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Polypeptide composition for detecting immune antibody of rheumatoid arthritis in vitro

A peptide composition, in vitro detection technology, applied in the direction of peptides, hybrid peptides, specific peptides, etc., can solve the problem of waiting for further independent clinical research, the detection sensitivity cannot be significantly improved, and there is no data to support BiP rheumatoid arthritis. And other issues

Active Publication Date: 2013-06-19
SHANGHAI RONGSHENG BIOLOGICAL PHARM CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

64% of patients with rheumatoid arthritis can produce antibodies directly against BiP, and there are reports that it is highly specific to the disease, but there is no data to support the role of BiP in predicting rheumatoid arthritis, and these reports The BiP antibody still needs to be confirmed by independent clinical research (Clinica Chimica Acta 2004, 350, 17-34)
Although these cyclic or linear polypeptides derived from filaggrin or artificially designed have high specificity (>90%) in the detection of rheumatoid arthritis antibodies, but due to the presence of numerous HLA -DR alleles, the resulting antibodies are also correspondingly different, which makes the detection sensitivity not significantly improved (<70%)

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0143] The synthesis of embodiment 1 polypeptide I

[0144] Polypeptides are synthesized by solid-phase synthesis technology using Fmoc chemical method. For the specific steps of this method, see Eur.J.Immunol.1994, 24, 3188-3193; J.Org.Chem.1972, 37, 3404-3409; Huang Weide, Chen Changqing Polypeptide Synthesis, Beijing: Science Press, 1985.

[0145] The formation method and steps of disulfide bonds can be found in the literature: Huang Weide, Chen Changqing Peptide Synthesis, Beijing: Science Press, 1985, p85; Michael W.Pennington Peptide Synthesis Protocols (Methods in Molecular Biology), Humana Press, 1994, p91-169 .

[0146] Through the above steps, the specific sequence of the synthetic polypeptide is:

[0147] Polypeptide I: His-Gln-Cys-Ala-Arg-Phe-Gln-Met-Arg-His-Cit-Arg-Leu-Ile-Arg-Cys-Gly, on which the third and sixteenth positions of Cys pass through the sulfhydryl group Form a disulfide bond, and make the polypeptide form a ring structure, and can simulate the β-...

Embodiment 2

[0148] The synthesis of embodiment 2 polypeptide II

[0149] Polypeptide II was synthesized according to the method disclosed in Example 1: His-Gln-Cys-Ala-Arg-Phe-Gln-Met-Cit-His-Cit-Arg-Leu-Ile-Arg-Cys-Gly, on which the third The Cys at the sixteenth position and the sixteenth position form a disulfide bond through the sulfhydryl group, and make the polypeptide form a ring structure, and can simulate the β-turn structure.

Embodiment 3

[0150] The synthesis of embodiment 3 polypeptide III

[0151] Polypeptide III was synthesized according to the method disclosed in Example 1: His-Gln-Cys-His-Gln-Phe-Arg-Phe-Cit-Gly-Cit-Ser-Arg-Ala-Ala-Cys-Gly, on which the third The Cys at the sixteenth position and the sixteenth position form a disulfide bond through the sulfhydryl group, and make the polypeptide form a ring structure, and can simulate the β-turn structure.

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PUM

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Abstract

The invention discloses a polypeptide composition for detecting an immune antibody of rheumatoid arthritis in vitro. At least one arginine side chain in various polypeptide sequences is modified to be electro-neutral or electro-negative amino acid. Cyclic polypeptide is produced by forming a disulfide bond with two non-adjacent cysteine side chain sulfhydryl groups in the sequence. The polypeptide not only can be combined with the autoimmune antibody of the rheumatoid arthritis, but also have high affinity to human leukocyte antigen DR (HLA-DR). Experiments prove that the specificity for the combination of the polypeptide and the autoimmune antibody of the rheumatoid arthritis is more than 90 percent and the flexibility for the detection of the autoimmune antibody of the rheumatoid arthritis is more than 75 percent. Compared with the similar products sold on the market, the polypeptide composition has obviously improved sensitivity so as to more contribute to detecting the immune antibody of the rheumatoid arthritis in vitro.

Description

technical field [0001] The present invention relates to a polypeptide composition combined with an immune antibody, in particular to a polypeptide composition combined with a rheumatoid arthritis immune antibody, and more particularly relates to a polypeptide having at least one arginine modified for use in Polypeptide composition for in vitro detection of rheumatoid arthritis autoimmune antibodies. Background technique [0002] Clinically, autoimmune diseases mainly include systemic lupus erythematosus, rheumatoid arthritis, Sjogren's syndrome, dermatomyositis, polymyositis, systemic (multiple) sclerosis, scleroderma, etc. These diseases were once named " Connective tissue disease", later both abroad and domestically classified them as rheumatic diseases. [0003] Rheumatoid arthritis (RA) is a relatively common systemic autoimmune disease mainly manifested by chronic polyarticular inflammation. Very painful. The incidence of the disease is 0.5-1% worldwide, and the inci...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N33/53C07K7/08C07K19/00
Inventor 朱绍荣
Owner SHANGHAI RONGSHENG BIOLOGICAL PHARM CO LTD
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