Polyarginine-coated magnetic nanovector and methods of use thereof
a magnetic nanovector and polyarginine coating technology, applied in the direction of tissue culture, dna/rna fragmentation, microcapsules, etc., can solve the problems of preventing any therapeutic function, affecting the passive diffusion of sirna molecules across cell membranes, and high cytotoxicity,
Inactive Publication Date: 2013-11-14
UNIV OF WASHINGTON CENT FOR COMMERICIALIZATION
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Benefits of technology
The present invention provides a nanoparticle with a polyarginine coating that can be used for magnetic resonance imaging, drug delivery, targeting, and gene silencing. The nanoparticle can also include a diagnostic agent for magnetic resonance imaging. The nanoparticle has a core with magnetic activity, and can also carry therapeutic agents and targeting agents. The nanoparticle can be used for treating tissues and detecting cells or tissues by magnetic resonance imaging.
Problems solved by technology
However, the physicochemical characteristics of siRNA (e.g., high molecular weight, anionic charge, and hydrophilic character) hinder its passive diffusion across cell membranes precluding any therapeutic function.
Furthermore, siRNA molecules are highly vulnerable to degradation.
However, the high cationic charge density of these synthetic polymers also renders them highly cytotoxic.
Method used
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[0035]The present invention provides a nanoparticle having a polyarginine coating. In certain embodiments, the nanoparticle has a core that includes a material that imparts magnetic resonance imaging activity to the particle. The nanoparticle can further include one or more of a therapeutic agent that can be delivered by the particle, a targeting agent to target the nanoparticle to a site of interest, and a diagnostic agent that allows for imaging of the particle. The therapeutic, targeting, and diagnostic agents can be covalently coupled to the nanoparticle. Methods for making and using the nanoparticles are also provided.
[0036]Nanoparticle Having Polyarginine Coating
[0037]In one aspect, the invention provides a multifunctional nanoparticle having a polyarginine (pArg) coating. In one embodiment, the nanoparticle comprises (a) a core having a surface and comprising a core material and (b) a polyarginine coating covalently coupled to the surface of the core.
[0038]As used herein, the...
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Abstract
Polyarginine-coated nanoparticle, and methods for making and using the nanoparticle. The nanoparticle can have a core that includes a material that imparts magnetic resonance imaging activity to the particle and, optionally, include one or more of an associated therapeutic agent, targeting agent, and diagnostic agent.
Description
CROSS-REFERENCE TO RELATED APPLICATION[0001]This application claims the benefit of U.S. Patent Application No. 61 / 646,101, filed May 11, 2012, expressly incorporated herein by reference in its entirety.STATEMENT OF GOVERNMENT LICENSE RIGHTS[0002]This invention was made with government support under Grant Nos. RO1CA134213 and RO1EB006043 awarded by the National Institutes of Health. The government has certain rights in the invention.STATEMENT REGARDING SEQUENCE LISTING[0003]The sequence listing associated with this application is provided in text format in lieu of a paper copy and is hereby incorporated by reference into the specification. The name of the text file containing the sequence listing is 41027_SEQ_FINAL.txt. The text file is 631 Bytes; was created on May 7, 2013; and is being submitted via EFS-Web with the filing of the specification.BACKGROUND OF THE INVENTION[0004]Small interfering RNAs (siRNAs) can silence gene expression in a highly specific manner for treating geneti...
Claims
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Login to View More IPC IPC(8): A61K47/34A61K9/50
CPCA61K47/34A61K9/5031A61K9/5094A61K49/186A61K49/1872C12N15/113C12N15/85C12N2310/14G01N33/54346G01N33/582
InventorZHANG, MIQINVEISEH, OMIDFANG, CHENKIEVIT, FORREST
OwnerUNIV OF WASHINGTON CENT FOR COMMERICIALIZATION