Methods of restoration of erectile function

A technology of dysfunction and cells, applied in the field of improving erectile dysfunction, maintaining intracavernosal pressure and improving erectile dysfunction caused by diabetes, and administering endothelial progenitor cells to the patient's body, can solve the problem of not recognizing that taking, have problems such as heart disease

Inactive Publication Date: 2010-09-29
WAKE FOREST UNIV HEALTH SCI INC
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Therefore, many men who suffer from ED may also have heart disease and are unaware of the dangers of taking oral medications

Method used

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  • Methods of restoration of erectile function
  • Methods of restoration of erectile function
  • Methods of restoration of erectile function

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0053] Example 1 Material

[0054]All chemicals were purchased from Sigma (St. Louis, MO) unless otherwise stated. ECL buffer and 125I-Sodium were purchased from PerkinElmer NEN (Boston, MA). Type II collagenase (1 mg / ml) was purchased from Boehringer Mannheim (Mannheim, Germany). Endothelial cell medium (EBM-2) was purchased from Cambrex Bio Science (Walkersville, MD). PCR reagents and primers, M199 medium, fetal bovine serum (FBS) and penicillin were purchased from Life Technologies (Gaithersburg, MD). Basic fibroblast growth factor (bFGF) was a kind gift from Judith Abraham, (Scios Nova, CA). FITC-labeled monoclonal anti-human CD31 antibody was purchased from Santa Cruz (Santa Cruz, CA). Rabbit polyclonal anti-von Willebrand factor (vWF), anti-smooth muscle actin antibody and FITC-labeled anti-rabbit IgG antibody were purchased from DAKO (Glostrup, Denmark). Monoclonal anti-human CD31, goat polyclonal anti-VE-cadherin (VE-Cad), goat anti-vWF and rabbit polyclonal ant...

Embodiment 2

[0055] Example 2 Injection of endothelial progenitor cells for improving erectile dysfunction

[0056] This study demonstrates that EPCs obtained from peripheral blood can be used to obtain mature endothelial cells. In a rat model of diabetes, endothelial cells injected into the diseased body were able to restore normal erectile function. This cell-based technology could become a viable treatment for diabetic patients suffering from erectile dysfunction.

[0057] Diabetic models were established by intraperitoneal injection of streptozotocin (50 mg / Kg). Those animals with blood glucose levels above 300 mg / dl were considered diabetic and used in this study. EPCs were isolated from the peripheral blood of donor rats. The cells are cultured, expanded and induced into an endothelial cell lineage. These cells were characterized using cell-specific markers (CD31, CD34, vWF, CD133). Culture-expanded endothelial cells were labeled with a red fluorescent dye (PKH26) and injecte...

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Abstract

A method for ameliorating erectile dysfunction (ED) in a subject, by injecting a population of cells capable of increasing nitric oxide production in corporal tissue, and maintaining the cells in vivo to increase local tissue concentrations of nitric oxide, whereby intracavernous pressure can be improved upon stimulation.

Description

Background of the invention [0001] The present invention relates to compositions and methods for treating erectile dysfunction, and more particularly to compositions and methods for administering endothelial progenitor cells (EPCs) to the body of a patient. [0002] More than 600,000 middle-aged American men experience some degree of erectile dysfunction (ED) or impotence each year, resulting in approximately 20 to 30 million American men living with ED at some point in their lives. Doctors estimate that as many as 85 percent of ED cases are caused by medical or physical problems, such as diabetes, high blood pressure, vascular disease, surgery on the prostate and urogenital area, spinal cord injury, multiple sclerosis, endocrine disorders, or medication. For example, about 35-75% of men with diabetes suffer from ED, and such patients account for about 30% of ED cases in the United States. [0003] Current methods used to treat ED include external devices, sexual therapy, sur...

Claims

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Application Information

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Patent Type & AuthorityApplications(China)
IPC IPC(8): A61P15/10A61K35/12A61M1/00A61K35/44
CPCA61K35/44A61K38/28A61P15/10A61P21/00A61P9/00A61P3/10A61K2300/00
InventorJ·柳A·阿塔拉
OwnerWAKE FOREST UNIV HEALTH SCI INC