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Process method for producing Wuyi biocontrol bacteria by utilizing waste residues generated by deeply fermenting Wuyiencin

A technology of submerged fermentation and wuyiencin, applied in the biological field, can solve problems such as environmental pollution, waste, and odor from waste residues, and achieve the effects of stress resistance and production increase, low production cost, and reduced emissions

Inactive Publication Date: 2011-01-19
潍坊万胜生物农药有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] In the filtration process of producing antibiotics by submerged fermentation of Wuyiencin, the plate and frame filter press is used to squeeze the clear liquid containing Wuyi antibiotics, and the remaining waste residue is usually transported to the field by farmers and used as farmland fertilizer after decay, but the waste residue In the process of fermentation and decay, peculiar smell will be produced, which will pollute the environment; in addition, the waste residue contains incompletely digested nutrient sources, mycelia of Wuyi bacteria, mature spores of Wuyi bacteria, a small amount of Wuyi bacteria exopolysaccharides, etc. Transportation of waste slag also increases production costs

Method used

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  • Process method for producing Wuyi biocontrol bacteria by utilizing waste residues generated by deeply fermenting Wuyiencin
  • Process method for producing Wuyi biocontrol bacteria by utilizing waste residues generated by deeply fermenting Wuyiencin
  • Process method for producing Wuyi biocontrol bacteria by utilizing waste residues generated by deeply fermenting Wuyiencin

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Effect test

Embodiment 1

[0019] Example 1, the process of producing Wuyi bio-control bacteria using the waste residue of Wuyiencin submerged fermentation. In the process of Wuyiensu submerged fermentation used to prepare Wuyienbacterium antibiotics, 10% of the culture medium was first put into the fermenter, and steam sterilized After miscellaneous bacteria, introduce Wuyiencin strains for submerged liquid fermentation at a temperature controlled at 35°C. After 65 hours of fermentation, a fermentation feed liquid containing Wuyienbacteria antibiotics is obtained, and then a plate and frame filter press is used to extrude the fermented material containing Wuyienbacteria The clear liquid of antibiotics is used to extract Wuyiencin. The plate and frame filter press is XX type filter press produced by Jinan Chemical Machinery Factory. Completely digested nutrient sources, mycelia of Wuyi bacteria, mature spores of Wuyi bacteria and a small amount of exopolysaccharide of Wuyi bacteria are the strain sources...

Embodiment 2

[0026] Example 2, the process of producing Wuyi bio-control bacteria using the waste residue of Wuyiencin submerged fermentation. In the process of Wuyiensu submerged fermentation used to prepare Wuyienbacterium antibiotics, 10% of the culture medium was first put into the fermenter, and steam sterilized After miscellaneous bacteria, introduce Wuyiencin strains for submerged liquid fermentation at a temperature controlled at 32°C. After 60 hours of fermentation, a fermentation feed liquid containing Wuyienbacteria antibiotics is obtained, and then a plate and frame filter press is used to extrude the fermented material containing Wuyienbacteria The clear liquid of antibiotics is used to extract Wuyiencin. The plate and frame filter press is XX type filter press produced by Jinan Chemical Machinery Factory. Completely digested nutrient sources, mycelia of Wuyi bacteria, mature spores of Wuyi bacteria and a small amount of exopolysaccharide of Wuyi bacteria are the strain sources...

Embodiment 3

[0033] Example 3, the process of producing Wuyi bio-control bacteria using the waste residue of Wuyiencin submerged fermentation. In the process of Wuyiensu submerged fermentation used to prepare Wuyienbacteria antibiotics, first put 10% of the culture medium into the fermenter, and steam After miscellaneous bacteria, introduce Wuyiencin strains for submerged liquid fermentation at a temperature controlled at 35°C. After 65 hours of fermentation, a fermentation feed liquid containing Wuyienbacteria antibiotics is obtained, and then a plate and frame filter press is used to extrude the fermented material containing Wuyienbacteria The clear liquid of antibiotics is used to extract Wuyiencin. The plate and frame filter press is XX type filter press produced by Jinan Chemical Machinery Factory. Completely digested nutrient sources, mycelia of Wuyi bacteria, mature spores of Wuyi bacteria and a small amount of exopolysaccharide of Wuyi bacteria are the strain sources for producing W...

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Abstract

The invention relates to a process method for for producing Wuyi biocontrol bacteria by utilizing waste residues generated by deeply fermenting Wuyiencin. The method comprises the following steps of: taking waste residues generated by deeply fermenting Wuyiencin, bean flour of which the weight is 8-12 relative to the weight of the waste residues, corn meal of which the weight is 18-32 relative to the weight of the waste residues, starch of which the weight is 3-8 percent relative to the weight of the waste residues and fish meal of which the weight is 3-5 percent relative to the weight of the waste residues; sterilizing and disinfecting with steam to obtain a solid fermentation medium; stirring and mixing the sterilized solid fermentation medium and the waste residues to obtain a solid culture material; and spreading the solid culture material on a culture bed by controlling the water content of the culture material to be 30-40 percent and the temperature to be 30-45 DEG C to obtain a solid culture material containing Wuyi biocontrol bacterial spores, wherein the spore content is 40-50 hundred millions / gram. The invention has low production cost, reduces the discharge of waste in the production process of the Wuyiencin, has no waste pollutants in the production process and is environmentally friendly. Proved by tests, the Wuyi biocontrol bacteria have obvious effects of disease resistance, stress resistance and production increase.

Description

technical field [0001] The invention relates to a process method for producing Wuyi biocontrol bacteria, in particular to a process method for producing Wuyi biocontrol bacteria by using Wuyiencin submerged fermentation waste residue, which belongs to the field of biotechnology. Background technique [0002] Studies have shown that plants themselves have genetic resistance to pests and diseases and immune functions and defense mechanisms. When the activities of microorganisms are used for plant protection, the antibiotics produced can directly kill and inhibit bacteria. There are also polysaccharides and polypeptides produced by beneficial microorganisms, and the signal transduction and participation in the induction of disease resistance and stress resistance of crops during the degradation process is called plant inducible systemic resistance. This kind of research has changed the plant protection model with the sole goal of sterilization, and has become an important mean...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C12R1/465
Inventor 孙波李鹏李树青王红梅雷音
Owner 潍坊万胜生物农药有限公司
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