Hybrid membrane strip, PCR primer and kit for diagnosis of NSHI

A syndrome, membrane strip technology, applied in the determination/inspection of microorganisms, biochemical equipment and methods, DNA/RNA fragments, etc., can solve the problems of time-consuming and laborious, inability to characterize, application limitations, etc., to achieve the convenience of use and price Inexpensive, high-efficiency effects

CN102220434AActive Publication Date: 2011-10-19SHENZHEN YILIFANG BIOTECH CO LTD
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
Publication Date
2011-10-19

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Abstract

The invention discloses a hybrid membrane strip for diagnosis of NSHI (nonsyndromic hearing impairment). The membrane strip comprises a substrate and mutation detection probes, wherein each mutation detection probe respectively comprises a corresponding NSHI gene mutation locus. The gene mutation locus is at least one of the followings: a cDNA35 locus, cDNA176-191 loca, a cDNA235 locus, and cDNA299-300 loca of a GJB2 gene, a cDNA538 locus of a GJB3 gene, a cDNA1555 locus and a cDNA1494 locus of an mtDNA12srRNA gene, and a cDNA2168 locus and an IVS7-2 locus of an SLC26A4 gene; each mutation detection probe comprises sequences of 15-25 bases, and a central section of a sequence comprises a corresponding mutation base of the NSHI gene. The invention has the advantages of high accuracy and low prices, etc.
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Description

technical field

[0001] The present invention relates to a hybrid membrane strip used in medical diagnosis of non-syndromic deafness, and the present invention also relates to a polymerase chain reaction (Polymerase Chain Reaction, abbreviated as The term is PCR) primers; the present invention also relates to a kit for diagnosing non-syndromic deafness. Background technique

[0002] Deafness is one of the most common human sensory system defects. Due to its complex etiology, high incidence and difficult treatment, it has long plagued patients and their surrounding groups, greatly affecting mutual communication and quality of life. The incidence of severe deafness in newborns is as high as 1 / 800~1 / 1000, and nearly 70 million people around the world suffer from hearing loss of more than 55 decibels.

[0003] There are many reasons for deafness, and genetic factors are the main reason. Deafness can be caused by mutations in a single gene or by compound mutations in different g...

Examples

Embodiment Construction

[0024] The present invention will be described in detail below with reference to the accompanying drawings and in combination with preferred specific embodiments.

[0025] 1. Preparation of hybrid membrane strips

[0026] 1.1 Preparation of 9 mutation detection probes and 9 normal control probes

[0027] 18 probes were synthesized according to the sequences in Table 1 and Table 2. The 3' or 5' ends of the mutation detection probes and / or normal control probes were labeled with amino groups. The synthetic method was an existing conventional DNA synthesis method.

[0028] Table 1:

[0029]

[0030] Table 2:

[0031]

[0032] Note: "M" stands for mutation detection probe, "N" stands for normal control probe.

[0033] Each of the mutation detection probes corresponds to a gene mutation site for non-syndromic deafness, and the gene mutation sites for non-syndromic deafness are the following sites: cDNA35, cDNA176-191, cDNA235, cDNA299-300 of the GJB2 gene site, the cDNA53...