Anhydrous transportation method for medaka zygotes

A transportation method and technology of fertilized eggs, which is applied in the field of rapid transportation of medaka fertilized eggs, can solve the problems of broken transportation bags, waste of manpower and material resources, etc., and achieve the effects of convenient transportation, fast success rate, and easy communication

Inactive Publication Date: 2010-09-15
EAST CHINA SEA FISHERIES RES INST CHINESE ACAD OF FISHERY SCI
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AI Technical Summary

Problems solved by technology

[0004] Existing medaka transportation generally adopts the method of oxygenating with water. This method of transportation requires a special escort, which

Method used

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  • Anhydrous transportation method for medaka zygotes
  • Anhydrous transportation method for medaka zygotes

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Experimental program
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Embodiment Construction

[0009] The preparation before transportation is carried out in the following steps:

[0010] Collect medaka fertilized eggs, observe the development period of fertilized eggs with a stereo microscope, select fertilized eggs 3 within 2 hours to 6 hours of fertilization time (see attached picture) are more suitable for transportation, and pick out white and opaque unfertilized eggs at the same time;

[0011] 2-5mL plastic flat-bottomed centrifuge tube 1 with a cover (see attached picture) is used as the egg storage device, which has a good moisturizing effect and is convenient for transportation;

[0012] Prepare solid oxygen beads 4 for aquariums (see attached figure), and fill them into centrifuge tubes with 1 / 5 to 2 / 5 volume.

[0013] The specific operation process of anhydrous and solid oxygen oxygenation transport method is as follows:

[0014] First, select the fertilized eggs within 2 hours to 6 hours of fertilization time, remove the whitish and opaque dead eggs, add Ri...

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Abstract

The invention discloses an anhydrous transportation method for medaka zygotes, which relates to the technology of fish zygote transportation. In order to solve the technical problems, the invention provides the transportation method capable of keeping appropriate humidity and dissolved oxygen at the normal temperature so as not to affect the normal growth of the zygotes. The invention adopts the technical scheme that the method comprises the following steps of: selecting the zygotes of which fertilization time is 2 to 6 hours, removing whitish opaque dead eggs, flushing the zygotes by using Ringer's culture solution and then draining the water, filling the zygotes into a 2-5mL centrifuge tube provided with a plastic flat bottom and a cover and filled with solid oxygen beads to ensure thatthe total volume of the zygotes is about 4/5 of that of the centrifuge tube, covering the cover and then sealing the centrifuge tube by using a sealing tape, filling the sealed centrifuge tube into an A4-sized kraft envelope bag, and then putting the envelope bag into an EMS envelope to perform sealed transportation. The method is suitable for quickly transporting the medaka zygotes in an expressmode.

Description

technical field [0001] The invention belongs to fish fertilized egg transportation technology, in particular to a simple method for quickly transporting medaka fertilized eggs by means of express delivery. Background technique [0002] Medaka (Oryzias latipes) is a small fish originating in East Asia. It is easy to raise. The adult fish is about 3cm long and is one of the smallest known vertebrates. Compared with the experimental animals zebrafish and mice, the generation is shorter, 2 to 3 months. In an artificial environment, medaka can spawn every day of the year, and the spawning time can be controlled by controlling the light time. The transparent fertilized egg makes it very convenient for embryo observation and micromanipulation. Because spawning is controllable, medaka, as a representative of vertebrates, was sent into space in 1994, completing the entire development process from fertilization to individuality, realizing the real "space breeding". Japan started re...

Claims

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Application Information

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IPC IPC(8): A01N1/02
Inventor 么宗利来琦芳周凯王慧
Owner EAST CHINA SEA FISHERIES RES INST CHINESE ACAD OF FISHERY SCI
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