Method of extracting succinic acid from microorganism fermentation liquor

Abstract

The invention discloses a method of extracting succinic acid from microorganism fermentation liquor. The method includes the following steps: (1) sodium succinate fermentation liquor obtained through microorganism fermentation is clarified through an ultrafiltration membrane or micro-filtration membrane for removing thalli or other large-particle impurities; (2) the clarified fermentation liquor is acidized through concentrated hydrochloric acid to reach pH ranging from 1 to 3, and the pH is preferentially 2.0; (3) the acidized liquor is subjected to reduced pressure distillation concentration to be with succinic acid concentration at 300-400g / L, and sodium chloride precipitations are removed through solid-liquid separation; (4) the filter liquor obtained in the step (3) is subjected to cooling crystallization under stirring at temperature to be 5 DEG C to obtain succinic acid solids; (5) the filter liquor obtained in the step (4) continues to be concentrated to obtain second sodium chloride precipitations; (6) the residual mother liquor is subjected to cyclic operation according to the step (4) and the step (5) to obtain succinic acid solids and sodium chloride solids; and (7) succinic acid solids and sodium chloride solids are dissolved, discolored and recrystallized to obtain high-purity succinic acid crystals and sodium chloride crystals.

Description

technical field

[0001] The invention relates to a method for extracting succinic acid from microbial fermentation broth. Background technique

[0002] Succinic acid, also known as succinic acid, is an important C4 platform compound, one of the 12 most important platform compounds announced by the U.S. Department of Energy. At present, there are four main markets for succinic acid: first, as surface active agent, cleaning agent, additive and foaming agent; second, as an ion chelating agent, used to prevent metal corrosion and pitting in the electroplating industry; third, in the food industry as an acidifier, pH modifier, flavoring substance and Antibacterial agents; the fourth is health-related products, including medicines, antibiotics, amino acids and vitamins. These four market shares of succinic acid total more than $400 million / year.

[0003] Succinic acid is also an important chemical synthesis raw material that can replace many commodities based on benzene and petro...

Examples

Embodiment 1

[0030] Embodiment 1, extract succinic acid from microbial fermented liquid

[0031] 1) 2 L of fermented liquid containing 98 g / L sodium succinate obtained through microbial anaerobic fermentation was filtered through an ultrafiltration membrane (molecular weight cut-off of 0.02 μm) to remove bacteria and large proteins to obtain a clarified fermented liquid.

[0032] 2) Slowly add concentrated hydrochloric acid with a mass fraction of 35% to the above fermented liquid under stirring condition, and stop when the pH of the solution is 2 to obtain an acidified fermented liquid.

[0033] 3) Concentrate the acidified fermentation broth at 80° C. with a rotary evaporator until the concentration of succinic acid is 320 g / L, perform solid-liquid separation through a suction filtration tank, remove the solid sodium chloride, and collect the clarified filtrate.

[0034] 4) The filtrate obtained in step 3) was cooled and crystallized at 4° C. under agitation for 2 h, and solid-liquid sep...

Embodiment 2

[0038] Embodiment 2, extract succinic acid from microbial fermented liquid

[0039] 1) 2L of fermented liquid containing 98g / L sodium succinate obtained through microbial anaerobic fermentation was passed through an ultrafiltration membrane (molecular weight cut-off of 0.02 μm) to remove bacteria and large proteins to obtain clarified fermented liquid.

[0040] 2) Under stirring conditions, gradually add concentrated hydrochloric acid with a mass fraction of 35% to the above fermentation broth for acidification, stop when the pH of the solution is 2, and obtain an acidified fermentation broth.

[0041] 3) Concentrate the acidified fermentation broth at 90°C with a rotary evaporator until the concentration of succinic acid is 350g / L, perform solid-liquid separation through a suction filtration tank, remove the solid sodium chloride, and collect the clarified filtrate.

[0042]4) The filtrate obtained in step 3) was cooled and crystallized at 4° C. under stirring conditions for ...

technical field

[0001] The invention relates to a method for extracting succinic acid from microbial fermentation broth. Background technique

[0002] Succinic acid, also known as succinic acid, is an important C4 platform compound, one of the 12 most important platform compounds announced by the U.S. Department of Energy. At present, there are four main markets for succinic acid: first, as surface active agent, cleaning agent, additive and foaming agent; second, as an ion chelating agent, used to prevent metal corrosion and pitting in the electroplating industry; third, in the food industry as an acidifier, pH modifier, flavoring substance and Antibacterial agents; the fourth is health-related products, including medicines, antibiotics, amino acids and vitamins. These four market shares of succinic acid total more than $400 million / year.

[0003] Succinic acid is also an important chemical synthesis raw material that can replace many commodities based on benzene and petro...

Examples

Embodiment 1

[0030] Embodiment 1, extract succinic acid from microbial fermented liquid

[0031] 1) 2 L of fermented liquid containing 98 g / L sodium succinate obtained through microbial anaerobic fermentation was filtered through an ultrafiltration membrane (molecular weight cut-off of 0.02 μm) to remove bacteria and large proteins to obtain a clarified fermented liquid.

[0032] 2) Slowly add concentrated hydrochloric acid with a mass fraction of 35% to the above fermented liquid under stirring condition, and stop when the pH of the solution is 2 to obtain an acidified fermented liquid.

[0033] 3) Concentrate the acidified fermentation broth at 80° C. with a rotary evaporator until the concentration of succinic acid is 320 g / L, perform solid-liquid separation through a suction filtration tank, remove the solid sodium chloride, and collect the clarified filtrate.

[0034] 4) The filtrate obtained in step 3) was cooled and crystallized at 4° C. under agitation for 2 h, and solid-liquid sep...

Embodiment 2

[0038] Embodiment 2, extract succinic acid from microbial fermented liquid

[0039] 1) 2L of fermented liquid containing 98g / L sodium succinate obtained through microbial anaerobic fermentation was passed through an ultrafiltration membrane (molecular weight cut-off of 0.02 μm) to remove bacteria and large proteins to obtain clarified fermented liquid.

[0040] 2) Under stirring conditions, gradually add concentrated hydrochloric acid with a mass fraction of 35% to the above fermentation broth for acidification, stop when the pH of the solution is 2, and obtain an acidified fermentation broth.

[0041] 3) Concentrate the acidified fermentation broth at 90°C with a rotary evaporator until the concentration of succinic acid is 350g / L, perform solid-liquid separation through a suction filtration tank, remove the solid sodium chloride, and collect the clarified filtrate.

[0042]4) The filtrate obtained in step 3) was cooled and crystallized at 4° C. under stirring conditions for ...

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