Syringe-shaped microorganism culture device

Abstract

The invnetion relates to a syringe-shaped microorganism culture device. Provided is a device for easily and quickly detecting a specific microorganism such as food-poisoning bacteria and pathogens. The device for detecting an objective microorganism in a specimen includes a sample collecting means, a vessel containing a medium for culturing the objective microorganism and a means for mixing the specimen with the medium. The medium includes a first medium for proliferating the objective microorganism and a second medium containing a component responding to the objective microorganism or its product. The first medium and the second medium are placed in the vessel to be separated from each other.

Description

technical field

[0001] The present invention relates to devices for detecting target microorganisms in a sample. In particular, the present invention relates to a device for simple and rapid detection of food poisoning pathogenic bacteria in a sample. Background technique

[0002] Food poisoning pathogenic bacteria mixed in food during the production, distribution and sales of food have repeatedly multiplied in food and caused severe food poisoning. Generally, food operators engaged in food production, distribution, and sales introduce the HACCP (Hazard Analysis and Critical Control Point) system to confirm the absence of food poisoning pathogenic bacteria before supplying food safely. However, with the development of circulation parts and other factors, the food has already reached consumers before the inspection results come out, which is one of the reasons for the frequent occurrence of food poisoning.

[0003] Similarly, for materials used in clinical trials, it is nec...

Examples

Embodiment approach 1

[0049] figure 1 A schematic diagram showing a typical device of the present invention. This device has the shape and structure of a commercially available Luer lock syringe (ルアロッツクテリンジ), and has a cylindrical main body 9 with a tapered front end, a plunger rod 1 slidably inserted into the main body 9, and a taper. The cap body 7 that is buckled (fastened) at the end of the shape. A sheet (or needle) 5 is attached to the tapered end, and the cap body 7 may be configured to cover the sheet (or needle) 5 . At the distal end of the plunger rod, there is a plunger 3 which provides liquid-tight contact with the inner wall of the above-mentioned cylindrical body 9 and thereby prevents the contents contained in the above-mentioned cylindrical body from leaking out. On the bottom of the cap body 7, a silicon rubber layer or the like may be laid to prevent the built-in contents of the above-mentioned main body from leaking from the tapered end or the end of the sheet. A stopper (or O...

Embodiment approach 2

[0054] Fig. 5 shows an overview of a detection method for detecting target microorganisms using the device of the present invention.

[0055] First, 25 g of a sample (sample) was added to 225 mL of buffered peptone water (BPW), and treated with a Stomacher (Stomacher) beating homogenizer to pulverize it. The resulting sample mixture was incubated at 35°C for about 22 hours (pre-enrichment culture). Aspirate a part of the obtained pre-enriched culture solution into a syringe with a capacity of 1.0 mL to 2.0 mL, and fill the syringe with 0.2 ml of the first medium and 0.2 ml of the second medium, wherein the RV medium is used as the second medium. 1 medium, MLCB medium was used as the 2nd medium. At this time, the aspirated pre-enrichment culture solution and the first medium and the second medium are mixed by contact.

[0056] The syringe was erected on a test tube rack, and cultured at 35° C. for about 22 hours (pre-enrichment culture and isolation culture). Presence of tar...

Embodiment 1

[0070] The device of the present invention was produced using a TERUMO syringe (for tuberculin with a capacity of 1 ml: SS-01T) and a TERUMO injection needle (25G x 1 inch) purchased from TERUMO Co., Ltd. (Tokyo).

[0071]Rappapore-Vassiliadis medium (RV medium) (manufactured by MERCK Co., Ltd.) was heated and dissolved in purified water at 1.5 times the normal concentration (62.7 g / l), and glycerol was added to make the concentration 0.5% (w / v) , mixed well and used as enrichment medium. Then, the usual amount (49 g / l) of MLCB agar medium (manufactured by Nissui Pharmaceutical Co., Ltd.) was heated and dissolved in purified water, and glycerin was added to make the concentration 0.5% (w / v), and mixed well , used as the isolation medium. Each medium was sterilized in an autoclave at 115°C for 15 minutes.

[0072] First, about 0.2 ml of the sterilized separation medium described above was aspirated by pulling the plunger rod of the syringe under aseptic conditions. Then, 0.2...

technical field

[0001] The present invention relates to devices for detecting target microorganisms in a sample. In particular, the present invention relates to a device for simple and rapid detection of food poisoning pathogenic bacteria in a sample. Background technique

[0002] Food poisoning pathogenic bacteria mixed in food during the production, distribution and sales of food have repeatedly multiplied in food and caused severe food poisoning. Generally, food operators engaged in food production, distribution, and sales introduce the HACCP (Hazard Analysis and Critical Control Point) system to confirm the absence of food poisoning pathogenic bacteria before supplying food safely. However, with the development of circulation parts and other factors, the food has already reached consumers before the inspection results come out, which is one of the reasons for the frequent occurrence of food poisoning.

[0003] Similarly, for materials used in clinical trials, it is nec...

Examples

Embodiment approach 1

[0049] figure 1 A schematic diagram showing a typical device of the present invention. This device has the shape and structure of a commercially available Luer lock syringe (ルアロッツクテリンジ), and has a cylindrical main body 9 with a tapered front end, a plunger rod 1 slidably inserted into the main body 9, and a taper. The cap body 7 that is buckled (fastened) at the end of the shape. A sheet (or needle) 5 is attached to the tapered end, and the cap body 7 may be configured to cover the sheet (or needle) 5 . At the distal end of the plunger rod, there is a plunger 3 which provides liquid-tight contact with the inner wall of the above-mentioned cylindrical body 9 and thereby prevents the contents contained in the above-mentioned cylindrical body from leaking out. On the bottom of the cap body 7, a silicon rubber layer or the like may be laid to prevent the built-in contents of the above-mentioned main body from leaking from the tapered end or the end of the sheet. A stopper (or O...

Embodiment approach 2

[0054] Fig. 5 shows an overview of a detection method for detecting target microorganisms using the device of the present invention.

[0055] First, 25 g of a sample (sample) was added to 225 mL of buffered peptone water (BPW), and treated with a Stomacher (Stomacher) beating homogenizer to pulverize it. The resulting sample mixture was incubated at 35°C for about 22 hours (pre-enrichment culture). Aspirate a part of the obtained pre-enriched culture solution into a syringe with a capacity of 1.0 mL to 2.0 mL, and fill the syringe with 0.2 ml of the first medium and 0.2 ml of the second medium, wherein the RV medium is used as the second medium. 1 medium, MLCB medium was used as the 2nd medium. At this time, the aspirated pre-enrichment culture solution and the first medium and the second medium are mixed by contact.

[0056] The syringe was erected on a test tube rack, and cultured at 35° C. for about 22 hours (pre-enrichment culture and isolation culture). Presence of tar...

Embodiment 1

[0070] The device of the present invention was produced using a TERUMO syringe (for tuberculin with a capacity of 1 ml: SS-01T) and a TERUMO injection needle (25G x 1 inch) purchased from TERUMO Co., Ltd. (Tokyo).

[0071]Rappapore-Vassiliadis medium (RV medium) (manufactured by MERCK Co., Ltd.) was heated and dissolved in purified water at 1.5 times the normal concentration (62.7 g / l), and glycerol was added to make the concentration 0.5% (w / v) , mixed well and used as enrichment medium. Then, the usual amount (49 g / l) of MLCB agar medium (manufactured by Nissui Pharmaceutical Co., Ltd.) was heated and dissolved in purified water, and glycerin was added to make the concentration 0.5% (w / v), and mixed well , used as the isolation medium. Each medium was sterilized in an autoclave at 115°C for 15 minutes.

[0072] First, about 0.2 ml of the sterilized separation medium described above was aspirated by pulling the plunger rod of the syringe under aseptic conditions. Then, 0.2...

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