The invention discloses a highly effective revulsion induction method for pinellia tuber excised tuber comprising the steps of, using successive transfer culture base for transferred culture, using root-producing culture medium for root-producing culture, using in vitro tuber induction culture medium for in vitro tuber induced culturing, wherein the successive transfer culture base comprises MS minimum medium, cytokinin, 1-naphthalene acetic acid, agar powder and cane sugar, the root-producing culture base comprises MS minimum medium, 1-naphthalene acetic acid, activated charcoal, agar powder, cane sugar, the in vitro tuber induction culture medium comprises MS minimum medium, gibberellin, abscisic acid, propyl dihydro-jasmonate, cane sugar, Paclobutrazol, cytokinin, activated charcoal and agar powder.