Polynary high-throughput genetic marking system and genetic analysis method for blue crabs

A genetic analysis method, the technology of Portunus trituberculatus, applied in biochemical equipment and methods, DNA/RNA fragments, microbial determination/inspection, etc., can solve problems that have not been systematically developed and actually applied, and achieve scientific guarantee and sustainability, avoiding blindness, reducing cumbersome effects

Active Publication Date: 2012-07-04
INST OF OCEANOLOGY - CHINESE ACAD OF SCI
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AI Technical Summary

Problems solved by technology

At present, the sequence information of Portunus trituberculatus (112 nucleotide sequences and 494 EST sequences published in the international shared database GenBank) has not been systematically developed and really applied to the pedigree identification and genetic relationship analysis required for genetic breeding

Method used

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  • Polynary high-throughput genetic marking system and genetic analysis method for blue crabs
  • Polynary high-throughput genetic marking system and genetic analysis method for blue crabs
  • Polynary high-throughput genetic marking system and genetic analysis method for blue crabs

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Experimental program
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Embodiment

[0048] 1) The multivariate high-throughput genetic marker system of Portunus trituberculatus is the mitochondrial sequence information system and the microsatellite molecular marker system, in which the microsatellite molecular marker is composed of system 1 with 7 SSR markers and system 2 with 6 SSR markers (see figure 1 );

[0049] System 1 consists of 7 SSR markers, and the 7 pairs of primers for amplifying the 7 SSR markers are

[0050] E13 (F: AAGGAGGAGGCTTTGATATG; R: GTCTTCCACTCCCTACTGT);

[0051] P04(F: GCCACTATCTTGCTGAGGTTGA; R: GCCATAGCACGAACACTTTTGA);

[0052] E20 (F: CCTCAGGGCAGCTCTGGAT; R: CCCGGACTAGGTGACAGGTT);

[0053] E24 (F: AACTATGGGTCCTGAGTATTA; R: CTCGGTTTCTGTCCACTT);

[0054] C13(F: CTGTCTGATGAGTGAGGCTAC; R: TGACCACGAGGAAAGGAG);

[0055] C31 (F: TATAAGGGCTTAAGTGTACG; R: GATCTAAACTCAAGGCAAAA);

[0056] H4(F: AATCACTTCACTACACCCTTTT; R: CTTGATGGGTGGCAGTCT);

[0057] System 2 consists of 6 SSR markers, and the 6 pairs of primers for amplifying the 6 SSR m...

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Abstract

The invention relates to the field of aquaculture genetics and breeding, and in particular to a polynary high-throughput genetic marking system and a genetic analysis method for blue crabs. The polynary high-throughput genetic marking system comprises a mitochondrial sequence information system and a simple sequence repeat molecular marking system, wherein the simple sequence repeat molecular marking system consists of seven SSR marking systems 1 and six SSR marking systems 2. The method comprises the following steps: using information on sequences of three-pair primer amplified COI, 16S and CR of a mitochondrion as a molecular marker; separating the offspring of the same female parent and the offspring of different female parents in a mixed breed pedigree according to genetic structures and genetic diversity; and performing male parent separation of the offspring subjected to female parent separation according to the genetic direction and the genetic diversity by using simple sequencerepeat molecular markers to complete the pedigree verification in the genetic and breeding process of the blue crabs and the confirmation of an inbreeding coefficient. On the basis of the effective use of genetic information of species, the method uses the polynary high-throughput genetic marker system to differentiate the genetic relationship between offspring and effectively avoids inbreeding depression at the same time.

Description

technical field [0001] The invention relates to the field of aquatic genetics and breeding, in particular to a multivariate high-throughput genetic marker system and a genetic analysis method of Portunus trituberculatus. Background technique [0002] With the development of biotechnology, molecular markers are constantly being updated. Currently, the markers widely used in marine animal genetics analysis are mitochondrial sequences and microsatellite markers. Mitochondrial sequence (mtDNA), as extranuclear genetic material, has the characteristics of fast evolution, non-recombination variation, and maternal inheritance. The identical sequence information indicates that the samples come from the same maternal parent. The mtDNA fragments commonly used for intraspecies genetic analysis are COI, 16S and CR. Microsatellites (SSRs) are tandem repeat sequences composed of 2-6 bases that exist in eukaryotic and prokaryotic genomes, and are highly polymorphic and co-dominant. Micro...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12Q1/68C12N15/11
Inventor 崔朝霞王鸿霞谭烽刘媛吴丹华
Owner INST OF OCEANOLOGY - CHINESE ACAD OF SCI
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