Nucleic acid film tape and kit for diagnosing alpha mediterranean anemia

Abstract

The invention relates to a diagnostic reagent for thalassemia, in particular to a nucleic acid membrane strip and a kit for diagnosing α-thalassemia. Nucleic acid membrane strips for diagnosing α-thalassemia, specific oligonucleotide probes for detecting α-globin gene mutation / deletion immobilized on the substrate; kits for diagnosing α-thalassemia, including: specific amplification Primers for the α-globin gene or transcript, and specific oligonucleotide probes for detecting mutations / deletions of the α-globin gene. The nucleic acid hybridization membrane strip and kit for diagnosing α-thalassemia described in the present invention have low production cost, fast detection speed and stable detection results, and can detect α-globin gene mutation while detecting α-globin gene deletion . In prenatal diagnosis, the possibility of misdiagnosing fetuses with point mutations of thalassemia major— / αTα as thalassemia minor— / αα is avoided, thereby avoiding the birth of children with thalassemia major and reducing the huge family burden and society cost.

Description

technical field [0001] The invention relates to a diagnostic reagent for thalassemia, in particular to a nucleic acid membrane strip and a kit for diagnosing α-thalassemia. Background technique [0002] Thalassemia (thalassemia, hereinafter referred to as thalassemia) is due to the decrease in the synthesis rate of one or some globin chains in the patient, resulting in the lack of some peptide chains and the relative excess of other peptide chains, resulting in an imbalance in the number of peptide chains, resulting in hemolysis sexual anemia. There are two main types of thalassemia, α-thalassemia caused by a deficiency of α-globin chain synthesis and β-thalassemia caused by a deficiency of β-globin synthesis. [0003] Alpha-thalassemia is one of the most common monogenic genetic diseases in the world. The disease occurs in a wide range from Italy, Greece, Malta, Cyprus along the Mediterranean Sea to Southeast Asian countries. Guangxi, Guangdong, Hainan, Hong Kong, Taiwan...

AI Technical Summary

Problems solved by technology

The disadvantage of this method is that it can only detect the deletion of the α gene, but not the mutation of the α gene, which can easily lead to missed diagnosis and result in the birth of defective babies

The technical solution is to use PCR technology to amplify the α-globin gene, and analyze whether the α-globin gene is missing or mutated by electrophoresis. This method is likely to cause EB pollution

[0014] So far, there are no published patents or literature reports for α-thalassemia-based nucleic acid hybridization membrane strips based on nylon membranes and mutated genes located in the middle of oligonucleotide probes

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