Highly effective revulsion induction method for pinellia tuber excised tuber

An in vitro and pinellia technology, applied in the field of in vitro induction of pinellia tubers, can solve the problems of impact on transplanting survival rate and cumbersomeness, and achieve the value of large-scale production application, good synchronization of occurrence, and shorten the growth cycle

Inactive Publication Date: 2008-02-13
ZHEJIANG SCI-TECH UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, in the past, the conventional tissue culture and rapid propagation of Pinellia was to cultivate Pinellia seedlings through the tissues of Pinellia, which had to go through procedures such as rooting, seedling hardening, and transplanting, which were cumbersome and affected by the survival rate of transplanting. time to form tubers

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0018] Virus-free Pinellia 2mm clustered buds and non-virus-free mother plant Pinellia three-leaf tissue-cultured seedlings of the same age were subcultured on the subculture medium until 2cm rootless buds grew; 2cm virus-free seedlings were taken and transformed into Enter MS basic medium and cultivate for 8 days, and cultivate with rooting medium. In vitro tuber induction culture, the in vitro tuber induction medium is: MS basic medium: sucrose: cytokinin: paclobutrazol: activated carbon: gibberellin: propyl dihydrojasmonate: abscisic acid: agar Flour = 1000000: 30000: 0.5: 5.0: 500: 0.005: 0.2: 0.05: 4800, weighed after 40 days, and the weight of a single tuber was 0.653 grams. The pH value of the culture medium is 5.6, containing agar 4.5g / L, conventionally sterilized by moist heat for 20 minutes, gibberellin, abscisic acid, and propyl dihydrojasmonate are sterilely added to the sterilized medium after filtration sterilization, and the light intensity 1000lx, light 10h / d,...

Embodiment 2

[0020] Virus-free Pinellia 3mm clustered buds and non-virus-free mother plant Pinellia three-leaf tissue-cultured seedlings of the same age were subcultured on the subculture medium until 3cm rootless buds grew; 3cm virus-free seedlings were taken and transformed into Enter MS basic medium and cultivate for 7 days, and cultivate with rooting medium. In vitro tuber induction culture, the in vitro tuber induction medium is: MS basic medium: sucrose: cytokinin: paclobutrazol: activated carbon: gibberellin: propyl dihydrojasmonate: abscisic acid: agar Flour = 1000000: 15000: 1.0: 0.05: 2500: 0.05: 2.0: 0.5: 5000, weighed after 40 days, and the weight of a single tuber was 0.776 grams. The pH value of the culture medium is 5.8, containing 4.7g / L agar, sterilized by conventional moist heat for 21 minutes, gibberellin, abscisic acid, and propyl dihydrojasmonate are aseptically added to the sterilized medium after filtration sterilization, and the light intensity 1200lx, light 11h / d,...

Embodiment 3

[0022] The 10mm cluster buds of detoxified pinellia and the tissue cultured seedlings of the same age non-detoxified pinellia three-leaved mother plant were subcultured on the subculture medium until 4 cm rootless buds grew; the 4 cm detoxified seedlings were taken and transformed into Enter MS basic medium and cultivate for 10 days, cultivate with rooting medium, and the composition ratio of rooting medium is: MS basic medium: naphthalene acetic acid: active carbon: agar powder: sucrose=500000: 0.2: 300: 4800: 25000, and then use centrifugal In vitro tuber induction culture, the in vitro tuber induction medium is: MS basic medium: sucrose: cytokinin: paclobutrazol: activated carbon: gibberellin: propyl dihydrojasmonate: abscisic acid: agar Flour = 1000000: 20000: 1.5: 0.5: 2500: 0.5: 20.0: 5: 4500, weighed after 40 days, and the weight of a single tuber was 0.556 grams. The pH value of the culture medium is 6.0, containing agar 5.0g / L, conventional damp heat sterilization for...

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PUM

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Abstract

The invention discloses a highly effective revulsion induction method for pinellia tuber excised tuber comprising the steps of, using successive transfer culture base for transferred culture, using root-producing culture medium for root-producing culture, using in vitro tuber induction culture medium for in vitro tuber induced culturing, wherein the successive transfer culture base comprises MS minimum medium, cytokinin, 1-naphthalene acetic acid, agar powder and cane sugar, the root-producing culture base comprises MS minimum medium, 1-naphthalene acetic acid, activated charcoal, agar powder, cane sugar, the in vitro tuber induction culture medium comprises MS minimum medium, gibberellin, abscisic acid, propyl dihydro-jasmonate, cane sugar, Paclobutrazol, cytokinin, activated charcoal and agar powder.

Description

technical field [0001] The invention relates to plant regeneration through tissue culture technology, in particular to a method for inducing isolated tubers of Pinellia ternata. Background technique [0002] Pinellia.ternata.(Thumb)Breit is a perennial herbaceous plant of Araceae. The base forms a tuber, which is the main storage organ and reproductive organ. Containing various active ingredients such as alkaloids and pinellia protein, it has many pharmacological effects such as drying dampness and resolving phlegm, reducing adverse cough, anti-early pregnancy, anti-tumor, etc. It is a unique traditional Chinese medicinal material in my country and has a large demand. Due to over-collection, the wild resources of pinellia are almost exhausted. Pinellia has three reproductive methods: seed propagation, small tuber propagation and bulbil reproduction. In natural evolution, asexual reproduction has been formed. Therefore, small tubers are generally used as reproductive organs ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01H4/00
Inventor 陈集双王海丽竺锡武何煜波张凯陈新爱黄幸雷
Owner ZHEJIANG SCI-TECH UNIV
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