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High-temperature-resisting lactic acid strain HT1 and application in modulation of silage

A technology of silage and lactic acid bacteria, which is applied to the preservation method, application, and animal feed of animal feed raw materials. It can solve problems such as unsafe operation, strong corrosion, and failure to consider high temperature conditions, and achieve improved fermentation quality and improved fermentation. The effect of quality and great application value

Active Publication Date: 2013-11-13
SOUTH CHINA AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] Adding acidic chemicals such as formic acid during silage can directly reduce pH and improve the fermentation quality of silage, but it is difficult to use in production due to factors such as high cost, strong corrosion, and unsafe operation.
There are also many lactic acid bacteria additives on the market to improve the fermentation quality of silage, but generally do not take into account the high temperature conditions, and the improvement effect is not obvious under high temperature environments

Method used

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  • High-temperature-resisting lactic acid strain HT1 and application in modulation of silage
  • High-temperature-resisting lactic acid strain HT1 and application in modulation of silage
  • High-temperature-resisting lactic acid strain HT1 and application in modulation of silage

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0022] Embodiment 1: screening high temperature resistant good lactic acid bacteria

[0023] Isolate 90 strains of lactic acid bacteria from Kinggrass, Stylophyllum, corn, wild rice and other materials, carry out Gram staining and cell shape observation, and according to the growth temperature (10, 15, 20, 25, 30, 35, 40, 45 , 50°C) and growth pH (3.5, 3.75, 4.0, 4.5, 5.0, 5.5, 6.0, 6.5, 7.0, 7.5, 8.0, 8.5, 9.0) and other experiments screened out lactic acid bacteria HT1 with high temperature resistance, acid resistance and fast growth rate. The results showed that the HT1 strain is a Gram-positive, homofermentative bacillus that can grow at 50°C and pH 3.75, has strong resistance to high temperature and acid, and can ferment most sugars (Table 1).

[0024] Table 1 Characteristics of strain HT1

[0025]

[0026] -: no growth; ±: weak growth; +: growth; ++: good growth.

[0027] Lactic acid bacteria HT1 The physiological and biochemical tests, culture medium ...

Embodiment 2

[0036] Embodiment 2: the identification of lactic acid bacteria

[0037] The strain was cultured overnight at 37°C in 5 mL of MRS medium, the bacterial solution was transferred to a 1.5 mL centrifuge tube, and the bacteria were collected by centrifugation at 10,000 rpm / min for 3 to 5 min, and TE 0.1 (10 mmol / L Tris–HCl, 0.1 mmol / L EDTA, pH 8.0) was washed twice, and DNA was extracted with TIANamp Bacteria DNA Kit (TIANGEN BIOTECH CO., LTD, Beijing, China) and detected at OD600 nm its absorbance value. Then, carry out PCR amplification, the amplification primers of 16S rDNA are 25f (5`-AAC TGA AGA GTT TGA TCC TGG CTC-3`) and 1492r (5`-TAC GGC TAC CTT GTT ACG ACT-3`), RCR The reaction range is 95°C (5 min) - 94°C (30 s) - 55°C (1 min) -72°C (1.5 min) -72°C (10 min), of which 94°C (30 s) - 55°C (1 min) )-72°C (1.5 min) reaction cycle 30 times. The amplified product was sent to Boshang Biotechnology Co., Ltd. (China) for sequencing, and the results were compared on the NCBI g...

Embodiment 3

[0038] Example 3: Effect of adding to silage

[0039] Silage addition experiments were carried out with lightly predried (predried refers to drying to reduce moisture content) and non-predried ryegrass as materials. Cut ryegrass to 1 cm to 2 cm, mix evenly, and add about 1.0×10 strain HT1 per kg of fresh material 8 indivual. The ryegrass feed added with the strain HT1 was put into 30 cm×20 cm polyethylene silage bags, 6 bags for each treatment, 3 bags were stored at 45°C, and 3 bags were stored at 15°C, each bag was about 200 g. (SINBO Vacuum Sealer, Hong Tai Home Electrical Appliance Co. Ltd.) was pumped, sealed, and stored at 45°C for 30 days.

[0040] Table 2 Chemical and microbial components of forage grass before silage

[0041] project undried ryegrass pre-dried ryegrass rice Dry matter (%) 16.37 30.03 50.73 Crude ash (% DM) 9.86 10.28 10.04 Soluble carbohydrates (% DM) 13.7 12.39 11.16 Crude protein (% DM) 14.36 14.87...

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PUM

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Abstract

The invention discloses a high-temperature-resisting lactic acid strain HT1 and a method for modulating silage, belonging to the technical field of microorganism application and silage modulation and process. The strain is HT1, which is identified to be lactobacillusrhamosussp, the collection number of the strain is CGMCC4483, the Genbank accession number of 16SrDNA of the strain is JF414107, and the strain has the main biological characteristics of Gram positive bacilli, homofermentative glucose, acidproof property, quick growth speed, wide breeding temperature range and special high temperature resistance.

Description

technical field [0001] The invention relates to the technical field of microbial application and feed preparation and processing, in particular to the application of lactic acid bacteria in improving the fermentation quality of silage. Background technique [0002] Silage is a long-term storage of fresh pastures, forage crops and other multi-moisture feeds in anaerobic and acidic environments. In recent years, with the rapid development of my country's dairy industry, the production of silage has been increasing rapidly. In 2003, the production of silage in China has exceeded 200 million tons (China Dairy Industry Association, 2003). It can be seen that the production and utilization of silage is of great significance to animal husbandry production, especially for high-yield dairy cows. It can not only ensure the quantity and quality of milk and meat products, but also save feed grain. In addition, through silage, rough or smelly plant materials that are not easy to feed di...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20A23K1/14A23K3/02C12R1/225A23K10/18A23K30/18
Inventor 张建国陈明霞刘秦华
Owner SOUTH CHINA AGRI UNIV
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