Monascus mutant strain and method for preparing monascus yellow pigment by submerged fermentation thereof and article thereof
A mutant strain and deep fermentation technology, applied in the field of bioengineering, can solve the problems of difficult large-scale production, low yellow pigment content, high citrinin content, and achieve pollution-free production and safe production, high yellow pigment content. , the effect of low citrinin content
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Embodiment 1
[0046] The monascus purpureus yang TY622 mutant strain Monascus purpureus yang TY622 provided in this example has a preservation number of CGMCC4110.
[0047] The method for preparing the red yeast yellow pigment of low citrinin by submerged fermentation of the aforementioned monascus red mutant strain TY622 comprises the following steps:
[0048] 1. Strain selection:
[0049] The Monascus purpureus yang TY622 (Monascus purpureus yang TY622) isolated by ultraviolet mutagenesis was selected;
[0050] 2. Seed culture:
[0051] First use the liquid seed tank to cultivate the primary and secondary seeds, expand the cultivation and then enter the large-capacity fermenter for fermentation. The steps are:
[0052] (21) Preparation of seed liquid: adopt the high-yield Monascus red strain TY622, and cultivate it through primary and secondary seed tanks equipped with seed medium for 7 to 8 hours to obtain liquid seed culture liquid; wherein the raw materials of the seed medium are com...
Embodiment 2
[0068] The bacterial strain provided by this embodiment and preparation method, product are basically the same as Example 1, and its difference is:
[0069] 2. Equipped with culture medium
[0070] 2. Medium equipment
[0071] 2.1 Raw material composition and ratio of liquid seed medium: starch 4%, NaNO 3 0.2%, MgSO 4 0.2%, KH 2 PO 4 0.2%, corn steep liquor 3%;
[0072] 2.2 The raw material components and proportions of the fermentation medium are: rice flour 9%, NaNO 3 0.15%, MgSO 4 0.2%, KH 2 PO 4 0.25%, NH 4 Cl 0.2%, corn steep liquor 4%; use lactic acid to adjust the initial pH value of the fermentation broth to 3.0-4.0.
[0073] 3. Fermentation process
[0074] 3.1 Preparation of seed liquid: adopt a mutant strain of Monascus rubra with high yield of yellow pigment, and cultivate it in primary and secondary seed tanks for 7 to 8 hours to obtain a liquid seed culture liquid;
[0075] 3.2 Expanded cultivation: Inoculate the liquid seed culture solution in ...
Embodiment 3
[0086] The bacterial strain provided in this embodiment and preparation method, product are basically identical with embodiment 1 or 2, and its difference is:
[0087] Two, the step (3) of fermentation process " is: in 20m 3 The fermenter is filled with 13.3m 3 The above-mentioned liquid fermentation medium; inoculate in a fermenter, and the inoculum amount is 5%. The other steps are the same as in Example 1, and the pH and color value of the fermented liquid in the fermentation process are regularly detected, and the red yellow pigment product of Monascus is extracted to detect its citrinin content and color value.
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