Method for removing antibiotics resistance genes (ARGs) in sewage based on disinfecting technology

Abstract

The invention discloses a method for removing antibiotics resistance genes (ARGs) in sewage based on the disinfecting technology. The method is that the ultraviolet rays and chlorination combined process is adopted to remove the antibiotics resistance genes in sewage. The method specifically comprises the steps of acquiring sewage containing the antibiotics resistance genes into a sterilizing container; respectively irradiating water samples with ultraviolet rays for 15 seconds and 30 seconds; immediately adding NaClO after irradiating to enable free chlorine to respectively reach 5mg / L, 20mg / L and 40mg / L; reacting for 120 minutes; then adding Na2S2O3 to end the reaction; filtering the water samples through a film; cutting the microorganism enriched film; extracting DNA; performing quantitative PCR; detecting the content of ARGs. According to the method, the UV and chlorination combined technology is carried out to achieve synergistic effect, so that the target gene removal rate can be effectively increased under the general UV and chlorination disinfecting dosage; the synergistic effect increases as the chlorination dosage decreases.

Description

technical field

[0001] The invention relates to the field of water treatment for environmental protection, in particular to a method for purifying antibiotic resistance genes in sewage by disinfection technology. Background technique

[0002] Antibiotic resistance genes (Antibtiotics Resistance Genes, ARGs) are a new type of pollutants. Urban sewage treatment plants are important storage places for ARGs, and their effluent is also an important source of pollution for ARGs discharged into the water environment. Controlling the impact of ARGs in the effluent of sewage treatment plants It is of great significance to reduce the pollution of ARGs in the environment and reduce the ecological risk of ARGs.

[0003] Disinfection technology is an important process section for treating pathogenic microorganisms in urban sewage and ensuring the safe discharge and reuse of sewage. Commonly used disinfection processes include chlorine disinfection, UV, and ozone disinfection. Existing r...

Examples

Embodiment Construction

[0062] Such as figure 1 Shown is a method based on disinfection technology to remove antibiotic resistance genes in sewage. Water samples were taken from a sewage treatment plant in Nanjing, which adopted the Circulating Activated Sludge (CAST) process. Collect the CAST process supernatant (secondary effluent) for disinfection experiments. The water quality and the initial ARGs content in the water samples are shown in Table 1 and Table 2.

[0063] Table 1 Disinfection treatment water quality

[0064]

[0065] Table 2 Gene content in water samples before disinfection (copies / mL)

[0066]

[0067] The collected water samples were subjected to UV light for 15s and 30s at room temperature (20±2°C), respectively, that is, the UV dose was 62mJ / cm 2 、125mJ / cm 2 ; Add NaClO immediately after the light to make the organic chlorine FC reach 5, 20, 40mg / L respectively, stir with a stirrer, add NaClO after 120min of reaction 2 S 2 o 3 (1.5%) to terminate the reaction, get 2...

technical field

[0001] The invention relates to the field of water treatment for environmental protection, in particular to a method for purifying antibiotic resistance genes in sewage by disinfection technology. Background technique

[0002] Antibiotic resistance genes (Antibtiotics Resistance Genes, ARGs) are a new type of pollutants. Urban sewage treatment plants are important storage places for ARGs, and their effluent is also an important source of pollution for ARGs discharged into the water environment. Controlling the impact of ARGs in the effluent of sewage treatment plants It is of great significance to reduce the pollution of ARGs in the environment and reduce the ecological risk of ARGs.

[0003] Disinfection technology is an important process section for treating pathogenic microorganisms in urban sewage and ensuring the safe discharge and reuse of sewage. Commonly used disinfection processes include chlorine disinfection, UV, and ozone disinfection. Existing r...

Examples

Embodiment Construction

[0062] Such as figure 1 Shown is a method based on disinfection technology to remove antibiotic resistance genes in sewage. Water samples were taken from a sewage treatment plant in Nanjing, which adopted the Circulating Activated Sludge (CAST) process. Collect the CAST process supernatant (secondary effluent) for disinfection experiments. The water quality and the initial ARGs content in the water samples are shown in Table 1 and Table 2.

[0063] Table 1 Disinfection treatment water quality

[0064]

[0065] Table 2 Gene content in water samples before disinfection (copies / mL)

[0066]

[0067] The collected water samples were subjected to UV light for 15s and 30s at room temperature (20±2°C), respectively, that is, the UV dose was 62mJ / cm 2 、125mJ / cm 2 ; Add NaClO immediately after the light to make the organic chlorine FC reach 5, 20, 40mg / L respectively, stir with a stirrer, add NaClO after 120min of reaction 2 S 2 o 3 (1.5%) to terminate the reaction, get 2...

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