Monascus mutant strain and method for preparing monascus yellow pigment by submerged fermentation thereof and article thereof

Abstract

The invention provides a monascus mutant strain, namely Monascus purpureus yang Ty622, and a method for preparing monascus yellow pigment by submerged fermentation of the strain. The method comprises the steps of: selection and breeding of strain, seed culture, large-capacity tank fermentation and extraction of monascus yellow pigment, wherein the seed culture and the fermentation are implemented in a specifically manufactured culture medium, and multistage fermentation is performed in a large-capacity pot fermentation tank, followed by fermentation; the technological conditions of fermentation are as below: the temperature is from 32 to 36 DEG C, the stirring rotational speed is 200 to 400r / min; the ventilation quantity at earlier stage is from 400 to 500L.min and is then regulated to 500 to 600L.min at middle / later stages. The invention further provides an article prepared according to the above method. The preparation method according to the invention has simple technology and is easy for industrialized production, and the resultant fermented liquid has high content of monascus yellow pigment and low content of citrinin.

Description

technical field

[0001] The invention relates to the technical field of bioengineering, in particular to a monascus red mutant strain and a method and a product for preparing low-citrinin monascus red yellow pigment by submerged fermentation thereof. Background technique

[0002] In the prior art, natural yellow pigments generally include: gardenia yellow, turmeric, safflower yellow, and Monascus yellow. Yellow pigment methods mainly include extraction, chemical synthesis, and microbial fermentation. Monascus is also called red yeast rice. Among the natural yellow pigments, except for Monascus yellow, the others are all extracted from plants, and its production cost is high, while Monascus yellow is produced by microbial fermentation and is easy to produce on a large scale, and its medium is corn starch with low cost, and Light and heat stability are better than other plant-derived pigments. Monascus, also known as "Danqu" and "Chiqu", has been used in Chinese food and medi...

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