Synthesis method and application of pH-responsive AIE fluorescent nanopolymer quantum dots

A fluorescent nano-synthetic method, applied in the field of fluorescent imaging, can solve problems such as limiting the practical application of organic fluorescent molecules, high toxicity, and affecting biocompatibility

CN108659154BActive Publication Date: 2020-10-30NORTHWEST NORMAL UNIVERSITY
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Patents(China)
Current Assignee / Owner
Publication Date
2020-10-30

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Abstract

The invention discloses a synthesizing method and application of pH-responsive AIE (aggregation-induced emission) fluorescence nanopolymer quantum dots. The synthesizing method includes 1), synthesizing of 4-hydroxytetrastyrene; 2), synthesizing of TPE-OH; 3), synthesizing of TPE-BMP; 4), synthesizing of TPE-PAA polymer quantum dots, namely solving crylic acid in a first solvent with stirring, sequentially adding three(2-dimethylamino-ethyl)amine, CuBr and TPE-BMP under argon protection, precipitating the product with ethanol after stirring for reaction at room temperature to obtain white flocculent precipitation which is TPE-PAA; subjecting the product to centrifugal separation and obtained solid to re-dispersion and centrifugation with a second solvent cyclically for many times, and drying the product with a vacuum oven at normal temperature to obtain white block TPE-PAA. The general and simple synthesis method is provided for synthesizing AIE functional materials with special performances, design and synthesis of the novel AIE multifunctional materials are facilitated, and the materials can be well applied to the fields of fluorescence sensors, optical devices, fluorescent biological probes and the like.
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Description

technical field

[0001] The invention belongs to the field of fluorescence imaging, and relates to a synthesis method and application of pH-responsive AIE fluorescent nanometer polymer quantum dots. Background technique

[0002] Fluorescence imaging technology has occupied an important position in the research fields of medicine and biology because of its excellent timeliness, completeness, non-invasiveness, and display of biological information in a natural state. Especially in the process of tumor research and related treatment, researchers can intuitively observe biological processes such as activity, growth, survival rate and drug absorption of experimental tumor cells through fluorescence imaging (cell imaging, in vivo imaging) technology , and then get relatively intuitive results and very effective biochemical information based on various experimental cells. The continuous development of nanotechnology is easy to prepare nanoscale fluorescent imaging agents, and it is...

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Embodiment Construction

[0042] Below in conjunction with accompanying drawing, the present invention is described in further detail:

[0043] The reagent that the present invention adopts is as follows:

[0044] 4-hydroxybenzophenone (98%), benzophenone (BP, 98%), titanium tetrachloride (TiCl 4 , 99%), tetrahydrofuran (THF, AR), zinc powder (Zn, AR), 6-bromo-1-hexanol (99%), N,N-diethylethylenediamine (N,N-Diethylenediamine, 98%), cuprous bromide (CuBr, 99%), ethyl acetate (ethyl acetate, AR), tris (2-dimethylaminoethyl) amine (Tris (2-dimethylaminoethy) amine, 99%), petroleum ether (petroleun, AR), potassium carbonate (K 2 CO 3 ,99.5%), 2-bromo-2-methylpropionyl bromide (BIBB, 98%), ethanol (EtOH, AR), the above reagents were all purchased from Anaiji. THF was purified by distillation, CuBr was refluxed in acetic acid to remove impurities.

[0045] The instrument that the present invention adopts is as follows:

[0046] All infrared spectra (IR) were measured by Nicolet AVATAR 360FT-IR infrare...