Application of urinary laminin in preparation of medical equipment for diagnosing bladder cancer

A technology of laminin and medical equipment, which is applied in the application field of urinary laminin in the preparation of medical equipment for diagnosis of bladder cancer, can solve the problems that urinary laminin has not been reported, and achieve intuitive and reliable judgment of results, rapid detection, and The effect of facilitating self-monitoring of the disease

Inactive Publication Date: 2014-04-30
沈周俊 +2
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, there is no report about the application of urinary laminin in the preparation of medical devices for diagnosing bladder cancer.

Method used

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  • Application of urinary laminin in preparation of medical equipment for diagnosing bladder cancer
  • Application of urinary laminin in preparation of medical equipment for diagnosing bladder cancer
  • Application of urinary laminin in preparation of medical equipment for diagnosing bladder cancer

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0039] There are currently 15 different LN subtypes known, LN1-15. (See: Julia Tzu, MD and M. Peter Marinkovich, MD. BRIDGING STRUCTURE WITH FUNCTION: STRUCTURAL, REGULATORY, AND DEVELOPMENTAL ROLE OF LAMININS. Int J Biochem Cell Biol . 2008 ; 40(2): 199–214.) The present invention detects two clinically commonly used subtypes, LN1 and LN5.

[0040]A total of 182 cases were included, including 92 cases of bladder urothelial carcinoma, 72 cases of other urinary diseases, and 18 healthy controls. The human laminin quantitative enzyme-linked detection kit was used to detect the two subtypes of LN1 and LN5 respectively, and the detection steps were referred to the instruction manual of the human laminin quantitative enzyme-linked detection kit (Shanghai Senxiong Technology Industrial Co., Ltd.).

[0041] 1. ELISA detection results of LN5 subtype:

[0042] 1. Bladder cancer group: 24.24±21.87

[0043] 2. Bladder cancer postoperative group: 13.48±7.79

[0044] 3. Other urinary ...

Embodiment 2

[0067] Example 2 Preparation of gold-labeled antibody complex on test paper

[0068] 1. Colloidal gold preparation

[0069] Add 100ml of 0.005%-0.02% chloroauric acid solution into a round bottom flask and heat to boiling. After boiling, add freshly prepared 0.4%-2% trisodium citrate (Na 3 C 6 h 5 o 7 2H 2 O) 1-2.2ml of aqueous solution, after boiling for 10-25 minutes, continue to stir and cool to room temperature. During this process, it can be seen that the color change of the solution is: golden yellow→black→purple→dark blue→bright red. When the color of the solution completely changes to transparent bright red, the required colloidal gold is obtained. After cooling, put it into a dialysis bag and dialyze it against ultrapure water (1:5000) three times, and finally transfer the dialyzed colloidal gold to a clean glass bottle with a screw cap, and store it in a dark environment at 4°C. (colloidal gold: HAuCl 4 : Sinopharm Chemical Reagent Co., Ltd.; trisodium citra...

Embodiment 3

[0077] Example 3 Preparation of LN1 test paper for rapid diagnosis of bladder cancer

[0078] Please refer to attached figure 1 , with figure 1 It is a schematic diagram of the structure of bladder cancer urinary laminin LN1 diagnostic test paper. The LN1 test paper is provided with a bottom plate 1, and the bottom plate is covered with a sample pad 2, a gold pad 3, a nitrocellulose film layer 4 and an absorbent paper layer 5 in sequence. The base plate 1 is a PVC base plate, and the material of the sample pad 2 is urine-absorbing glass fiber. Preparation of gold gel pad 3: Spray the gold-labeled antibody solution prepared in Example 2 on a glass fiber membrane with a width of 1 cm, with a sample volume of about 2 ul / cm, and dry at 37°C. Preparation of nitrocellulose membrane layer 4: Spray the secondary antibody on the nitrocellulose membrane (NC membrane) as the quality control line (C line). Spray the anti-LN1 polyclonal antibody at a distance of 1cm from the quality ...

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Abstract

The invention relates to the application of urinary laminin (LN1) to preparation of medical equipment for diagnosing bladder cancer. The invention provides an LN1 test paper for diagnosing the bladder cancer, and the application of the LN1 test paper. The invention has the advantages that a new usage of the LN1 is provided, so that a convenient, reliable, non-invasive and inexpensive method is provided for large-scale population screening of the bladder cancer and postoperative self-examination of a bladder cancer patient; the LN1 test paper is rapid in detection, high in sensitivity, strong in specificity, good in stability and simple and convenient in operation, other instruments and equipment are not needed, the results are visually and reliably judged and easy to master, and the patient can conveniently carry out self-monitoring on the condition of the patient; the sensitivity of the test paper is improved, and the specificity of the test paper is also improved; and the LN1 test paper can be used for screening in high risk group and the postoperative follow-up visit for the bladder cancer patient, thus having a wider application scope.

Description

technical field [0001] The present invention relates to the application of urinary laminin, specifically, the application of urinary laminin in the preparation of medical equipment for diagnosing bladder cancer, and a diagnostic test paper for bladder cancer and its application. Background technique [0002] Urothelial carcinoma of the bladder is the most common malignant tumor of the urinary system, the 6th most common tumor in men and the 10th most common tumor in women. The recurrence rate after the first treatment is as high as 80%. Therefore, postoperative follow-up is an important part of treatment. Currently, the diagnosis of bladder cancer mainly relies on urethral cystoscopy and urine exfoliated cytology. However, cystoscopy is traumatic, expensive, and has certain complications. Only about 40% of patients can insist on checking according to the doctor's advice; and cystoscopy is not easy to find early and special bladder cancer lesions. Although urine exfoliated...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N33/574G01N33/558
Inventor 沈周俊张敏光周春洪
Owner 沈周俊
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