Application of ficus formosana maxim extract as drug for preventing skin aging

A technology of skin aging and extracts, applied in skin care preparations, skin diseases, applications, etc., can solve the problems of pathological conditions, pathological conditions deterioration, tissue function and/or structure loss, etc.

Inactive Publication Date: 2013-07-17
TAIWAN SUGAR CORP
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, when MMPs are overactivated, pathological conditions can be caused or aggravated, resulting in loss of tissue function and / or structure

Method used

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  • Application of ficus formosana maxim extract as drug for preventing skin aging
  • Application of ficus formosana maxim extract as drug for preventing skin aging
  • Application of ficus formosana maxim extract as drug for preventing skin aging

Examples

Experimental program
Comparison scheme
Effect test

example 1

[0031] Example 1 Preparation of Taiwan Tianxian fruit extract sample

[0032] This example uses Tianxian fruit produced in Taiwan as the raw material, and the Taiwan Tianxian fruit used has passed the "Taiwan Tianxian fruit pesticide and heavy metal limit standard value".

[0033] Take the Taiwan Tianxian fruit (purchased with dry materials), take the appearance of part of the roots, part of the flakes smaller than 4 cm, and grind to pass through a 30-hole sieve. Test the moisture content according to the operating instructions of the infrared moisture analyzer, and control the moisture content of the Taiwan Tianxian fruit sample to be less than 10%.

[0034] Take 50 grams of Taiwan Tianxian fruit with a moisture content of less than 10% in a 1-liter four-necked flat-bottomed flask, add 500 ml of 100% methanol, heat and reflux for 4 hours, centrifuge and filter, quantify the filtrate volume (V1) and take 1 One milliliter of filtrate was subjected to subsequent HPLC analysis. Add 50...

example 2

[0036] Example 2 Preparation of standard and blank solution

[0037] Weigh 5mg of apigenin and place it in a 2mL volumetric flask, add a little methanol and shake to dissolve it, and then add methanol to the mark to make the standard concentration 2500μg / mL. Then, according to the volume indicated in Table 2, add methanol to serially dilute the standards of different concentrations:

[0038] Table 2 Apigenin standard concentration

[0039] Concentration (μg / mL); ppm 0 100 200 300 400 500

[0040] 2500μg / mL standard (mL) 0 0.08 0.16 0.24 0.32 0.4

[0041] Methanol (mL) 2 1.92 1.84 1.76 1.68 1.6

[0042] The above-mentioned standard solution of different concentrations was analyzed by HPLC, and the concentration of the standard was taken as the abscissa value and the peak area value was the ordinate value for linear regression analysis to obtain an XY line passing through the origin. When the correlation coefficient of the standard curve (R2) is 0.999 ~1, can be used. In this embodiment...

example 3

[0043] Example 3 The HPLC analysis and test of the extract and the standard product of Tianxian fruit of the present invention

[0044] HPLC analysis equipment and methods

[0045] material

[0046] All solvents and reagents are HPLC grade (purchased from Merck). Apigenin (purchased from Sigma) is a standard product of the index component of Tianxian fruit (99% HPLC calibration).

[0047] instrument

[0048] American Waters (WATERS) high performance liquid chromatography system (LC-Module I). The analytical balance can read up to 0.1 mg.

[0049] HPLC analysis conditions

[0050] Chromatographic column is ODS3, C18 column (250×4.6mm, 5μm) (purchased from Varian), column temperature is room temperature, measurement wavelength is 254nm, injection volume is 15μL, mobile phase velocity is 0.8mL / min, and methanol As a sample solvent, the analysis time is 42 minutes. Before sample injection, each sample was filtered with 0.45μm nylon membrane. After the injection, the C18 column is also fi...

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PUM

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Abstract

The invention provides a composition for preventing skin aging. The composition contains ficus formosana maxim extract. The invention also provides a cosmetic which contains ficus formosana maxim extract and can prevent skin aging. The invention also relates to application of ficus formosana maxim extract in preparation of the composition for preventing skin aging.

Description

Technical field [0001] The present invention relates to a celestial fruit extract and its use for preventing skin aging. Background technique [0002] Tianxian fruit belongs to the Ficus genus of Moraceae (Moraceae). It is also called Taiwan Ficus, Xiaoben Niu Rupu, Goat's Milk Tau, Goat's Milk Aberdeen, Mountain Golden Jujube, Shanba Zi, Yudaijiao, etc. Tianxian fruit is distributed in broad-leaved forests or wilderness bushes from the foothills to low-altitude mountainous areas in mainland China, Hong Kong, Vietnam and Taiwan. It can also be found in low-altitude mountainous areas in eastern Taiwan. The hydrangea plant is an evergreen shrub, the whole plant contains white milk, the leaves are alternate with short stalks, and the shape of the leaves is oblong, lanceolate or rhomboid, whole or with sparse serrations, and the leaf shape varies greatly , The leaf color is silver or green, the fruit is ovate or obovate, the fruit is green when immature, with white spots on the surf...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K36/60A61K8/97A23L1/29A61Q19/08A61P17/02A61P17/16A61K125/00A61K131/00A23L33/00
Inventor 杨寄明黄怡仁吕思洁陈嘉琪
Owner TAIWAN SUGAR CORP
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