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Application of yew polysaccharide in preparing medicament for myocardial ischemia-reperfusion injury

A technology of yew polysaccharides and reperfusion injury, which is applied in drug combinations, cardiovascular system diseases, pharmaceutical formulations, etc., can solve problems such as unsatisfactory results, prevent and control myocardial ischemia-reperfusion injury, and inhibit apoptosis. Effects of death, increase and protein expression

Inactive Publication Date: 2010-09-01
台州市中心医院 +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] For myocardial ischemia-reperfusion injury, drugs such as calcium ion antagonists, β-receptor antagonists, magnesium ion preparations, and oxygen free radical scavengers are commonly used to prevent and control arrhythmia induced by myocardial ischemia-reperfusion injury, The occurrence of cardiovascular diseases such as sudden cardiac death and cardiac depression caused by cardiac dysfunction; but no really satisfactory results have been obtained so far

Method used

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Examples

Experimental program
Comparison scheme
Effect test

experiment example 1

[0018] 1. The experimental animals were 30 Beagle dogs, half male and half female, body weight 8.0-10.5kg, 8-12 months old, and the license number was SCXK (Sichuan) 2004-15. The model of myocardial ischemia-reperfusion injury of Beagle dogs was established. Randomly divided into 4 groups of models of 1st, 2nd, 3rd and 4th.

[0019] 2. The drug used in the experiment was a low dose of 72 mg / KG / d (dog weight) of yew polysaccharide for one group, 290 mg / KG / d (dog weight) of yew polysaccharide for the second group, and 1 mg carvedilol for the third group / KG / d (dog weight), four groups use blank test.

[0020] 3. Experimental method

[0021] 7 days before ischemia, each experimental animal group was fed with the corresponding group of experimental drugs, twice a day, 7 days after the feeding, the ischemia was surgically ligated, and the blood was perfused after 60 minutes of ischemia. At the same time, conventional physiological instruments were used to detect the time of each ...

experiment example 3

[0032] Experimental animal is identical with experimental example 1, is divided into 3 groups of models of eight, nine, ten at random, eight groups use yew polysaccharide low dose 72mg / KG (dog weight), nine groups use yew polysaccharide high dose 290mg / KG (dog weight). In the sham operation group (without ligation) of the blank test in ten groups, the eight groups and the nine groups were fed with the corresponding experimental drugs in the eight groups and the nine groups respectively in the first 7 days of ischemia, and the drugs were fed twice a day for 7 days. After 60 minutes of ischemia, the blood was reperfused. The ten groups were fed with clear water 7 days before the ischemia, and the ischemia was not ligated after 7 days. Then, TUNEL staining, transmission electron microscopy, in situ hybridization, RX -PCT, Western Blotting, enzyme-linked immunosorbent assay and other techniques were used to detect FaS mRNA and its protein expression, Bel-2 mRNA and its protein expr...

Embodiment 1

[0038] The invention relates to a yew polysaccharide tablet, which is prepared by conventional pharmaceutical technology. The weight percentage of pure yew polysaccharide in the tablet is 65%, and the other is pharmaceutically approved auxiliary materials.

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Abstract

The invention discloses an application of polysaccharides of taxus mairei in the preparation of drugs for preventing and controlling the myocardial ischemia reperfusion injury. The drugs can be any pharmaceutically acceptable preparation, and the content of the polysaccharides of taxus mairei in the preparation by weight percent is not less than 60 percent; the polysaccharides of taxus mairei canimprove the LV-dp / dXmaX and SOD ability after the myocardial ischemia reperfusion, improve the myocardial compliance, significantly reduce the serum LDH, the serum cTnT and the MDA level after the myocardial ischemia reperfusion and reduce the myocardial injury; the polysaccharides of taxus mairei can significantly inhibit and lower the FaS mRNA and the protein expression thereof, the MCP-1 mRNA and the protein expression thereof and the NCF-47K mRNA and the protein expression thereof after the myocardial ischemia reperfusion, thereby further inhibiting the myocardial cell apoptosis after themyocardial ischemia reperfusion. Therefore, the polysaccharides of taxus mairei have the effects of preventing and controlling the myocardial ischemia reperfusion injury and developing a new clinicalapplication field of the polysaccharides of taxus mairei.

Description

technical field [0001] The invention relates to yew polysaccharides, in particular to the application of yew polysaccharides in the preparation of drugs for myocardial ischemia-reperfusion injury. Background technique [0002] The applicant has disclosed that the extract is yew polysaccharide in the invention patent with the authorized announcement number CN1206244C and the announcement date is June 15, 2005, and the title is "an extract from yew branches and leaves and its extraction method". Components, heteropolysaccharides mainly composed of D-glucose and a small amount of D-xylose, arabinose, galactose and glucuronic acid with β-1,3 glycosidic bonds, on its β-1,3 glucan main chain There is a small amount of β-1,6 glycosidic bond branched chain, the molecular weight is 160000-220000, and the purity of the extract is over 86.689%. The yew polysaccharide has anti-cancer effects such as immune regulation and direct tumor inhibition. [0003] Myocardial ischemia-reperfusion...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K31/715A61P9/10
Inventor 朱慧民朱天民朱婉萍孔繁智
Owner 台州市中心医院
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