Trichoderma viride and application thereof

A technology of Trichoderma viride, strains, applied in applications, fungicides, fungi, etc.

Active Publication Date: 2012-12-26
ENVIRONMENT & PLANT PROTECTION INST CHINESE ACADEMY OF TROPICAL AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The strain has been preserved in the General Microorganism Center of China Committee for the Collection of Microbial Cultures (CGMCC for short) on June 15, 2012.

Method used

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  • Trichoderma viride and application thereof
  • Trichoderma viride and application thereof
  • Trichoderma viride and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0032] Example 1. Isolation and Identification of Trichoderma viride H06CGMCC No.6229

[0033] 1. Isolation of Trichoderma viride H06CGMCC No.6229

[0034] 1) The isolation and screening steps of the strains are as follows: Select the banana plants in the fields with severe Fusarium wilt disease from the banana planting area in Hainan Province, China, and collect root soil samples. Air-dry the soil sample, weigh 10g after sieving, put it into a conical flask filled with 90mL sterile water and glass beads, shake it fully, let it stand for 5min, and dilute it to 1×10 according to the gradient concentration method. -2 , 1×10 -3 , 1×10 -4 . 100ul of each gradient dilution was spread on the Bengal red separation medium. Culture at a constant temperature of 28°C for 4-5 days, pick and purify representative single colonies according to the characteristics of colony morphology, mycelium richness and sporulation, and transfer them to Bengal red slant medium for storage as the strai...

Embodiment 2

[0044] Example 2. Antagonistic effect of Trichoderma viride H06 CGMCC No.6229 on mycelia of Fusarium oxysporum Cuban race 4 (FOC4)

[0045] Test strains: Trichoderma viride (Trichoderma viride) H06CGMCC No.6229 and Trichoderma T-C2.

[0046] The antagonistic effect of Trichoderma viride H06CGMCC No.6229 and Trichoderma T-C2 on FOC4-B2 was tested by plate confrontation method.

[0047] Three treatments were set up in the experiment, namely Trichoderma H06 group, Trichoderma T-C2 group and control group.

[0048] The treatment method of the Trichoderma H06 group is as follows: the Trichoderma viride (Trichoderma viride) H06CGMCC No.6229 and FOC4-B2 were spotted on both edges of the PDA medium at the same time, with a distance of 5cm-6cm, and they were placed upside down in a constant temperature incubator at 28°C for cultivation. Repeat 3 times, regularly observe the inhibitory effect of Trichoderma viride (Trichoderma viride) H06CGMCC No.6229, and record the colony growth radi...

Embodiment 3

[0059] Example 3, Preparation of Trichoderma viride (Trichoderma viride) H06CGMCC No.6229 inoculant

[0060] Trichoderma strains: Trichoderma viride (Trichoderma viride) H06CGMCC No.6229 and Trichoderma T-C2

[0061] 1. First-level plate culture:

[0062] Inoculate the isolated and purified Trichoderma strains on a PDA medium plate, put them in an incubator at 28°C, and cultivate them for 3 to 5 days. When the mycelium grows all over the plate and produces spores, transfer it to a shaker flask for culture;

[0063] Each 1000ml PDA medium is made of the following materials: 200g potato, 10g peptone, 20g glucose, 20g agar, and dilute the medium to 1000mL with water. Its preparation method is as follows: Boil the peeled potatoes cut into small pieces for 20 minutes, then filter with gauze, add other ingredients to the filtrate according to the above formula, dilute to 1000mL with water, and sterilize with damp heat at 121°C for half an hour to obtain the culture medium .

[00...

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PUM

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Abstract

The invention discloses trichoderma viride and application thereof. The trichoderma viride is particularly trichoderma viride H06 with CGMCC No. 6229 which has the high prevention and control effect on banana wilt caused by fusarium oxysporum. sp. cubense specialized form No.4 (FOC), wherein the prevention and control effect on banana wilt can be 88%; and the trichoderma viride H06 with CGMCC No.6229 is simple in cultivation conditions, easy to store and industrially produce and excellent in development application prospect.

Description

technical field [0001] The invention relates to a strain of Trichoderma viride and its application. Background technique [0002] Banana wilt, also known as banana Panama disease and yellow leaf disease, is a devastating disease that destroys vascular bundles and causes plant death. The pathogen is Fusarium oxysporum f.sp.cubense. Fusarium oxysporum Cuban-specific race 4 (FOC4) has caused damage in some countries in Asia and Africa, as well as Australia, and seriously threatens the survival of Cavendish, the world's largest banana cultivar. In the 1960s, Cavendi sh banana replaced Damiha as the world's number one banana variety, but the latter became extinct due to the damage of the banana Fusarium wilt race 1. Therefore, the emergence and spread of FOC4 has aroused great concern in banana growing countries. FOC4 can infect almost all kinds of bananas, such as Cavendish, Damiha, Dwarf banana, and Rhododendron, and is the most harmful. [0003] At present, there is no effe...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/14C12N3/00A01N63/04A01P3/00C12R1/885
Inventor 黄俊生梁昌聪杨腊英吴琳王亚
Owner ENVIRONMENT & PLANT PROTECTION INST CHINESE ACADEMY OF TROPICAL AGRI SCI
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