Hybrid membrane strip, PCR primer and kit for diagnosis of NSHI

Abstract

The invention discloses a hybrid membrane strip for diagnosis of NSHI (nonsyndromic hearing impairment). The membrane strip comprises a substrate and mutation detection probes, wherein each mutation detection probe respectively comprises a corresponding NSHI gene mutation locus. The gene mutation locus is at least one of the followings: a cDNA35 locus, cDNA176-191 loca, a cDNA235 locus, and cDNA299-300 loca of a GJB2 gene, a cDNA538 locus of a GJB3 gene, a cDNA1555 locus and a cDNA1494 locus of an mtDNA12srRNA gene, and a cDNA2168 locus and an IVS7-2 locus of an SLC26A4 gene; each mutation detection probe comprises sequences of 15-25 bases, and a central section of a sequence comprises a corresponding mutation base of the NSHI gene. The invention has the advantages of high accuracy and low prices, etc.

Description

technical field [0001] The present invention relates to a hybrid membrane strip used in medical diagnosis of non-syndromic deafness, and the present invention also relates to a polymerase chain reaction (Polymerase Chain Reaction, abbreviated as The term is PCR) primers; the present invention also relates to a kit for diagnosing non-syndromic deafness. Background technique [0002] Deafness is one of the most common human sensory system defects. Due to its complex etiology, high incidence and difficult treatment, it has long plagued patients and their surrounding groups, greatly affecting mutual communication and quality of life. The incidence of severe deafness in newborns is as high as 1 / 800~1 / 1000, and nearly 70 million people around the world suffer from hearing loss of more than 55 decibels. [0003] There are many reasons for deafness, and genetic factors are the main reason. Deafness can be caused by mutations in a single gene or by compound mutations in different g...

AI Technical Summary

Problems solved by technology

[0004] At present, traditional gene diagnosis methods include enzyme digestion, restriction fragment length polymorphism (RFLP), direct sequencing, etc. These methods are either not qualitative, or time-consuming and labor-intensive, and require expensive equipment and consumables. Importantly, these methods are difficult to detect multiple mutation sites in different genes at the same time

Although the gene chip method can detect different gene loci at the same time, it requires expensive equipment and consumables, and is not suitable for large-scale clinical promotion, and its application is limited.

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