovarian stimulation cycles are immature eggs (immature oocytes, IMOs) [see reference 1] , these IMOs cannot be directly used in conventional intracytoplasmic sperm injection (ICSI) assisted reproductive therapy, and are mostly left aside and allowed to degenerate naturally [see references 1-3]. To develop and utilize these IMOs, which are mostly discarded in routine IVF / ICSI treatment work, namely corona-cumulus-denuded IMOs (corona-cumulus-denuded IMOs, ccdIMOs), regardless of assisted reproduction Both clinical work and basic scientific research have many pract...

AI Technical Summary

Problems solved by technology

[0009] 1), the successful (maturity) rate of pure IVM culture and the subsequent developmental ability of corresponding mature eggs are not as good as the corresponding indicators of cocIMOs [see reference 6, 7]

[0010] 2) MFF and feeder cells used for coIVM are mostly of allogeneic or heterogeneous origin, and there is a corresponding risk of disease transmission and / or cross-infection between allogeneic or heterogeneous [see reference 1]

[0011] 3) There are only two special research reports on autologous ovarian granulosa cells assisting human ccdIMOs CoIVM, neither of which showed advantages in IVM promotion [see reference 2, 20]

[0012] 4), no one autoMFF participated in ccdIMOs IVM special technology or research report

[0013] 5) There is no special technology or research report on the synergy between autoCCs and autoMFF for ccdIMOs coIVM

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