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Chlamydia trachomatis specific oligonucleotide sequences

A kind of Chlamydia trachomatis and oligonucleotide technology, which is applied in the reagent of nucleic acid amplification test, in the field of detection of Chlamydia trachomatis, and can solve problems such as obstacles and low-sensitivity tests.

Inactive Publication Date: 2008-11-12
SIEMENS HEALTHCARE DIAGNOSTICS INC
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0009] [8] However, existing nucleic acid amplification assays for Chlamydia detection still exhibit certain shortcomings and limitations
Although these assays have been designed to minimize contamination, there are still barriers to replacing less sensitive tests with this relatively new technology

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0150] Example 1: Specificity of Chlamydia trachomatis / Neisseria gonorrhea multiplex analysis

[0151] [108] Use multiplexed TaqMan kPCR analysis to test fifteen (15) different Chlamydia trachomatis (CT) serotypes (ie, A, B, Ba, C, D, E, F, G, H, I, J , K, L1, L2 and L3) and forty-six (46) different Neisseria gonorrhea (GC) isolates.

[0152] [109] Using Stratagene’s Mx3000P TM The real-time PCR system (Stratagene Inc., San Diego, CA) performs amplification and detection in separate, sealed reaction wells. The analytical master mix used in these experiments contains Taq DNA polymerase, buffer, reference dye (ROX) and MgCl 2 , AmpErase TM UNG (1 unit / μL), from Applied Biosystems (Perkin-Elmer Applied Biosystems, Foster City, CA) or QIAGEN (Hilden, Germany); Oligonucleotide primers and probes were synthesized by themselves or purchased from BioSearch Inc. The kPCR reaction mixture consists of 25 μL of master mix and 25 μL of purified DNA.

[0153] [110] The results obtained are rep...

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PUM

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Abstract

The present invention relates to oligonucleotide sequences for amplification primers and detection probes and to their use in nucleic acid amplification methods for the selective and specific detection of Chlamydia trachomatis in biological samples. The invention also provides oligonucleotide primer sets and primer / probe sets in the form of kits for the detection and diagnosis of chlamydial infection. The inventive oligonucleotide primers and probes can also be used in combination with other specific oligonucleotide primers and probes for the simultaneous detection ofChlamydia trachomatis and other target organisms, such as Neisseria gonorrhea.

Description

[0001] Related application [0002] [1] This application claims the priority of provisional application No. 60 / 734,155 entitled "Chlamydia trachomatis specific oligonucleotide sequence" filed on November 7, 2005. This provisional application is incorporated here by full reference. Background of the invention [0003] [2] Chlamydia is a widespread intracellular bacterial pathogen responsible for a variety of important human and animal infections. Chlamydia is an obligate, non-moving, gram-negative bacterium, characterized by a unique biphasic life cycle, with a dimorphic form that is functionally and morphologically different. Chlamydiatrachomatis, one of the four main species of the Chlamydia family, is almost a proprietary human pathogen. It is the most common cause of sexually transmitted diseases and preventable blindness in the world. There are 15 different serotypes of Chlamydia trachomatis, including serotypes A, B, Ba, and C that cause trachoma (a bilateral keratitis that af...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/68
CPCC12Q1/6818C12Q1/689C12Q2600/16C12Q2563/107
Inventor L·库C·布什-多诺文D·谢尔曼Q·蒙
Owner SIEMENS HEALTHCARE DIAGNOSTICS INC
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