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Mulberry bark extract with glycosidase inhibiting function and preparation thereof

A Morus alba extract and a technology for inhibiting glycosidase are applied in the fields of traditional Chinese medicine extracts and preparation methods and quality control, and can solve the problems of not involving 1-deoxynojirimycin quality control indicators and the like

Active Publication Date: 2008-11-05
BEIJING WBL PEKING UNIV BIOTECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The preparation process of Cortex Morus alba extract involved in the above several patents does not involve the use of weak acid type cation resin to purify DNJ in Cortex Morus alba, and its quality control indicators are all total alkaloids, and do not involve the use of 1- Deoxynojirimycin (DNJ) is the quality control indicator, and there is no report of 1-deoxynojirimycin (DNJ) content greater than 50%

Method used

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  • Mulberry bark extract with glycosidase inhibiting function and preparation thereof
  • Mulberry bark extract with glycosidase inhibiting function and preparation thereof
  • Mulberry bark extract with glycosidase inhibiting function and preparation thereof

Examples

Experimental program
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Effect test

Embodiment 1

[0057] Embodiment 1: the preparation method of extract of the present invention

[0058] After cutting 10kg of raw medicinal material Cortex Mori, extract 3 times with water as solvent, the extraction time is 2 hours, 1 hour, and 1 hour respectively, the extraction temperature is 50°C, and the amount of water added is 70L of the weight of the raw medicinal material of Morus alba , 50L, and 50L times; the extracts were combined, concentrated under reduced pressure at 60°C and centrifuged to obtain about 40L of supernatant, which was put on a 001*7 gel-type cation exchange resin column with a volume of 5L, and the pH value of the upper column liquid 4-5, the pH of the effluent drops to 1-2, elute with deionized water until the pH value is 5-6, then elute with 25L ammonia water with a concentration of 0.5M, concentrate the ammonia water eluate under reduced pressure and evaporate Ammonia water, pH 8-9, put the concentrated solution on a 201*4 gel strong base anion exchange resin ...

Embodiment 2

[0059] Embodiment 2: the preparation method of extract of the present invention

[0060] 1 kg of the raw medicinal material Cortex Mori was cut and extracted 4 times with 15% ethanol solution as a solvent. The extraction time was 3 hours, 2 hours, 1 hour, and 1 hour respectively. The extraction temperature was 80°C, and the amount of solvent was respectively 8L, 6L, 5L, 5L of the weight of the white bark raw material; the extracts were combined, concentrated under reduced pressure at 55°C and centrifuged to obtain 3L supernatant, which was put on a 001*7 gel-type cation exchange resin column with a column volume of 0.4 L, the pH value of the upper column liquid is 4-5, and the pH of the effluent liquid is reduced to 1-2, eluted with deionized water until the pH is 5-6, and then eluted with 1.2L ammonia water with a concentration of 1.0M, and the ammonia water The eluate is concentrated under reduced pressure and evaporated to exhaust the ammonia water. The pH value is 9. The c...

Embodiment 3

[0061] Embodiment 3: the preparation method of extract of the present invention

[0062] After 1 kg of the raw medicinal material Morus alba was cut, it was extracted twice with water as a solvent. The extraction time was 3 hours and 2 hours respectively, the extraction temperature was 30°C, and the amount of solvent was 10 times and 8 times the weight of the raw medicinal material Morus alba. times; the extracts were combined, concentrated under reduced pressure at 70°C and then centrifuged to obtain 3L of supernatant, passed through an activated carbon column to remove most of the pigment, and eluted with 8L of 15% ethanol, and 001 on the 15% ethanol eluent *Type 4 strong acid cation exchange resin column, the column volume is 0.5L, the pH value of the upper column liquid is 6, and the pH value of the effluent liquid drops to 1~2, elute with deionized water until the pH value is 5~6, and then use 4.0L The concentration is 0.2M ammonia water for elution, the ammonia water elu...

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Abstract

The invention discloses a white mulberry root-bark exact with the glucosidase inhibition function, a preparation method thereof and a quality control method. During the preparation of the white mulberry root-bark extract, extraction, concentration and centrifugalization are adopted, and the methods of three different types of anion-cation exchange resin columns, drying and so on are carried out to fully extract and highly concentrate the effective drugs; at the same time, the invention further provides the quality control method for carrying out the content measurement of the extract.

Description

technical field [0001] The invention relates to a traditional Chinese medicine extract and its preparation method and quality control method, in particular to a traditional Chinese medicine extract with glycosidase inhibitory effect and its preparation method and quality control method. Background technique [0002] Morus alba is the dried root bark of Moraceae mulberry with cork removed. Usually, the roots are excavated before the leaves fall in late autumn and germinate in spring, the yellow cork is scraped off, the root bark is stripped, and dried in the sun to be used as medicine. The 1-deoxynojirimycin (DNJ) and its derivatives and other alkaloids contained in Morus alba bark are α-glucosidase inhibitors with clear effects. [0003] In the patent document whose application number is 01113191.8, the patent name is "Chinese medicine extract with α-glucosidase inhibitory activity, its preparation method and application" discloses a method for preparing total alkaloids of C...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K36/605A61P3/10G01N30/02A61K125/00
Inventor 段震文郭树仁樊利青
Owner BEIJING WBL PEKING UNIV BIOTECH
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