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Transport fluid for culturing helicobacter pylori in gastric biopsy specimen and preparation method and application thereof

A technology of Helicobacter pylori and biopsy tissue, which is applied in the application, preservation of human or animal body, animal husbandry and other directions, can solve the problems of affecting the survival ability of bacteria, easy to form faults, increase viscosity, etc., and achieve good preservation effect, Easy to formulate, good water solubility

Inactive Publication Date: 2013-05-08
WENZHOU MEDICAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Both Stuart transport solution and Markus transport solution add 0.1-1% agar to increase the viscosity to isolate the air. Since the agar is solidified at room temperature, it is easy to form faults during the process of placing the specimen, so that the tissue specimen cannot be separated from the air. Completely isolated, affects the survival ability of bacteria, not a good substance to increase viscosity

Method used

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  • Transport fluid for culturing helicobacter pylori in gastric biopsy specimen and preparation method and application thereof
  • Transport fluid for culturing helicobacter pylori in gastric biopsy specimen and preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0018] The composition used for transporting liquid for Helicobacter pylori culture of gastric biopsy tissue specimen is composed of carbohydrate compound, protein compound, glycerin, sodium bicarbonate, sodium chloride and antibiotic mixed solution. Take each component by weight or volume as follows:

[0019] Maltodextrin 20g

[0020] Sucrose 80g

[0021] L-Arginine 1g

[0022] Tryptone 10g

[0023] Soy peptone 5g

[0024] Sodium chloride 50g

[0025] Sodium bicarbonate 75g

[0026] Glycerin 50ml

[0027] Calf Serum 50ml

[0028] Add distilled water to a total volume of 1000ml

[0029] The preparation process of the composition is as follows: taking sucrose, tryptone, soybean peptone and sodium chloride, dissolving them in distilled water, adding glycerin, and then sterilizing at 115° C. for 20 minutes. After cooling, first add filter-sterilized maltodextrin, L-arginine, then add sterile calf serum, then add sterile sodium bicarbonate, add 10mg, nystatin 2500IU and p...

Embodiment 2

[0032] The composition used for transporting liquid for Helicobacter pylori culture of gastric biopsy tissue specimen is composed of carbohydrate compound, protein compound, glycerin, sodium bicarbonate, sodium chloride and antibiotic mixed solution. Take each component by weight or volume as follows:

[0033] Maltodextrin 20g

[0034] Sucrose 100g

[0035] L-Arginine 5g

[0036] Tryptone 15g

[0037] Soy peptone 5g

[0038] Sodium chloride 80g

[0039] Sodium bicarbonate 100g

[0040] Glycerin 75ml

[0041] Calf Serum 70ml

[0042] Add distilled water to a total volume of 1000ml

[0043] The preparation process of the composition is as follows: weighing sucrose, peptone, tryptic soy protein and sodium chloride, dissolving them in distilled water, adding glycerin, and sterilizing at 115° C. for 20 minutes. After cooling, first add filter-sterilized maltodextrin, L arginine, then add sterile calf serum, then add sterile sodium bicarbonate, add 10mg, nystatin 2500IU and...

Embodiment 3

[0046] The composition used for transporting liquid for Helicobacter pylori culture of gastric biopsy tissue specimen is composed of carbohydrate compound, protein compound, glycerin, sodium bicarbonate, sodium chloride and antibiotic mixed solution. Take each component by weight or volume as follows:

[0047] Maltodextrin 30g

[0048] Sucrose 50g

[0049] L-Arginine 2g

[0050] Tryptone 10g

[0051] Soy peptone 10g

[0052] Sodium chloride 90g

[0053] Sodium bicarbonate 100g

[0054] Glycerin 100ml

[0055] Calf Serum 100ml

[0056] Add distilled water to a total volume of 1000ml

[0057] The preparation process of the composition is as follows: taking sucrose, peptone, tryptic soybean protein and sodium chloride, dissolving them in distilled water, adding glycerin, and sterilizing at 115° C. for 20 minutes. After cooling, first add filter-sterilized maltodextrin, L-arginine, then add sterile calf serum, then add sterile sodium bicarbonate, add 10mg, nystatin 2500IU a...

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Abstract

The invention relates to transport fluid for culturing helicobacter pylori in a gastric biopsy specimen and a preparation method and application thereof. Viscosity is enhanced, pH value is increased appropriately and anti-mixed bacterium medicaments are increased, so that the survival of the helicobacter pylori in the gastric biopsy specimen is protected and the preservation time of the specimen is prolonged. The transport fluid has high practical value. Bacteria detection rate is not affected if the specimen is submitted within 24 hours after being collected. The survival rate of the helicobacter pylori preserved at room temperature within 24 hours is over 50 percent. The transport fluid is suitable for detection in hospitals and inspection institutions in different levels.

Description

technical field [0001] The invention relates to a transport liquid for microorganism culture and its preparation method and application, in particular to a transport liquid for gastric biopsy tissue specimen Helicobacter pylori culture and its preparation method and application. Background technique [0002] Helicobacter pylori (Hp) is a pathogenic bacterium that can colonize the gastric mucosa for a long time. It is Gram-negative, curved or slightly spiral-shaped, 2.5-5mm long and 0.5mm thick. The bacteria are usually distributed in uneven groups in the gastric alveolar and glandular cavity. It can secrete a variety of exotoxins, which can damage the gastric mucosal epithelium. The secreted catalase, lipase, phosphatase, proteolytic enzymes and toxins can damage gastric epithelial cells and cause gastric mucosal inflammation. [0003] Studies have shown that Helicobacter pylori infection is closely related to gastric diseases such as chronic gastritis and peptic ulcer, and...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01N1/00
Inventor 杜季梅林刚徐昌隆刘佳明
Owner WENZHOU MEDICAL UNIV
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