Bionic culture method for cordyceps sobolifera

A cultivation method and technology of cicadae, which is applied in the direction of fungi, etc., can solve the problems of wild cicadae resource scarcity, seasonality, production cannot meet market demand, etc., and achieve the effect of easy harvesting and good air permeability

Active Publication Date: 2014-03-26
ZHEJIANG BIOASIA PHARMA CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Because of its medicinal value, cicadae has been widely recognized by the common people, but due to the scarcity of wild cicadae resources and the seasonality of production, its output is far from meeting the market demand

Method used

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  • Bionic culture method for cordyceps sobolifera
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  • Bionic culture method for cordyceps sobolifera

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0040] The conidia of Paecilomyces cicadae were collected from natural cicada flowers, the spores were diluted by the dilution method, coated on a potato-sucrose-agar plate, and cultured upside down at 25°C for 7 days to obtain a single colony. Pick the strains with vigorous colony growth, dense spores, and no angular change as monospore strains.

[0041] The monospore strain Paecilomyces cicadae was inoculated on the fruiting body formation medium (rice, water, ratio 40:60), and cultured at 22° C. for 30 days. Pick fast-growing, fruiting bodies that produce abundant, stout strains.

[0042] The bacterial strain obtained by screening was transferred to the sporulation medium (bran, silkworm chrysalis powder, water, ratio 40:10:50), cultivated at 25°C for 15 days, collected conidia, and separated them with 0.05% Tween 80 water. Spores made up to 1×10 6 single / ml concentration of spore fluid.

[0043] Soak tussah silkworm pupae in spore liquid for 3 minutes, pick them up, put...

Embodiment 2

[0046] Paecilomyces cicadae conidia were collected from natural cicada flowers, the spores were diluted by the dilution method, coated on a potato-glucose-agar medium plate, and cultured upside down at 25°C for 7 days to obtain monospore colonies. Pick the bacterial strains with vigorous growth, dense spores, and no angular change, and inoculate them on the fruiting body formation medium (millet, water, ratio 35:65), and cultivate them at 22° C. for 30 days. Will grow fast, producing abundant, stout strains with fruiting bodies.

[0047] The bacterial strain obtained by screening was transferred to the spore-producing medium (bran, silkworm chrysalis powder, water, ratio 45:8:47), cultivated at 26°C for 14 days, collected conidia, and separated them with 0.04% Tween 80 water. Spores made up to 3×10 6 / ml concentration of spore liquid.

[0048] Soak silkworm pupae in spore liquid for 2.5 minutes, pick them up, put them in a sterilized container, keep warm at 24°C, humidity 90...

Embodiment 3

[0051] The conidia of Paecilomyces cicadae were collected from natural cicadae flowers, the spores were diluted by the dilution method, coated on a potato-sucrose-agar plate, and cultivated upside down at 25°C for 7 days to obtain monospore strains. Pick the strains with vigorous growth, dense spores, and no angular transformation, and inoculate them on the fruiting body formation medium (barley, water, ratio 40:60), and cultivate them at 22°C for 30 days. Inoculate the fast-growing, fruiting bodies to produce abundant and strong strains on surface-sterilized grasshopper nymphs to maintain the pathogenicity of insects.

[0052] The bacterial strain obtained by screening was transferred to the spore-producing medium (bran, silkworm chrysalis powder, water, ratio 50:5:45), cultivated at 24°C for 13 days, collected conidia, and separated them with 0.03% Tween 80 water. Spores made up to 1×10 7 single / ml concentration of spore fluid.

[0053] Soak the grasshopper nymphs in the s...

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Abstract

The invention discloses a bionic culture method for cordyceps sobolifera, which includes fungus separation, screening, spore culture, infected body impregnation, medium culture, harvest and other steps and culture conditions thereof. The method can simulate the wild ecological environment and overcome the limit of the growth period and season of wild cordyceps sobolifera, mass culture is easy, the adenosine content of the cultured bionic cordyceps sobolifera is higher than the adenosine content of wild cordyceps sobolifera, the quality is high, and the bionic cordyceps sobolifera has extremely high market popularization value.

Description

technical field [0001] The invention belongs to the field of biology, and in particular relates to a method for biomimetic cultivation of cicadas by using the fungus Paecilomyces cicadae (Miquel) Samson, insect bodies and media. Background technique [0002] Cicada is a precious Chinese herbal medicine in my country. Regarding the medicinal history of cicadae, there is a record of processing cicadae as early as Lei Gong's "Lei Gong Pao Shao Lun" in the Northern and Southern Dynasties. "Jingshi Zhenglei Beiji Materia Medica" records that "Cicadas are sweet and cold in taste, non-toxic, and are mainly used for children with convulsions, convulsions, epilepsy, madness, night crying, and heart palpitations." Because the sexual stage belongs to the same species as Cordyceps sinensis, it is often used as a substitute for Cordyceps sinensis. [0003] Cicada is rich in nutrients and biologically active substances. Studies have confirmed that cicada is rich in protein, amino acids,...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/14
Inventor 陈祝安谭悠久曾奕婷吕雯婷盖悦王玉芹孙长胜
Owner ZHEJIANG BIOASIA PHARMA CO LTD
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