Porphyridium culture medium formula and plastic film bag semi-continuous culture method
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A technology of plastic film bag and culture medium formula, which is applied in the field of Porphyridium algae medium formula and plastic film bag semi-continuous culture, which can solve the problems of high cost, shortened growth delay period of algae body, troublesome operation, etc.
What is AI technical title?
AI technical title is built by PatSnap AI team. It summarizes the technical point description of the patent document.
A technology of plastic film bag and culture medium formula, which is applied in the field of Porphyridium algae medium formula and plastic film bag semi-continuous culture, which can solve the problems of high cost, shortened growth delay period of algae body, troublesome operation, etc.
CN102827776BInactive 📅 Publication Date: 2014-07-16LINYI UNIVERSITY
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[0025] Culture medium preparation method
[0026] 1) 920 plant growth stimulant (ie gibberellin) is a white crystalline powder, which is difficult to dissolve in water. It can be fully dissolved with alcohol first (generally, 50 ml of alcohol or 60-degree white wine is used for more than 1 hour per gram), and then Dilute with water according to the required concentration;
[0027] 2) During the process of adding nutrient salts, the water needs to be continuously stirred, and various nutrient salts are added in the order provided in the formula to avoid chemical reactions;
[0028] 3) Various nutrients, especially A 8 Both the trace element solution and the f / 2 vitamin solution need to be made into mother liquor in advance; but the storage time should not exceed 7 days, and it is best to store in a refrigerator at 4°C;
[0029] 4) After adding all the nutrient salts, inflate and stir for 15 minutes before inoculating;
[0030] 5) The purity of all drugs must be chemically pu...
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Abstract
The invention discloses a porphyridium culture medium formula and a plastic film bag semi-continuous culture method, and relates to the field of ocean microalgae culture. The porphyridium culture traditionally adopts an SBM nutrient solution formula, the growth rate is slow, and the yield is low. The experiments of the inventor show that NH4NO3 is taken as a nitrogen source, an appropriate amount of NaHCO3 is added, and gibberellin, A8 trace elements and f / 2 vitamin are supplemented, so that the nutrition is more balanced and complete, the porphyridium growth rate is greatly increased, and the yield can be improved by 78%. The porphyridium culture mode is that the inventor adopts the plastic film bag semi-continuous culture method to replace the conventional open type cement tank batch culture, so microalgae cells are kept with appropriate nutritive salt concentration during the growth process all the time, and the plastic film bag semi-continuous culture method has the characteristics of easiness for operation, difficulty for pollution, easiness for mastering, flexibility, diversity, difficulty for scratching, safety, reliability, good light transmission, low cost, quick growing and high yield.
Description
technical field [0001] The invention belongs to the field of marine microalgae cultivation, and in particular relates to a culture medium formula of Porphyridum and a method for semi-continuous cultivation of plastic film bags. Background technique [0002] Porphyridium cruentum belongs to Rhodophyta, Protoerythrophyta, Porphyrides, Porphyridaceae, and Porphyridium genus. It is a marine single-celled microalgae that presents purple or deep red in different growth stages. It contains Rich in polyunsaturated fatty acid PUFAs such as arachidonic acid AA and eicosapentaenoic acid EPA. AA and EPA are important essential fatty acids in the human body. AA has the functions of regulating cardiac excitability, participating in neuroendocrine, promoting cell division and inhibiting platelet aggregation. The precursor of triene can improve blood lipid quality, reduce cholesterol, blood viscosity and blood sugar concentration, and can effectively treat and prevent coronary heart diseas...
Claims
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