Mink source enterococcus faecium and application thereof

A technology of Enterococcus faecium and mink, applied in the application field of fur animal diets, can solve the problems of ingestion time, supply mode and addition amount affecting the immune regulation effect, differences in immune effect, and different strains, etc.

Inactive Publication Date: 2013-01-30
SHENYANG BOYANG FEED
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AI Technical Summary

Problems solved by technology

Although probiotics have many of the above-mentioned immunomodulatory effects, for bacterial species, not only are there differences in immune effects between species, but there are also differences between strains.
In addition, the intake time, supply method and addition amount also have an impact on the immune regulation effect

Method used

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  • Mink source enterococcus faecium and application thereof

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Effect test

Embodiment

[0022] Cholesterol degradation by Enterococcus faecium MDF1104 in vitro

[0023] Inoculate the purified and cultivated Enterococcus faecium MDF1104 into the newly prepared and sterilized MRS liquid medium, culture at 37°C for 18-24 hours, take samples every four hours, centrifuge to get the supernatant, and use the o-phthalaldehyde method to determine The ability of lactic acid bacteria to degrade cholesterol within 24 hours.

[0024] Table 3 Degradation rate of Enterococcus faecium MDF1104 to cholesterol

[0025] sampling time

Cholesterol degradation rate %

4

38.45

8

32.05

12

32.05

16

32.55

24

31.07

[0026] From Table 3 and attached figure 1 It can be seen that Enterococcus faecium MDF1104 can degrade cholesterol under in vitro culture conditions, and can be degraded efficiently throughout the culture period, with an average degradation rate of 33.23%, and a maximum of 38.45% in ...

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Abstract

The invention relates to an enterococcus faecium and application thereof, in particular to an enterococcus faecium MDF1104 with the patent collection number of CGMCC (China General Microbiological Culture Collection) No.4237. The invention also relates to the application of the mink source enterococcus faecium MDF1104 on fermenting carbohydrate and degrading cholesterol, in particular to the single application or combination with the common carrier of the enterococcus faecium MDF1104 to increase mink feed intake and daily gain for the mink, enhance organism immunity performance, and prevent and cure mink seasonal diarrhea.

Description

technical field [0001] The invention relates to a strain of Enterococcus faecium isolated from mink, and particularly relates to its application in the ration of fur animals. technical background [0002] Microecological preparations are live probiotic preparations that play a beneficial role by improving the balance of the host's intestinal flora. With their advantages of green safety, no toxic side effects, and no residues, they have gradually become the main force to replace antibiotic additives. Bacteria of the genus Enterococcus are widely distributed in nature and are common in some vegetables and plants. They are also a type of microorganisms that normally exist in the digestive tract of humans and animals, and are mainly colonized in the ileum and colon. The application of Enterococcus as a probiotic mainly focuses on Enterococcus faecalis and Enterococcus faecium. Enterococcus faecium belongs to lactic acid bacteria. Because it grows fast, has good adhesion ability,...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20A23K1/16A23K10/16
Inventor 李光玉刘晗璐杨琛杰钟伟刘柏阳李丹丽王凯英孙伟丽赵靖波常忠娟赵家平
Owner SHENYANG BOYANG FEED
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