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A kind of Lactobacillus plantarum la1 for degrading cholesterol and its application

A technology of Lactobacillus plantarum and cholesterol, which is applied in Lactobacillus plantarum La1 and its application fields, can solve the problems of difficult industrialization, high fat and cholesterol, etc., and achieve the effect of increasing value and enriching varieties

Active Publication Date: 2022-03-22
河北麦克斯生物科技有限公司
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Fermented sour meat can degrade fat and cholesterol in pork under the action of microorganisms, thereby solving the problem of excessive fat and cholesterol in fatty pork, but at present, there are not many research reports in my country on the study of specific strains for preparing low-cholesterol foods , so that the preparation process of fermented sour meat is more casual and difficult to industrialize. Therefore, this application screens and isolates lactic acid bacteria capable of degrading cholesterol from traditional fermented food tempeh, obtains the dominant bacterial strain for lowering cholesterol, and then applies it to the preparation of fermented sour meat. In order to develop new functional fermented sour meat products with local characteristics, enrich the types of pork products, and increase the value of pork products

Method used

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  • A kind of Lactobacillus plantarum la1 for degrading cholesterol and its application
  • A kind of Lactobacillus plantarum la1 for degrading cholesterol and its application
  • A kind of Lactobacillus plantarum la1 for degrading cholesterol and its application

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Embodiment 1

[0020] The screening of embodiment 1 bacterial strain

[0021] Weigh 25.0g of fermented soya bean, sour cabbage, sour radish and chopped pepper, add 225.0mL of 0.9% sterilized normal saline, break up and mix well, let it stand still, absorb 0.1mL of supernatant, and carry out gradient dilution (10 -1 -10 -8 ), and spread each gradient dilution on 20mL modified MRS agar medium, make 4 parallels for each, culture at 37°C, anaerobic and aerobic constant temperature for 72h, and observe the growth of bacterial colonies.

[0022] According to the colony shape and calcium dissolution circle, pick a typical single colony, transfer it to the modified MRS medium (1mL / 1.5mL centrifuge tube), and culture it at a constant temperature of 37°C for 1d, streak into the modified MRS agar medium, and keep it at a constant temperature of 37°C After culturing for 72 hours, observe the morphological characteristics of the colonies of each strain and number them respectively.

[0023] Gram staini...

Embodiment 2

[0034] Embodiment 2 Physiological and biochemical test identification

[0035] 1.1 Growth test

[0036] Lactic acid bacteria grow well between 10°C and 45°C, and pH 9.6 is the growth limit pH of lactic acid bacteria. Therefore, by measuring the growth curves of each strain at 10°C, 45°C, and pH 9.6, we can initially identify whether the screened strains are lactic acid bacteria .

[0037] ① Growth experiment at 10°C: Inoculate the 5 isolated strains into MRS liquid medium, culture with constant temperature and shaking at 10°C, measure OD every 3 hours 560nm , Continuous measurement for 42h, draw the growth curve.

[0038] ② Growth experiment at 45°C: Inoculate the 5 isolated strains into MRS liquid medium, culture at 45°C with constant temperature and shaking, and measure OD every 3 hours 560nm , Continuous measurement for 42h, draw the growth curve.

[0039] ③ pH 9.6 growth experiment: adjust the pH of the MRS liquid medium to 9.6, inoculate 5 isolated strains, culture at...

Embodiment 3

[0069] Embodiment 3 cholesterol standard curve

[0070] Drawing of cholesterol standard curve: Accurately draw 0, 0.5, 1.0, 1.5, 2.0mL of cholesterol working solution into 10mL test tubes respectively, add glacial acetic acid to each tube to make the total volume reach 4mL. Add 1mL concentrated sulfuric acid along the tube wall, mix well, measure OD 560nm . Take the concentration of the standard solution as the abscissa, OD 560nm As the ordinate, draw the standard curve ( Figure 4 ), and establish the regression equation.

[0071] Depend on Figure 4 It can be seen that the concentration of cholesterol solution is 0mg / mL~0.25mg / mL, and OD 560nm There is a good correlation between them, the regression equation is y=2.4592x-0.0018, R 2 = 0.9958.

[0072] Sample preparation: Inoculate strains SC1, LC1, DC1, La1, and PC1 into semi-selective liquid MRS medium, culture with shaking at 37°C for 1d, absorb 0.5mL of fermentation broth, add 3.0mL of absolute ethanol and 2.0mL of...

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Abstract

The invention discloses a Lactobacillus plantarum La1 for degrading cholesterol and its application. The strain La1 has been preserved. The preservation unit: China Typical Culture Collection Center, preservation address: Wuhan University, China, preservation date: 2019 On March 4, the deposit number is CCTCC NO: M 2019121. The cholesterol degradation rate of the bacterial strain of the invention to the semi-selective MRS medium reaches 75.31%, and has practical significance for promoting the development of the sour meat industry, enriching the types of pork products, and increasing the value of the pork products.

Description

technical field [0001] The invention belongs to the technical field of food microorganism application, and in particular relates to a Lactobacillus plantarum La1 used for degrading cholesterol and an application thereof. Background technique [0002] Cholesterol is an important sterol substance in the human body. It exists widely in various tissues and cells. It plays an important physiological role in the body and is an essential nutrient for the human body. However, excessive accumulation of cholesterol in the body will also bring about various health problems. At present, cardiovascular and cerebrovascular diseases such as coronary heart disease, atherosclerosis, hyperlipidemia, and cerebral apoplexy related to high serum cholesterol levels have seriously threatened human health. Nutrition and medical experts at home and abroad recommend that the dietary cholesterol intake standard is no more than 250mg / d·person to 300mg / d·person, but with the improvement of people's livi...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20A61K35/747A61P3/06A23L33/00A23L13/70A23L5/20C12R1/25
CPCC12N1/20A61K35/747A61P3/06A23L33/00A23L13/74A23L5/28A23V2002/00A23V2400/169A23V2200/3262
Inventor 徐君飞张居作刘新路金媛悦
Owner 河北麦克斯生物科技有限公司
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