A kind of cholesterol-lowering fermented milk and preparation method thereof

A cholesterol-lowering, fermented milk technology, applied in the field of lactic acid bacteria and dairy processing

Active Publication Date: 2017-08-25
GUANGDONG YANTANG DAIRY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The present invention aims at the cholesterol-lowering effect of existing lactic acid bacteria and its deficiency in fermented milk, and aims to provide a cholesterol-lowering fermented milk and its preparation method

Method used

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  • A kind of cholesterol-lowering fermented milk and preparation method thereof
  • A kind of cholesterol-lowering fermented milk and preparation method thereof
  • A kind of cholesterol-lowering fermented milk and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0028] The effect of Lactobacillus DMDL 9010 on degrading cholesterol in vitro is very obvious, and it has good acid resistance and cholesterol degrading effect.

[0029] (1) The role of Lactobacillus DMDL 9010 in degrading cholesterol in vitro

[0030]Use o-phthalaldehyde colorimetric method (OPA method) to measure the relative content of lowering cholesterol in vitro to measure the degradation effect of cholesterol. It was placed in the MRS liquid medium with a cholesterol content of 100mg / 100mL, and the removal rate of cholesterol was 30.59±0.67%. The formula of the cholesterol-containing MRS liquid medium (MRS-CHOL medium for short) is: 100 mL of distilled water, 5.4 g of MRS medium, 0.1 g of cholesterol, 2 mL of Tween 80, and 0.3 g of ox bile salt. Dispense into centrifuge tubes. The method for preparing the MRS-CHOL medium is as follows: 1. Cholesterol is a fat-soluble substance, insoluble in water, so it is placed in Tween 80 for dissolving earlier, fully dissolved af...

Embodiment 2

[0066] In the first step, place the freeze-dried powder of DMDL 9010 in MRS (invented by de Man, Rogosa and Sharp in 1960, referred to as MRS) liquid medium, and cultivate the bacterial solution at 30°C for 16 hours to be DMDL 9010 the seed liquid;

[0067] The second step is to inoculate the MRS seed solution of DMDL 9010 obtained in step (1) into the cholesterol-containing liquid medium at a volume ratio of 10:100, and inoculate the bacterial cell solution after static cultivation at 41°C for 16 hours Inducing seed solution for DMDL 9010 with cholesterol-lowering effect;

[0068] The formula of the cholesterol-containing liquid medium is: in parts by weight, 0.8 part of peptone, 0.6 part of yeast powder, 0.5 part of beef extract, 2.0 part of glucose, 0.1 part of triamine citrate, 0.01 part of magnesium sulfate heptahydrate, 0.05 part of dipotassium hydrogen phosphate, 0.001 part of manganese sulfate tetrahydrate, 0.05 part of sodium acetate, 0.02 part of ascorbic acid, 0.05...

Embodiment 3

[0073] The first step is to place the freeze-dried powder of DMDL 9010 in MRS liquid medium, and cultivate the bacterial cell solution after 18 hours at 43°C as the seed solution of DMDL 9010;

[0074] The second step is to inoculate the MRS seed solution of DMDL 9010 obtained in step (1) into the cholesterol-containing liquid medium at a volume ratio of 5:100, and inoculate the bacterial cell solution after static cultivation at 28°C for 24 hours Inducing seed solution for DMDL 9010 with cholesterol-lowering effect;

[0075] The formula of the cholesterol-containing liquid medium is: in parts by weight, 1.2 parts of peptone, 0.2 part of yeast powder, 1.0 part of beef extract, 1.5 parts of glucose, 0.2 part of triamine citrate, 0.02 part of magnesium sulfate heptahydrate, 0.35 part of dipotassium hydrogen phosphate, 0.006 part of manganese sulfate tetrahydrate, 0.20 part of sodium acetate, 0.03 part of ascorbic acid, 0.50 part of cholesterol, 800.5 parts of Tween, 94.3 parts o...

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Abstract

The invention discloses a cholesterol-lowering fermented milk and a preparation method thereof. Fresh milk or milk powder is mainly used as a fermentation raw material, and Lactobacillus DMDL9010 is inserted into the above-mentioned raw material for fermentation production to obtain a cholesterol-lowering fermented milk; Lactobacillus sp. DMDL 9010 was deposited on August 19, 2011 in the General Microbiology Center of China Committee for Culture Collection of Microorganisms, referred to as CGMCC, and the preservation number is CGMCC NO.5172. The bacterium can degrade up to 90mg / 100mL of cholesterol in the degradation of cholesterol in vitro, and it also shows a good effect of lowering blood fat and cholesterol in rats, and has good acid resistance. The fermented milk product curd obtained in the present invention The time is right, the post-acidification is not serious, the taste is delicate, and the fermented milk with strong flavor has a simple process and convenient operation, and has important industrial value.

Description

technical field [0001] The invention belongs to the technical field of lactic acid bacteria and dairy product processing, and in particular relates to cholesterol-lowering fermented milk and a preparation method thereof. Background technique [0002] Cholesterol, as an important sterol substance in the human body, has a wide range of physiological functions in the body. However, as people’s daily dietary intake of animal foods is increasing, unreasonable dietary structure leads to an increase in the cholesterol content in the human body. It can lead to hypercholesterolemia, which can lead to cardiovascular and cerebrovascular diseases such as coronary heart disease, atherosclerosis, and stroke. At present, domestic research on cholesterol-lowering lactic acid bacteria is still in its infancy. Most scholars' research has focused on the screening of strains and their characteristics. The mechanism of action has not been explored deeply, and no effective cholesterol-lowering ba...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20A23C9/127A23L33/00C12R1/225C12R1/46
Inventor 冯立科杨爱君刘冬梅
Owner GUANGDONG YANTANG DAIRY
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