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An Improved Culture Method for Promoting the Development of Mouse Clone Embryos

A technology for cloning embryos and mice, applied in tissue culture, biochemical equipment and methods, cells modified by the introduction of foreign genetic material, etc., to achieve the effect of improving the development rate

Inactive Publication Date: 2012-02-08
赵巍
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, the long-term effects of these drugs on cloned embryos, especially whether they can be safely used in therapeutic cloning, remains to be studied.

Method used

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  • An Improved Culture Method for Promoting the Development of Mouse Clone Embryos
  • An Improved Culture Method for Promoting the Development of Mouse Clone Embryos
  • An Improved Culture Method for Promoting the Development of Mouse Clone Embryos

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Experimental program
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Embodiment Construction

[0014] Materials and Methods

[0015] 1 reagent

[0016] Unless otherwise specified, all reagents used in this study were products of Sigma (St.Louis, MO).

[0017] 2 test animals

[0018] B6D2F1 mice (C57BL / 6×DBA / 2, 8-12 weeks) were used to provide oocytes and granulosa cells, and C57BL / 6 (7-8 weeks) and ICR female mice (8-12 weeks) were used to provide fertilized eggs. 8-12 weeks ICR female mice also served as pseudopregnant recipients. The 8-12 week old ICR male mice are used as normal breeding male mice, and secondly, ligated and used as pseudopregnant male mice. DBA male mice, C57BL / 6 and ICR female mice and ICR male mice were purchased from Beijing Weitong Lihua Co., Ltd. B6D2F1 mice were bred in this laboratory, and all mice were kept in a clean environment.

[0019] 3 medium

[0020] Table 1 The components of each culture medium

[0021]

[0022]

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Abstract

The present invention relates to a method for improving the developmental rate of mouse cloned embryos. The method is a continuous culture method-D culture method. The present invention also relates to two culture systems for improving the developmental rate of mouse cloned embryos. The present invention also relates to the use of A method for obtaining cloned animals using the culture system, and a method for obtaining embryonic stem cells using the culture system. The invention improves the developmental rate of mouse cloned embryos and the establishment efficiency of nuclear transplanted embryonic stem cells, opens up new ideas for the application research of cloning technology in production, and contributes to the further development of animal cloning.

Description

technical field [0001] The present invention relates to a method for improving the developmental rate of mouse cloned embryos. The method is a continuous culture method-D culture method. The present invention also relates to two culture systems for improving the developmental rate of mouse cloned embryos. Using the culture system A method for obtaining somatic cell cloned animals, and a method for obtaining embryonic stem cells using the culture system. Background technique [0002] In recent years, the technique of somatic cell nuclear transfer (SCNT) has achieved success in many species, but the efficiency of SCNT is still relatively low. Studies suggest that the inefficiency of somatic cell nuclear transfer (SCNT) is largely caused by incomplete donor nuclear reprogramming (Jouneau et al., 2003; Latham 2004). In order to improve the efficiency of reprogramming, many methods have been tried, including the activation timing of reconstituted embryos and the influence of DM...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N5/16C12N5/18A01K67/027
Inventor 周琪代相鹏郝捷王柳
Owner 赵巍
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