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Method for extracting and separating bean active polypeptide and its amino-acid sequence

A technology of active peptides and leguminous plants, applied in the field of biochemical technology and biopesticides, can solve the problems of few botanical pesticides, ineffective mechanism of action, and no separation of active substances

Inactive Publication Date: 2007-09-05
SOUTH CHINA UNIV OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

But so far, although there has been a lot of research on the application of botanical insecticides in the control of stored grain pests at home and abroad, they mainly stay on the direct application of crude extracts and the compounding of crude extracts and chemical pesticides. , the specific active substance has not been isolated, the research on its mechanism of action is still very in-depth, and there are few botanical insecticide products developed.

Method used

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  • Method for extracting and separating bean active polypeptide and its amino-acid sequence
  • Method for extracting and separating bean active polypeptide and its amino-acid sequence
  • Method for extracting and separating bean active polypeptide and its amino-acid sequence

Examples

Experimental program
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Embodiment 1

[0024] (1) Preparation of crude extract rich in pea albumin: 10 kg of pea flour was mixed with 140 liters of acetic acid buffer solution of pH 4.9 while stirring, and the stirring was continued for 1 hour. The mixture was centrifuged at a speed of 7500 rpm. Under the condition that the temperature is lower than 25°C, the supernatant is subjected to M5 membrane ultrafiltration; the collected filtrate is repeated with the same membrane for ultrafiltration once, and the obtained filtrate is centrifuged at 6000 rpm for 20 minutes, and the supernatant is collected and frozen dry. The lyophilized powder of the crude extract, which is about 1% of the amount of material, is obtained, which is used for the separation of different components and the toxicity test of each component.

[0025] (2) Anion exchange chromatography separation: take 10 g of the freeze-dried powder of the crude extract obtained in step (1), mix it with 100 ml of 60% methanol, and stir at 4° C. for 1 hour. Then ...

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Abstract

A process for extracting and separating the active polypeptide from pea includes such steps as preparing coarse pea protein, separating by chromatography with ion exchange resin, and purifying by reverse-phase HPLC chromatography. Its amino acid sequence is also disclosed.

Description

technical field [0001] The invention belongs to the technical field of biochemical technology and biopesticides, and particularly relates to a method for separating and extracting active polypeptides of leguminous plants with anti-insect function and its amino acid sequence. Background technique [0002] Years of research have shown that the survival and reproduction of stored grain pests such as rice weevils (Sitophilus oryzae), corn weevils (Sitophilus zeamais) and grain weevils (Sitophilus granarius) are significantly reduced when grains are stored together with peas (Pisum sativum) ; In the feeding experiments of rice weevil, corn weevil, grain weevil and aphids, the protein components crudely extracted from pea showed significant toxic effects. As a biopesticide naturally occurring in nature, it is an important resource for the development of biopesticides whose safety is the primary feature in the future. Biochemical properties, toxicological t...

Claims

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Application Information

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IPC IPC(8): C07K1/14C07K14/415
Inventor 张毅许喜林
Owner SOUTH CHINA UNIV OF TECH
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