Eureka AIR delivers breakthrough ideas for toughest innovation challenges, trusted by R&D personnel around the world.

Pichia pastoris wall protein gcw51 and its surface display system and construction method

A construction method and surface display technology, applied in the field of Pichia pastoris wall protein Gcw51 and its constructed surface display system, can solve the problems of low expression of Pir protein and general effect of anchoring protein

Active Publication Date: 2011-12-14
SOUTH CHINA UNIV OF TECH
View PDF4 Cites 1 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the inventors found that the expression level of the Pir protein itself is low, and the effect of the anchor protein as the surface display system of Pichia pastoris is general

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Pichia pastoris wall protein gcw51 and its surface display system and construction method
  • Pichia pastoris wall protein gcw51 and its surface display system and construction method
  • Pichia pastoris wall protein gcw51 and its surface display system and construction method

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

Embodiment 2

Embodiment 3

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention discloses Pichia pastoris wall protein, a surface display system constructed thereby and a construction method thereof. The amino acid sequence of the Pichia pastoris wall protein is SEQ NO:1. The Pichia pastoris wall protein cell surface display system is formed by using the Pichia pastoris wall protein Gcw51 as ankyrin and fixing target protein on the surface of the Pichia pastoris cell. The expression quantity of the wall protein Gcw51 in the Pichia pastoris is very high and is respectively 9 times and 21 times higher than those of the endogenesis ankyrin Pir1 protein and Pir2 protein applied to the Pichia pastoris surface display system.

Description

technical field [0001] The invention relates to the field of biotechnology, in particular to a Pichia pastoris wall protein Gcw51 and its constructed surface display system and construction method. Background technique [0002] At present, the host bacteria that are widely used in the microbial surface display system mainly include phages, bacteria (such as Escherichia coli Escherichia. coli Proteus mirabilis Proteus mirabilis etc.), Saccharomyces cerevisiae ( Saccharomyces cerevisiae ) and Pichia pastoris ( Pichia pastoris ). The anchoring proteins used for surface display generally have the following characteristics: (1) they are firmly anchored on the cell surface and will not easily fall off the cell surface; (2) they can be effectively fused with the target protein sequence without affecting the structure of the target protein and function; (3) have certain resistance to protease. The anchor proteins of the bacterial surface display system mainly include bacter...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Applications(China)
IPC IPC(8): C07K14/39C12N15/31C12N1/19C12N15/81C12R1/84
Inventor 林影张莉周新莹叶燕锐韩双艳郑穗平
Owner SOUTH CHINA UNIV OF TECH
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Eureka Blog
Learn More
PatSnap group products