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Staphylococcus pasteuri LF-2 and application thereof

A Staphylococcus pasteurian, LF-2 technology, applied in the direction of bacteria, microbial-based methods, medical raw materials derived from bacteria, etc., can solve the problem of TMPD toxicity reduction

Inactive Publication Date: 2009-11-18
NORTHWEST A & F UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Currently, there is no effective way to reduce the toxicity of TMPD in Oxytropis glacialis

Method used

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  • Staphylococcus pasteuri LF-2 and application thereof
  • Staphylococcus pasteuri LF-2 and application thereof
  • Staphylococcus pasteuri LF-2 and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0038] Embodiment 1: Isolation and screening method of Klebsiella oxytocia LF-1

[0039] 1. Soil samples: collected from the environmental soil where locoweeds grow in Tibet, Inner Mongolia, Qinghai and other places;

[0040] 2. Isolation and screening of bacterial strains: Take 20g~30g of soil from 4 sources in the growth environment of locoweed and inoculate them in ordinary broth, expand the bacteria at 37°C for 48 hours, then inoculate them on ordinary agar by streaking, culture at 37°C for 24 hours, Pick a single colony and inoculate it in common broth containing 50mg / L TMPD, incubate at 37°C for 48 hours, and save the strain; inoculate the strain saved in the previous step in common broth containing 100mg / L TMPD, place at 37°C Cultivate for 48 hours; repeat the above process, increase the content of TMPD each time, reduce the content of organic nutrients in the culture solution, and preserve the strains. After 5 times of cultivation, when the content of TMPD in the medi...

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Abstract

A Staphylococcus pasteuri LF-2 is stored in CCTCC and the preservative number is M206127. It could degrade vegetable toxin and TMPD toxicity effectively and be used for grassland poisonous weeds biological control and ecological research.

Description

technical field [0001] The invention relates to a new bacterial strain of Staphylococcus pasteuri and the application of the bacterial strain in the preparation of medicines for degrading plant toxins. Background technique [0002] Locoweed is the general term for the poisonous plants of the genus Astragalus and Oxytropis in the leguminous family, and it is also the most serious poisonous weed that endangers grassland animal husbandry in the world. In my country, locoweeds are widely distributed in the vast grasslands of Northwest, North and Southwest China, with an area of ​​damage reaching 11 million ha 2 . In recent years, important progress has been made in domestic and foreign research on the hazards of locoweeds, toxic components, toxicological mechanisms, prevention and utilization, but so far there is no effective method to control the spread of locoweeds, which has caused the problem of locoweed poisoning to become more and more serious. It has become a serious pr...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20A61K35/74C12R1/44
Inventor 王建华李勤凡王岚峰耿果霞
Owner NORTHWEST A & F UNIV
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