Method for measuring non-protein nitrogen content in tobacco

Abstract

The invention discloses a measuring method of non-protein nitrogen content in tobaccos, which comprises the following steps: a) the tobaccos are weighted; b) the weighted tobaccos are put into plenty of acid solution or copper-bath so as to carry out heating until the protein is completely dissolved; c) the mixture is filtered and the acid solution or copper-bath in step b) is utilized to wash the filtrate; d) filter liquor is combined and constant volume is obtained; e) the filter liquor is taken for digestive treatment to obtain a constant volume; f) the filter liquor after volume limiting is removed, then NaOH solution is added, and a first equilibrium potential value is measured; g) NH4 solution is added, and a second equilibrium potential value is measured; and finally, the non-protein nitrogen content in tobaccos is measured by combining the first equilibrium potential value and the second equilibrium potential value. The measuring method provided by the invention is a simple, convenient and efficient method for measuring the non-protein nitrogen content in tobaccos.

Description

technical field [0001] The invention relates to a method for measuring components in tobacco, in particular to a method for measuring non-protein nitrogen content in tobacco. Background technique [0002] The content of each component in tobacco determines the quality of tobacco. For example, the ratio of tar to nicotine in mixed cigarettes is 11:1, while that of flue-cured tobacco is mostly 13-14:1. Therefore, compared with flue-cured cigarettes, mixed cigarettes have the largest The advantage is that the aroma is sufficient and the tar content is low. Tobacco contains protein, which will increase the bitterness of the smoke after burning, resulting in a spicy and irritating taste, so the protein has a bad effect on the smoking taste of cigarettes, but if the protein content is too low, the smoke will appear flat and tasteless And the aroma will also deteriorate. In addition, protein can be hydrolyzed into amino acids during the preparation process, and the content of ami...

AI Technical Summary

Problems solved by technology

In the determination process of this method, after digesting the precipitate, it is necessary to re-collect the ammonia released by strong alkali dist

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