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Intestinal bacteria culture method and culture medium thereof

A culture method and intestinal bacteria technology, applied in the field of intestinal bacteria culture method and its culture medium, can solve the problems of biochemical characteristics, metabolic characteristics and genetic characteristics that cannot be reflected

Inactive Publication Date: 2009-05-27
ZHEJIANG UNIV
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Problems solved by technology

However, these techniques have obvious shortcomings. They only provide the type and quantity of bacteria in the sample, but cannot reflect the characteristics of each bacterium, such as biochemical characteristics, metabolic characteristics, genetic characteristics, etc.

Method used

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  • Intestinal bacteria culture method and culture medium thereof
  • Intestinal bacteria culture method and culture medium thereof

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Embodiment Construction

[0014] Below in conjunction with accompanying drawing and specific embodiment the present invention is described in further detail:

[0015] This technique adopts the serial dilution method, that is, 2 to 5 g of fresh stool samples from the observed objects are collected and sent for inspection within 0.5 hours. Weigh 0.5 g of feces sample and put it into a test tube of 9 mL diluted solution A with glass beads, mix it on the mixer for at least 3 minutes, and dilute to 10 by serial dilution method. -1 ~10 -8 a dilution. According to the normal bacterial count range of different bacteria, different dilutions were selected for cultivation. According to the normal bacterial count range of different bacteria, different culture media were selected for partition culture. Divide the medium plates into different areas according to different coating concentrations, and add 50 μL of the above-diluted mixture to each area, and coat them evenly; place the aerobic plate under aerobic con...

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Abstract

The invention discloses a method for culturing intestinal bacteria. The method comprises the steps of putting fresh feces into a test tube of diluent A added with glass beads, blending the materials for more than 3 minutes on a blending device, diluting the materials to the dilution degree between 10<-> and 10<-8> according to a serial dilution method, partitioning a culture medium plate according to different spreading concentration, adding the same amount of diluted mixed solution to subareas respectively, uniformly spreading the mixed solution and putting the plate in a thermostat at 37 DEG C for incubation and culture. The method has the advantages of intuitively reflecting the species and quantity of bacteria in specimens, further researching and analyzing the biochemical characteristics, metabolic characteristics, genetic characteristics and the like of cultured bacteria.

Description

technical field [0001] The invention relates to a biological technology, in particular to a method for cultivating intestinal bacteria and a culture medium thereof. Background technique [0002] In the human intestine live approximately 10 14 There are about 400 to 500 species of bacteria, which is 10 to 20 times the total number of human cells. Bacteria in the intestinal tract play an important role in the growth and development of the human intestinal tract, immune function, digestion and absorption, and other functions. In order to better study the function of the normal intestinal flora and the relationship with the body, it is very important to isolate, identify and quantify the bacteria in the intestinal tract. So far, people's understanding of the normal intestinal flora is obtained by counting the number of colonies in the selective culture of stool samples. With the development of molecular biology and the application of molecular biology techniques in the field ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/10C12Q1/06
Inventor 李兰娟吴仲文陈云波陈春雷左健肖党生王建国陈瑜徐凯进
Owner ZHEJIANG UNIV
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