Herbicide preparing method and application
A technology of reaction time and soybean meal is applied in the field of protein hydrolyzate to achieve the effect of inhibiting the germination of weed seeds
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Embodiment 1
[0015] Add deionized water to the soybean meal and shake in a constant temperature oscillator at 60°C. After the temperature of the solution in the triangular flask is constant, adjust the pH of the solution to 8.0 with 20% ammonia water to become a substrate, and the mass ratio soybean meal: deionized water=1 : 9; add alkaline protease reaction in the substrate, shake constantly in the reaction process, the temperature is kept on 60 ℃, and dropwise add 20% ammonia water to maintain the pH value of reaction system at 8.0, and the reaction time is 6h, according to mass ratio bottom Substance: alkaline protease = 1:2; the reaction solution was placed in a water bath at 100°C and heated for 15 minutes to inactivate the enzyme, then centrifuged at 4000r / min for 20 minutes, the supernatant was frozen and dried into a dry powder. Refrigerate and set aside.
[0016] Soybean meal is generally in the shape of irregular fragments, the color is light yellow to light brown, the taste has ...
Embodiment 2
[0026] Similar to Example 1, the difference is that after adding deionized water to soybean meal, shake in a constant temperature oscillator at 60°C, after the temperature of the solution in the conical flask is kept constant, adjust the pH of the solution to 7.8 with 20% ammonia water to become the substrate, press Mass ratio soybean meal: deionized water = 1: 8; add alkaline protease reaction in the substrate, shake constantly during the reaction, keep the temperature at 60°C, and add 20% ammonia water dropwise to maintain the pH value of the reaction system at 7.8, The reaction time is 5 hours, according to the mass ratio of substrate:alkaline protease=1:1; the reaction solution is placed in a water bath at 100°C and heated for 10 minutes to inactivate the enzyme, then centrifuged at 3800r / min for 15 minutes, and the supernatant is frozen and dried into Dry powder, keep refrigerated for later use.
Embodiment 3
[0028] Similar to Example 1, the difference is that after adding deionized water to soybean meal, shake in a constant temperature oscillator at 60°C, after the temperature of the solution in the conical flask is constant, adjust the pH of the solution to 8.5 with 20% ammonia water to become the substrate, press Mass ratio soybean meal: deionized water=1: 10; add alkaline protease reaction in the substrate, shake constantly during the reaction, keep the temperature at 60°C, and add 20% ammonia water dropwise to maintain the pH value of the reaction system at 8.5, The reaction time is 8 hours, according to the mass ratio of substrate:alkaline protease=1:3; the reaction solution is placed in a 100°C water bath and heated for 20 minutes to inactivate the enzyme, then centrifuged at 4200r / min for 25 minutes, and the supernatant is frozen and dried into Dry powder, keep refrigerated for later use.
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